公开(公告)号：US20170321242A1

公开(公告)日：2017-11-09

申请号：US15657042

申请日：2017-07-21

申请人： DSM IP ASSETS B.V.

发明人： Hubertus Johannes Marie OP DEN CAMP ,  Harry Ramanoedj Harhangi ,  Christiaan Van Der Drift ,  Jacobus Thomas Pronk

IPC分类号： C12P35/00 ,  C12N9/90 ,  C12P17/10 ,  C12P13/00 ,  C12P7/56 ,  C12N9/92 ,  C12N9/12 ,  C12P7/20 ,  C12P7/18 ,  C12P7/06 ,  C12P7/00 ,  C12P5/02 ,  C12P7/46 ,  C12P7/54

CPC分类号： C12P35/00 ,  C12N9/1205 ,  C12N9/90 ,  C12N9/92 ,  C12P5/026 ,  C12P7/00 ,  C12P7/06 ,  C12P7/18 ,  C12P7/20 ,  C12P7/46 ,  C12P7/54 ,  C12P7/56 ,  C12P13/001 ,  C12P17/10 ,  C12Y207/01017 ,  C12Y503/01005 ,  Y02E50/16 ,  Y02E50/17

摘要： The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.

公开(公告)号：US09023629B2

公开(公告)日：2015-05-05

申请号：US13688418

申请日：2012-11-29

申请人： DSM IP Assets B.V.

发明人： Hubertus Johannes Marie Op Den Camp ,  Harry Ramanoedj Harhangi ,  Christiaan Van Der Drift ,  Jacobus Thomas Pronk

IPC分类号： C12P7/06 ,  C12N9/00 ,  C12N9/90 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04 ,  C12P35/00 ,  C12N9/12 ,  C12N9/92 ,  C12P7/00 ,  C12P5/02 ,  C12P7/18 ,  C12P7/20 ,  C12P7/46 ,  C12P7/54 ,  C12P7/56 ,  C12P13/00 ,  C12P17/10

CPC分类号： C12P35/00 ,  C12N9/1205 ,  C12N9/90 ,  C12N9/92 ,  C12P5/026 ,  C12P7/00 ,  C12P7/06 ,  C12P7/18 ,  C12P7/20 ,  C12P7/46 ,  C12P7/54 ,  C12P7/56 ,  C12P13/001 ,  C12P17/10 ,  C12Y207/01017 ,  C12Y503/01005 ,  Y02E50/16 ,  Y02E50/17

摘要： The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.

摘要翻译： 本发明涉及用编码从厌氧真菌获得的真核木糖异构酶的核酸序列转化的宿主细胞。 当表达时，编码木糖异构酶的序列赋予宿主细胞将木糖转化成木酮糖的能力，其可以被宿主细胞进一步代谢。 因此，宿主细胞能够在木糖上生长作为碳源。 宿主细胞优选是真核微生物，例如酵母或丝状真菌。 本发明还涉及用于生产发酵产物如乙醇的方法，其中本发明的宿主细胞使用木糖进行生长和产生发酵产物。 本发明还涉及从厌氧真菌获得的编码真核木糖异构酶和木酮糖激酶的核酸序列。