公开(公告)号：US20190249201A1

公开(公告)日：2019-08-15

申请号：US16309422

申请日：2017-06-13

Applicant: DSM IP ASSETS B.V.

Inventor： Ioannis PAPAPETRIDIS ,  Antonius Jeroen Adriaan VAN MARIS ,  Jacobus Thomas PRONK ,  Paulus Petrus DE WAAL ,  Niels LAURET ,  Maarten Dirk VERHOEVEN ,  Jozef Petrus Johannes SCHMITZ

IPC: C12P7/06 ,  C12R1/645 ,  C12N1/16 ,  C12N9/88 ,  C12N9/12

CPC classification number: C12P7/06 ,  C12N1/16 ,  C12N9/1205 ,  C12N9/88 ,  C12N15/81 ,  C12R1/645 ,  C12Y207/01019 ,  C12Y401/01039 ,  Y02E50/17

Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.

公开(公告)号：US20220389458A1

公开(公告)日：2022-12-08

申请号：US17771333

申请日：2020-11-02

Applicant: DSM IP Assets B.V.

Inventor： Maarten Dirk VERHOEVEN ,  Johannes Andries ROUBOS ,  Noel Nicolaas Maria Elisabeth VAN PEIJ

IPC: C12N15/87 ,  C12N15/80

Abstract: The invention relates to an improved method for the transfection of fungal cells.

公开(公告)号：US20210002651A1

公开(公告)日：2021-01-07

申请号：US16763394

申请日：2018-11-12

Applicant: DSM IP ASSETS B.V.

Inventor： Antonius Jeroen Adriaan VAN MARIS ,  Jacobus Thomas PRONK ,  Maarten Dirk VERHOEVEN

IPC: C12N15/52 ,  C12N9/90 ,  C12N9/12 ,  C12P7/14 ,  C07K14/435

Abstract: The invention relates to a variant of a parent polypeptide which is preferably a hexose transporter, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of SEQ ID NO: 1, comprises a substitution of amino acids N376 and T89, the positions of said amino acids being defined with reference to the amino acid sequence of SEQ ID NO: 1. Said polypeptide is suitable in the production of ethanol from arabinose-rich biomass such as corn stover and corn starch.

公开(公告)号：US20200377846A1

公开(公告)日：2020-12-03

申请号：US16966741

申请日：2019-01-31

Applicant: DSM IP ASSETS B.V.

Inventor： Antonius Jeroen Adriaan VAN MARIS ,  Jacobus Thomas PRONK ,  Maarten Dirk VERHOEVEN ,  Ioannis PAPAPETRIDIS

IPC: C12N1/18 ,  C12N15/52 ,  C12N9/90 ,  C12N9/12 ,  C12N9/92 ,  C12N9/04 ,  C12N1/22 ,  C12P1/02

Abstract: A method for preparing a yeast capable of simultaneously fermenting a pentose and a hexose sugar, comprising: (a) providing a yeast comprising: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway; disruptions of a gene encoding a ribulose-phosphate 3-epimerase and of a gene encoding a glucose-6-phosphate isomerase; one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and a disruption of one or more genes encoding an NADPH dependent 6-phosphogluconate dehydrogenase, (b) subjecting said yeast to evolutionary engineering on a medium comprising a hexose sugar and at least one pentose sugar, selecting for a yeast with improved growth rate obtain an evolved yeast; (d) restoring, in the evolved yeast, one or more of the disrupted genes, or: (d′) identifying genetic permutations in at least part of the genome of the evolved yeast by genome sequencing; (e) constructing an improved pentose and hexose-fermenting yeast comprising one or more said genetic permutations. Also described is a recombinant yeast comprising one or more heterologous genes of a pentose metabolic pathway, and a gene encoding a variant of a parent polypeptide, the variant comprising an amino acid sequence comprising at least one mutation, when aligned with the amino acid sequence in SEQ ID NO: 6.

公开(公告)号：US20200255871A1

公开(公告)日：2020-08-13

申请号：US16858163

申请日：2020-04-24

Applicant: DSM IP ASSETS B.V.

Inventor： Ioannis PAPAPETRIDIS ,  Antonius Jeroen Adriaan VAN MARIS ,  Jacobus Thomas PRONK ,  Paulus Petrus DE WAAL ,  Niels LAURET ,  Maarten Dirk VERHOEVEN ,  Jozef Petrus Johannes SCHMITZ

IPC: C12P7/06 ,  C12N15/81 ,  C12R1/645 ,  C12N1/16 ,  C12N9/88 ,  C12N9/12

Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.