Fluorescent resonance energy transfer probes
    4.
    发明申请
    Fluorescent resonance energy transfer probes 审中-公开
    荧光共振能量转移探针

    公开(公告)号:US20050042618A1

    公开(公告)日:2005-02-24

    申请号:US10635171

    申请日:2003-08-06

    CPC分类号: C12Q1/6818

    摘要: Compositions, reaction mixtures, and kits, as well as methods for their use, comprising a pair of FRET hybridization probes hybridizing adjacently to a target nucleic acid sequence, each hybridization probe comprising (i) a nucleotide sequence entity which is substantially complementary to the sequence of the target nucleic acid, (ii) a fluorescent entity, being either a FRET donor entity or a FRET acceptor entity, and (iii) a spacer entity connecting the nucleotide sequence entity and the fluorescent entity. The intensity of fluorescence emission from the FRET donor entity and from the FRET acceptor entity is not substantially affected by quenching activity of nucleotide residues present in the sequence of the target nucleic acid or in the nucleotide sequence entities of the hybridization probes.

    摘要翻译: 组合物,反应混合物和试剂盒及其使用方法包括与靶核酸序列相邻杂交的一对FRET杂交探针,每个杂交探针包含(i)与该序列基本互补的核苷酸序列实体 的靶核酸,(ii)作为FRET供体实体或FRET受体实体的荧光实体,和(iii)连接核苷酸序列实体和荧光实体的间隔实体。 来自FRET供体实体和FRET受体实体的荧光发射的强度基本上不受存在于靶核酸序列或杂交探针的核苷酸序列实体中的核苷酸残基的猝灭活性的影响。

    Fret process
    5.
    发明申请
    Fret process 审中-公开
    反应过程

    公开(公告)号:US20070184453A1

    公开(公告)日:2007-08-09

    申请号:US10678440

    申请日:2003-10-01

    IPC分类号: C12Q1/68 C12P19/34 C07H21/04

    CPC分类号: C12Q1/6818

    摘要: The present invention is directed to hybridization probes hybridizing adjacently to another at a target nucleic acid sequence, wherein one member of said hybridization probes comprises (i) a nucleotide sequence entity which is substantially complementary to the sequence of the target nucleic acid, (ii) a fluorescent entity being either a FRET donor entity or a FRET acceptor entity, and (iii) a spacer entity connecting the nucleotide sequence entity and the fluorescent entity.

    摘要翻译: 本发明涉及在目标核酸序列上与另一种相邻地杂交的杂交探针,其中所述杂交探针的一个成员包含(i)与靶核酸序列基本上互补的核苷酸序列实体,(ii) 荧光实体是FRET供体实体或FRET受体实体,和(iii)连接核苷酸序列实体和荧光实体的间隔实体。

    RELATIVE QUANTIFICATION ANALYSIS OF MULTI-PARAMETRIC PCR EXPERIMENTS
    6.
    发明申请
    RELATIVE QUANTIFICATION ANALYSIS OF MULTI-PARAMETRIC PCR EXPERIMENTS 审中-公开
    多参数PCR实验的相对量化分析

    公开(公告)号:US20120070840A1

    公开(公告)日:2012-03-22

    申请号:US13224423

    申请日:2011-09-02

    IPC分类号: C12Q1/68

    摘要: An analysis method for quantitative PCR experiments including multiple genes of interest, multiple samples and multiple conditions is provided. For calculating the relative expression status of multiple target genes from multiple samples under multiple different conditions, a calculation method based on up to three different parameters is performed. In addition to normalization against one or multiple reference gene(s) and normalization against a reference sample (calibrator), a third normalization step against a base value is performed in a target-, sample- and condition-specific manner.

    摘要翻译: 提供了包括感兴趣的多个基因,多个样品和多个条件的定量PCR实验的分析方法。 为了计算多个不同条件下多个样本的多个目标基因的相对表达状态,采用基于三个不同参数的计算方法。 除了针对一个或多个参考基因的标准化和针对参考样品(校准物)的归一化之外,针对基础值的第三归一化步骤以目标,样品和条件特异性方式进行。