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    RECOMBINANT YEAST CELL
    3.
    发明申请
    RECOMBINANT YEAST CELL 审中-公开

    公开(公告)号：US20200157489A1

    公开(公告)日：2020-05-21

    申请号：US16621572

    申请日：2018-06-04

    Applicant: DSM IP Assets B.V.

    Inventor： Ioannis PAPAPETRIDIS ,  Jacobus Thomas PRONK

    IPC: C12N1/16 ,  C12N15/52 ,  C12P7/10 ,  C12R1/865

    Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein one or more genes of the non-oxidative branch of the pentose phosphate pathway are overexpressed and/or wherein said yeast cell comprises a deletion or disruption of a glycerol-3-phosphate dehydrogenase (GPD) gene.

    IMPROVED YEAST STRAINS FOR ETHANOL PRODUCTION
    4.
    发明申请
    IMPROVED YEAST STRAINS FOR ETHANOL PRODUCTION 审中-公开

    公开(公告)号：US20200095536A1

    公开(公告)日：2020-03-26

    申请号：US16471367

    申请日：2017-12-18

    Applicant: DSM IP Assets B.V.

    Inventor： Ioannis PAPAPETRIDIS ,  Antonius Jeroen Adriaan VAN MARIS ,  Jacobus Thomas PRONK

    IPC: C12N1/18 ,  C12N9/04 ,  C12N9/02 ,  C12P7/10

    Abstract: This invention relates to a recombinant cell, preferably a recombinant yeast cell comprising: a) a gene coding for an enzyme having glycerol-3-phosphate dehydrogenase activity, wherein said enzyme has a cofactor dependency for at least NADP+ and/or for NADPH; b) a gene encoding an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10); and c) a mutation or disruption in at least one gene selected from the group of GPD1 and GPD2. Said cell is suitable for ethanol production, has a reduced glycerol production at high ethanol yield.

    YEAST CELL CAPABLE OF SIMULTANEOUSLY FERMENTING HEXOSE AND PENTOSE SUGARS
    5.
    发明申请
    YEAST CELL CAPABLE OF SIMULTANEOUSLY FERMENTING HEXOSE AND PENTOSE SUGARS 审中-公开

    公开(公告)号：US20200377846A1

    公开(公告)日：2020-12-03

    申请号：US16966741

    申请日：2019-01-31

    Applicant: DSM IP ASSETS B.V.

    Inventor： Antonius Jeroen Adriaan VAN MARIS ,  Jacobus Thomas PRONK ,  Maarten Dirk VERHOEVEN ,  Ioannis PAPAPETRIDIS

    IPC: C12N1/18 ,  C12N15/52 ,  C12N9/90 ,  C12N9/12 ,  C12N9/92 ,  C12N9/04 ,  C12N1/22 ,  C12P1/02

    Abstract: A method for preparing a yeast capable of simultaneously fermenting a pentose and a hexose sugar, comprising: (a) providing a yeast comprising: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway; disruptions of a gene encoding a ribulose-phosphate 3-epimerase and of a gene encoding a glucose-6-phosphate isomerase; one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and a disruption of one or more genes encoding an NADPH dependent 6-phosphogluconate dehydrogenase, (b) subjecting said yeast to evolutionary engineering on a medium comprising a hexose sugar and at least one pentose sugar, selecting for a yeast with improved growth rate obtain an evolved yeast; (d) restoring, in the evolved yeast, one or more of the disrupted genes, or: (d′) identifying genetic permutations in at least part of the genome of the evolved yeast by genome sequencing; (e) constructing an improved pentose and hexose-fermenting yeast comprising one or more said genetic permutations. Also described is a recombinant yeast comprising one or more heterologous genes of a pentose metabolic pathway, and a gene encoding a variant of a parent polypeptide, the variant comprising an amino acid sequence comprising at least one mutation, when aligned with the amino acid sequence in SEQ ID NO: 6.

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