公开(公告)号：US20140329241A1

公开(公告)日：2014-11-06

申请号：US14336816

申请日：2014-07-21

Applicant: General Atomics

Inventor： Chong-Sheng Yuan ,  Xiaoru Chen

IPC: C12Q1/34 ,  C12Q1/68 ,  G01N33/573

CPC classification number: C12Q1/34 ,  C07H15/10 ,  C07H15/18 ,  C07H15/203 ,  C12Q1/42 ,  C12Q1/44 ,  C12Q1/6876 ,  G01N33/573 ,  G01N2333/938 ,  G01N2458/00

Abstract: The present invention provides novel methods for determining the presence or amount of a hydrolytic enzyme in a sample, based on novel substrates for the enzymes, and also provides compositions and methods that provide highly sensitive assay methods for such hydrolytic enzymes.

公开(公告)号：US20140162294A1

公开(公告)日：2014-06-12

申请号：US13707514

申请日：2012-12-06

Applicant: GENERAL ATOMICS

Inventor： Chong-Sheng Yuan ,  Xiaoru Chen ,  Ruffy Baldemeca

IPC: G01N33/82

CPC classification number: G01N33/82

Abstract: The present invention provides methods for assaying a vitamin D moiety in a sample, using a water miscible organic solvent, a specific binding partner that specifically binds to said vitamin D moiety, the binding partner being different from a natural vitamin D binding protein for the vitamin D moiety, and a water soluble polymer that facilitates binding between the specific binding partner and the vitamin D moiety. Kits and reaction mixtures for assaying a vitamin D moiety in a sample are also provided.

Abstract translation: 本发明提供了使用水混溶性有机溶剂，与所述维生素D部分特异性结合的特异性结合配偶体来测定样品中的维生素D部分的方法，所述结合配偶体不同于维生素的天然维生素D结合蛋白 D部分和促进特异性结合配偶体与维生素D部分之间的结合的水溶性聚合物。 还提供了用于测定样品中维生素D部分的试剂盒和反应混合物。