公开(公告)号：US20120301913A1

公开(公告)日：2012-11-29

申请号：US13480243

申请日：2012-05-24

申请人： Cody Youngbull ,  Kevin P. Timms ,  Jakrey Myers ,  Deirdre R. Meldrum

发明人： Cody Youngbull ,  Kevin P. Timms ,  Jakrey Myers ,  Deirdre R. Meldrum

IPC分类号： C12M1/34 ,  G01N21/64

CPC分类号： G01N21/64 ,  G01N21/253 ,  G01N2021/6482

摘要： Microplate assemblies and methods for using same are provided. In accordance with some embodiments, microplate assemblies are provided, the assemblies comprising a microplate well portion that forms a microplate well and that includes at least one sensor in the microplate well that responds to analyte in the well. In accordance with some embodiments, methods for determining a cell consumption rate of an analyte are provided, the methods comprising: positioning a cell in a microplate well having at least one sensor located within the well; providing analyte to the microplate well at a controlled rate; detecting a response of the sensor to the analyte in the microplate well; and determining a cell consumption rate of the analyte based on the response of the sensor to the analyte.

摘要翻译： 提供微孔板组件及其使用方法。 根据一些实施例，提供微孔板组件，所述组件包括形成微板孔的微板孔部分，并且其包括微孔板中的至少一个传感器，其响应井中的分析物。 根据一些实施例，提供了用于确定分析物的细胞消耗速率的方法，所述方法包括：将细胞定位在具有位于所述孔内的至少一个传感器的微孔板中; 以受控的速率向微孔板提供分析物; 检测传感器对微孔板中分析物的响应; 以及基于所述传感器对所述分析物的响应来确定所述分析物的细胞消耗速率。

公开(公告)号：US20150087007A1

公开(公告)日：2015-03-26

申请号：US14497005

申请日：2014-09-25

申请人： Deirdre Meldrum ,  Roger Johnson ,  Iniyan Soundappa Elango ,  Andrew Shabilla ,  Hong Wang ,  Jakrey Myers ,  Laimonas Kelbauskas ,  Dean Smith ,  Pimwadee Limsirichai

发明人： Deirdre Meldrum ,  Roger Johnson ,  Iniyan Soundappa Elango ,  Andrew Shabilla ,  Hong Wang ,  Jakrey Myers ,  Laimonas Kelbauskas ,  Dean Smith ,  Pimwadee Limsirichai

IPC分类号： G01N33/50 ,  B01L3/00 ,  C23F1/30

CPC分类号： B01L3/502707 ,  B01L3/502761 ,  B01L2300/0645 ,  C12M23/16 ,  C12M41/36 ,  C23F1/02 ,  C23F1/30 ,  C23F1/44

摘要： A microfluidic device useable for performing live cell computed tomography imaging is fabricated with a cover portion including a first wafer with at least one metal patterned thereon, a base portion including a second wafer with at least one metal patterned thereon and negative photoresist defining recesses therein, and a diffusive bonding layer including a negative photoresist arranged to join the cover portion and the base portion. A composition useful in live cell computer topography includes a long-chain polysaccharide at a concentration of from about 0.01% to about 10.0% in cell culture medium for supporting cell life while enabling cell rotation rate to be slowed to a speed commensurate with low light level imaging.

摘要翻译： 用于执行活细胞计算机断层摄影成像的微流体装置制造成具有覆盖部分，所述覆盖部分包括具有图案化的至少一个金属的第一晶片，包括至少一个图案化的金属的第二晶片的基部和其中限定凹部的负光致抗蚀剂， 以及包括负极光致抗蚀剂的漫射粘结层，所述负性光致抗蚀剂被布置成接合盖部分和基部。 用于活细胞计算机地形的组合物包括在细胞培养基中浓度为约0.01％至约10.0％的长链多糖，用于支持细胞寿命，同时使细胞旋转速率降低至与低光级相当的速度 成像。