公开(公告)号：US11014129B2

公开(公告)日：2021-05-25

申请号：US14912440

申请日：2014-06-19

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Sapna Mehtani ,  John Dana Hubbard

IPC: B08B9/027 ,  C07K1/22 ,  A61L2/18 ,  B08B3/08

Abstract: The present invention provides methods for cleaning a Protein A chromatography column employing a media comprising a Protein A ligand derived from the C domain of Staphylococcus aureus, such that the column can be cleaned using both acidic and alkaline solutions.

公开(公告)号：US20160193633A1

公开(公告)日：2016-07-07

申请号：US14912440

申请日：2014-06-19

Applicant: EMD MILLIPORE CORPORATION

Inventor： Nanying Bian ,  Sapna Mehtani ,  John Dana Hubbard

IPC: B08B9/027 ,  B08B3/08 ,  A61L2/18

CPC classification number: B08B9/027 ,  A61L2/18 ,  B08B3/08 ,  C07K1/22

Abstract: The present invention provides methods for cleaning a Protein A chromatography column employing a media comprising a Protein A ligand derived from the C domain of Staphylococcus aureus, such that the column can be cleaned using both acidic and alkaline solutions.

Abstract translation: 本发明提供使用包含衍生自金黄色葡萄球菌C结构域的蛋白A配体的培养基来清洗蛋白A色谱柱的方法，使得可以使用酸性和碱性溶液清洗该柱。