公开(公告)号：US09597026B2

公开(公告)日：2017-03-21

申请号：US14538557

申请日：2014-11-11

申请人： Deirdre Meldrum ,  Laimonas Kelbauskas ,  Jeff Houkal ,  Roger Johnson ,  Yanqing Tian ,  Cody Youngbull

发明人： Deirdre Meldrum ,  Laimonas Kelbauskas ,  Jeff Houkal ,  Roger Johnson ,  Yanqing Tian ,  Cody Youngbull

IPC分类号： A61B5/1455 ,  A61B5/1459 ,  A61B5/00

CPC分类号： A61B5/1459 ,  A61B5/1455 ,  A61B5/6847

摘要： Novel methods and devices including modified endoscopes that employ fiber optic technology and multiple extrinsic optical sensors for enablement of simultaneous determination of change rates of biological analytes in vivo with single-cell resolution. The devices employ dynamic oscillatory actuation to determine the gradient of analytes of interest along one or multiple directions with respect to the cell. The change rate or flux of the analyte of interest can be easily determined by applying the first Fick's law that relates the flux with the concentration gradient. The dual operation mode of the device markedly increases measurement sensitivity and accuracy.

公开(公告)号：US20150133757A1

公开(公告)日：2015-05-14

申请号：US14538557

申请日：2014-11-11

申请人： Deirdre Meldrum ,  Laimonas Kelbauskas ,  Jeff Houkal ,  Roger Johnson ,  Yanqing Tian ,  Cody Youngbull

发明人： Deirdre Meldrum ,  Laimonas Kelbauskas ,  Jeff Houkal ,  Roger Johnson ,  Yanqing Tian ,  Cody Youngbull

IPC分类号： A61B5/1459 ,  A61B1/07

CPC分类号： A61B5/1459 ,  A61B5/1455 ,  A61B5/6847

摘要： Novel methods and devices including modified endoscopes that employ fiber optic technology and multiple extrinsic optical sensors for enablement of simultaneous determination of change rates of biological analytes in vivo with single-cell resolution. The devices employ dynamic oscillatory actuation to determine the gradient of analytes of interest along one or multiple directions with respect to the cell. The change rate or flux of the analyte of interest can be easily determined by applying the first Fick's law that relates the flux with the concentration gradient. The dual operation mode of the device markedly increases measurement sensitivity and accuracy.

摘要翻译： 包括使用光纤技术的改进的内窥镜和多个外在光学传感器的新型方法和装置，用于实现同时测定生物分析物在体内的单细胞分辨率的变化率。 这些装置采用动态振荡致动来确定相对于电池的一个或多个方向的感兴趣的分析物的梯度。 感兴趣的分析物的变化率或通量可以很容易地通过应用与流量和浓度梯度相关的第一个菲克定律来确定。 器件的双重工作模式显着提高了测量灵敏度和精度。

公开(公告)号：US09181375B2

公开(公告)日：2015-11-10

申请号：US13985260

申请日：2012-02-13

申请人： Yanqing Tian ,  Deirdre Meldrum ,  Xianfeng Zhou ,  Fengyu Su ,  Roger H. Johnson ,  Cody Youngbull

发明人： Yanqing Tian ,  Deirdre Meldrum ,  Xianfeng Zhou ,  Fengyu Su ,  Roger H. Johnson ,  Cody Youngbull

IPC分类号： C08F226/06 ,  C07D498/08 ,  C07D519/00 ,  G01N33/84 ,  C07F15/00 ,  C08F230/04

CPC分类号： C08F226/06 ,  C07D498/08 ,  C07D519/00 ,  C07F15/0093 ,  C08F230/04 ,  C08G2261/226 ,  C08G2261/3223 ,  G01N33/84

摘要： This disclosure relates generally to potassium ion sensors and monomers derived from such sensors. The disclosure also provides for polymers (e.g., random copolymers, nanoparticles, polymer thin films, and sensors) having polymerized monomeric potassium ion sensors as described herein. These compounds and polymers are useful for measuring intracellular and extracellular potassium ion concentrations.

摘要翻译： 本公开一般涉及衍生自这种传感器的钾离子传感器和单体。 本公开还提供了具有如本文所述的聚合的单体钾离子传感器的聚合物（例如，无规共聚物，纳米颗粒，聚合物薄膜和传感器）。 这些化合物和聚合物可用于测量细胞内和细胞外钾离子浓度。

公开(公告)号：US20140200319A1

公开(公告)日：2014-07-17

申请号：US13985260

申请日：2012-02-13

申请人： Yanqing Tian ,  Deirdre Meldrum ,  Xianfeng Zhou ,  Fengyu Su ,  Roger H. Johnson ,  Cody Youngbull

发明人： Yanqing Tian ,  Deirdre Meldrum ,  Xianfeng Zhou ,  Fengyu Su ,  Roger H. Johnson ,  Cody Youngbull

IPC分类号： C08F226/06 ,  C08F230/04 ,  C07D498/08

CPC分类号： C08F226/06 ,  C07D498/08 ,  C07D519/00 ,  C07F15/0093 ,  C08F230/04 ,  C08G2261/226 ,  C08G2261/3223 ,  G01N33/84

摘要： This disclosure relates generally to potassium ion sensors and monomers derived from such sensors. The disclosure also provides for polymers (e.g., random copolymers, nanoparticles, polymer thin films, and sensors) having polymerized monomeric potassium ion sensors as described herein. These compounds and polymers are useful for measuring intracellular and extracellular potassium ion concentrations.

摘要翻译： 本公开一般涉及衍生自这种传感器的钾离子传感器和单体。 本公开还提供了具有如本文所述的聚合的单体钾离子传感器的聚合物（例如，无规共聚物，纳米颗粒，聚合物薄膜和传感器）。 这些化合物和聚合物可用于测量细胞内和细胞外钾离子浓度。

公开(公告)号：US20120301913A1

公开(公告)日：2012-11-29

申请号：US13480243

申请日：2012-05-24

申请人： Cody Youngbull ,  Kevin P. Timms ,  Jakrey Myers ,  Deirdre R. Meldrum

发明人： Cody Youngbull ,  Kevin P. Timms ,  Jakrey Myers ,  Deirdre R. Meldrum

IPC分类号： C12M1/34 ,  G01N21/64

CPC分类号： G01N21/64 ,  G01N21/253 ,  G01N2021/6482

摘要： Microplate assemblies and methods for using same are provided. In accordance with some embodiments, microplate assemblies are provided, the assemblies comprising a microplate well portion that forms a microplate well and that includes at least one sensor in the microplate well that responds to analyte in the well. In accordance with some embodiments, methods for determining a cell consumption rate of an analyte are provided, the methods comprising: positioning a cell in a microplate well having at least one sensor located within the well; providing analyte to the microplate well at a controlled rate; detecting a response of the sensor to the analyte in the microplate well; and determining a cell consumption rate of the analyte based on the response of the sensor to the analyte.

摘要翻译： 提供微孔板组件及其使用方法。 根据一些实施例，提供微孔板组件，所述组件包括形成微板孔的微板孔部分，并且其包括微孔板中的至少一个传感器，其响应井中的分析物。 根据一些实施例，提供了用于确定分析物的细胞消耗速率的方法，所述方法包括：将细胞定位在具有位于所述孔内的至少一个传感器的微孔板中; 以受控的速率向微孔板提供分析物; 检测传感器对微孔板中分析物的响应; 以及基于所述传感器对所述分析物的响应来确定所述分析物的细胞消耗速率。