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    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS
    4.
    发明申请
    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS 有权
    木质纤维素材料的酶解水解和糖的发酵工艺

    公开(公告)号：US20150307903A1

    公开(公告)日：2015-10-29

    申请号：US14440662

    申请日：2013-11-07

    申请人： DSM IP ASSETS B.V.

    发明人： Bertus NOORDAM ,  Michael Petrus Jozef BERKHOUT

    IPC分类号： C12P7/10 ,  C12P19/14 ,  C12P7/56 ,  C12P7/42 ,  C12P7/40 ,  C12P7/54 ,  C12P7/46 ,  C12P7/48 ,  C12P7/18 ,  C12P5/02 ,  C12P7/20 ,  C12P7/16 ,  C12P17/10 ,  C12P35/00 ,  C12P19/02

    CPC分类号： C12P7/10 ,  C12P5/026 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P17/10 ,  C12P19/02 ,  C12P19/14 ,  C12P35/00 ,  C12P2203/00 ,  Y02E50/16 ,  Y02E50/343

    摘要： The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) whereby less than 7.5 mg enzyme composition/g glucan (on dry matter and enzyme as protein) or less than 3.0 mg enzyme composition/g feedstock (on dry matter and enzyme as protein) is used; and e) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and f) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.

    摘要翻译： 本发明涉及一种从木质纤维素材料制备发酵产物的方法，包括以下步骤：a）任选地预处理木素纤维素材料; b）任选地洗涤任选预处理的木质纤维素材料; c）使用包含至少两种纤维素酶的酶组合物对任选洗涤和/或任选预处理的木质纤维素材料进行酶水解，由此所述酶组合物至少包含GH61; d）使用小于7.5mg酶组合物/ g葡聚糖（以干物质和酶为蛋白质）或小于3.0mg酶组合物/ g原料（以干物质和酶为蛋白质）; 和e）水解的木质纤维素材料的发酵以产生发酵产物; 和f）任选地回收发酵产物; 其中在酶水解之前和/或期间，向木质纤维素材料中加入氧气。

    Isolated Fungal Promoters and Gene Transcription Terminators and Methods of Protein and Chemical Production in a Fungus
    9.
    发明申请
    Isolated Fungal Promoters and Gene Transcription Terminators and Methods of Protein and Chemical Production in a Fungus 有权
    分离的真菌启动子和基因转录终止子和真菌中蛋白质和化学生产的方法

    公开(公告)号：US20090068723A1

    公开(公告)日：2009-03-12

    申请号：US12257261

    申请日：2008-10-23

    申请人： Ziyu Dai ,  Linda L. Lasure ,  Jon K. Magnuson

    发明人： Ziyu Dai ,  Linda L. Lasure ,  Jon K. Magnuson

    IPC分类号： C12N1/21 ,  C12N15/11 ,  C12N15/00

    CPC分类号： C12P7/44 ,  C07K14/38 ,  C12N15/80 ,  C12P7/46 ,  C12P7/48 ,  C12P7/56 ,  C12P7/58 ,  C12P17/06 ,  C12P21/005 ,  C12P35/00 ,  C12P37/00

    摘要： The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

    摘要翻译： 本发明包括在天然真菌中差异表达的分离的基因调控元件和基因转录终止子，其表现出相对于表现出第二形态的天然真菌的第一形态。 本发明还包括利用真菌进行蛋白质或化学生产的方法。 通过用重组多核苷酸分子转化真菌产生转化的真菌。 重组多核苷酸分子含有与包含感兴趣的基因的编码区的另一个分子可操作地连接的分离的多核苷酸序列。 基因调控元件和基因转录终止子可以在时间上和空间上调节特定基因的表达，以在转基因真菌中最佳产生感兴趣的化合物。