公开(公告)号：US20190194692A1

公开(公告)日：2019-06-27

申请号：US15541665

申请日：2016-01-06

Applicant: DSM IP ASSETS B.V. ,  RIJKSUNIVERSITEIT GRONINGEN

Inventor： Bernard MEIJRINK ,  René VERWAAL ,  Bianca Elisabeth Maria GIELESEN ,  Johannes Andries ROUBOS ,  Carsten POHL ,  Arnold Jacob Mattieu DRIESSEN ,  Roelof Ary Lans BOVENBERG ,  Yvonne Irene NYGÅRD

IPC: C12N15/90 ,  C12N15/11 ,  C12N9/22

CPC classification number: C12N15/902 ,  C12N9/22 ,  C12N15/11 ,  C12N2310/20 ,  C12N2800/80

Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a filamentous fungal host cell.

公开(公告)号：US20240263172A1

公开(公告)日：2024-08-08

申请号：US18594869

申请日：2024-03-04

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Andries ROUBOS ,  Rene VERWAAL ,  Brenda VONK

IPC: C12N15/11 ,  C12N9/22 ,  C12N15/81

CPC classification number: C12N15/11 ,  C12N9/22 ,  C12N15/81 ,  C12N2310/20 ,  C12N2330/51

Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR transient expression construct (CTEC) for a genome editing system.

公开(公告)号：US20200032252A1

公开(公告)日：2020-01-30

申请号：US16500717

申请日：2018-04-04

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Andries ROUBOS ,  René VERWAAL ,  Bianca Elisabeth Maria GIELESEN ,  Brenda VONK

IPC: C12N15/11 ,  C12N15/81 ,  C12N15/90 ,  C12N9/22

Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a self-guiding integration construct for a genome editing system.

公开(公告)号：US20170191089A1

公开(公告)日：2017-07-06

申请号：US15314492

申请日：2015-05-28

Applicant: DSM IP Assets B.V.

Inventor： Zheng ZHAO ,  Bernard MEIJRINK ,  Robertus Antonius Mijindert VAN DER HOEVEN ,  Liang WU ,  Johannes Andries ROUBOS

IPC: C12P7/44 ,  C12N9/10 ,  C12N15/52 ,  C07K14/395 ,  C12P7/62 ,  C12N9/88 ,  C12N9/00

CPC classification number: C12P7/44 ,  C07K14/395 ,  C12N9/0008 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P7/62 ,  C12Y203/03001 ,  C12Y401/01006 ,  C12Y401/01031 ,  C12Y401/01032 ,  C12Y604/01001

Abstract: The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: —a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and —a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate.

公开(公告)号：US20220235378A1

公开(公告)日：2022-07-28

申请号：US17608429

申请日：2020-04-23

Applicant: DSM IP Assets B.V.

Inventor： Brenda VONK ,  Adriana Christina VAN DER HOEK ,  Priscilla ZWARTJENS ,  Johannes Andries ROUBOS

IPC: C12N15/90

Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-assembly gene editing system in a eukaryotic cell.

公开(公告)号：US20200172934A1

公开(公告)日：2020-06-04

申请号：US16780063

申请日：2020-02-03

Applicant: DSM IP ASSETS B.V.

Inventor： René VERWAAL ,  Bernard MEIJRINK ,  Nathalie WIESSENHAAN ,  Brenda VONK ,  Johannes Andries ROUBOS

IPC: C12N15/90 ,  C12N15/113 ,  C12N9/22 ,  C12N15/81 ,  C12N15/10 ,  C12P23/00

Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.

公开(公告)号：US20170314011A1

公开(公告)日：2017-11-02

申请号：US15648983

申请日：2017-07-13

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Andries ROUBOS ,  Herman Jan Pel ,  Bernard Meijrink

IPC: C12N15/10 ,  C12N15/66 ,  C12N15/90

CPC classification number: C12N15/1024 ,  C12N15/66 ,  C12N15/90 ,  C12N2800/30 ,  C12N2800/40 ,  C12N2800/50 ,  C12N2800/80

Abstract: The present invention relates to a method based on the use of restriction enzyme digestion and ligation via the cleavage sites, thereby to prepare two or more standardized expression cassettes.

公开(公告)号：US20230287436A1

公开(公告)日：2023-09-14

申请号：US18187937

申请日：2023-03-22

Applicant: DSM IP ASSETS B.V. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： Eric Mosher YOUNG ,  Amar GHODASARA ,  René VERWAAL ,  Johannes Andries ROUBOS ,  Bianca Elisabeth Maria GIELESEN ,  Brenda VONK ,  Alec A.K. NIELSEN ,  Christopher Ashby VOIGT

IPC: C12N15/66 ,  C12N9/22

CPC classification number: C12N15/66 ,  C12N9/22 ,  C12N2310/20

Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a guide-RNA expression system for a eukaryotic host cell.

公开(公告)号：US20210071174A1

公开(公告)日：2021-03-11

申请号：US17053265

申请日：2019-05-06

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Andries ROUBOS ,  Rene VERWAAL ,  Brenda VONK

IPC: C12N15/11 ,  C12N9/22 ,  C12N15/81

Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR transient expression construct (CTEC) for a genome editing system.

公开(公告)号：US20200165637A1

公开(公告)日：2020-05-28

申请号：US16780389

申请日：2020-02-03

Applicant: DSM IP ASSETS B.V.

Inventor： Bernard MEIJRINK ,  René VERWAAL ,  Bianca Elisabeth Maria GIELESEN ,  Johannes Andries ROUBOS

IPC: C12N15/90 ,  C12N15/63 ,  C12N9/22 ,  C12N15/11

Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.