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公开(公告)号:US20110151550A1
公开(公告)日:2011-06-23
申请号:US13023407
申请日:2011-02-08
申请人: Gregor Sagner , Ingrid Bechler , Jochen Bolte , Dieter Heindl , Hans-Peter Josel , Martin Gutekunst , Rudolf Sebl , Christoph Mueller
发明人: Gregor Sagner , Ingrid Bechler , Jochen Bolte , Dieter Heindl , Hans-Peter Josel , Martin Gutekunst , Rudolf Sebl , Christoph Mueller
IPC分类号: C12M1/34
CPC分类号: C12Q1/6851 , G01N2021/6421 , C12Q2565/101 , C12Q2561/113 , C12Q2537/143
摘要: The invention is directed to a system for performing multi-color real time PCR, comprising a flexible real time PCR instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixture which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.
摘要翻译: 本发明涉及用于进行多色实时PCR的系统,其包括灵活的实时PCR仪器和用于进行多重PCR的特定组合物或反应混合物:特别地,本发明涉及一种组合物或反应混合物,其中 包含至少3个,优选4-5个,最优选正确的4对FRET杂交探针。 每对所述杂交探针由携带FRET供体部分的FRET供体探针和携带具有550和710nm之间的发射最大值的FRET受体部分的FRET受体探针组成。
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公开(公告)号:US08137616B2
公开(公告)日:2012-03-20
申请号:US10549648
申请日:2004-04-01
申请人: Gregor Sagner , Ingrid Bechler , Jochen Bolte , Dieter Heindl , Hans-Peter Josel , Martin Gutekunst , Rudolf Sebl , Christoph Mueller
发明人: Gregor Sagner , Ingrid Bechler , Jochen Bolte , Dieter Heindl , Hans-Peter Josel , Martin Gutekunst , Rudolf Sebl , Christoph Mueller
CPC分类号: C12Q1/6851 , G01N2021/6421 , C12Q2565/101 , C12Q2561/113 , C12Q2537/143
摘要: The invention is directed to a system for performing multi-color real time PCT, comprising a flexible real time PCT instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixture which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.
摘要翻译: 本发明涉及用于执行多色实时PCT的系统,其包括柔性实时PCT仪器和用于进行多重PCR的特定组合物或反应混合物:特别地,本发明涉及一种组合物或反应混合物,其中 包含至少3个,优选4-5个,最优选正确的4对FRET杂交探针。 每对所述杂交探针由携带FRET供体部分的FRET供体探针和携带具有550和710nm之间的发射最大值的FRET受体部分的FRET受体探针组成。
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公开(公告)号:US20060269922A1
公开(公告)日:2006-11-30
申请号:US10549648
申请日:2004-04-01
申请人: Gregor Sagner , Ingrid Bechler , Jochen Bolte , Dieter Heindl , Hans-Peter Josel , Martin Gutekunst , Rudolf Sebl , Christoph Mueller
发明人: Gregor Sagner , Ingrid Bechler , Jochen Bolte , Dieter Heindl , Hans-Peter Josel , Martin Gutekunst , Rudolf Sebl , Christoph Mueller
CPC分类号: C12Q1/6851 , G01N2021/6421 , C12Q2565/101 , C12Q2561/113 , C12Q2537/143
摘要: The invention is directed to a system for performing multi-color real time PCT, comprising a flexible real time PCT instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixtuer which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.
摘要翻译: 本发明涉及用于执行多色实时PCT的系统,其包括灵活的实时PCT仪器和用于进行多重PCR的特定组合物或反应混合物:特别地,本发明涉及一种组合物或反应混合器,其组合物或反应混合器 包含至少3个,优选4-5个,最优选正确的4对FRET杂交探针。 每对所述杂交探针由携带FRET供体部分的FRET供体探针和携带具有550和710nm之间的发射最大值的FRET受体部分的FRET受体探针组成。
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公开(公告)号:US20050042618A1
公开(公告)日:2005-02-24
申请号:US10635171
申请日:2003-08-06
申请人: Dieter Heindl , Hans-Peter Josel , Gregor Sagner , Ingrid Bechler
发明人: Dieter Heindl , Hans-Peter Josel , Gregor Sagner , Ingrid Bechler
IPC分类号: G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/542 , G01N33/566 , G01N33/58 , C07H21/04
CPC分类号: C12Q1/6818
摘要: Compositions, reaction mixtures, and kits, as well as methods for their use, comprising a pair of FRET hybridization probes hybridizing adjacently to a target nucleic acid sequence, each hybridization probe comprising (i) a nucleotide sequence entity which is substantially complementary to the sequence of the target nucleic acid, (ii) a fluorescent entity, being either a FRET donor entity or a FRET acceptor entity, and (iii) a spacer entity connecting the nucleotide sequence entity and the fluorescent entity. The intensity of fluorescence emission from the FRET donor entity and from the FRET acceptor entity is not substantially affected by quenching activity of nucleotide residues present in the sequence of the target nucleic acid or in the nucleotide sequence entities of the hybridization probes.
摘要翻译: 组合物,反应混合物和试剂盒及其使用方法包括与靶核酸序列相邻杂交的一对FRET杂交探针,每个杂交探针包含(i)与该序列基本互补的核苷酸序列实体 的靶核酸,(ii)作为FRET供体实体或FRET受体实体的荧光实体,和(iii)连接核苷酸序列实体和荧光实体的间隔实体。 来自FRET供体实体和FRET受体实体的荧光发射的强度基本上不受存在于靶核酸序列或杂交探针的核苷酸序列实体中的核苷酸残基的猝灭活性的影响。
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公开(公告)号:US20070184453A1
公开(公告)日:2007-08-09
申请号:US10678440
申请日:2003-10-01
申请人: Gregor Sagner , Dieter Heindl , Ingrid Bechler , Christina Krause
发明人: Gregor Sagner , Dieter Heindl , Ingrid Bechler , Christina Krause
CPC分类号: C12Q1/6818
摘要: The present invention is directed to hybridization probes hybridizing adjacently to another at a target nucleic acid sequence, wherein one member of said hybridization probes comprises (i) a nucleotide sequence entity which is substantially complementary to the sequence of the target nucleic acid, (ii) a fluorescent entity being either a FRET donor entity or a FRET acceptor entity, and (iii) a spacer entity connecting the nucleotide sequence entity and the fluorescent entity.
摘要翻译: 本发明涉及在目标核酸序列上与另一种相邻地杂交的杂交探针,其中所述杂交探针的一个成员包含(i)与靶核酸序列基本上互补的核苷酸序列实体,(ii) 荧光实体是FRET供体实体或FRET受体实体,和(iii)连接核苷酸序列实体和荧光实体的间隔实体。
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6.
公开(公告)号:US20120070840A1
公开(公告)日:2012-03-22
申请号:US13224423
申请日:2011-09-02
申请人: Ingrid Bechler , Gregor Sagner
发明人: Ingrid Bechler , Gregor Sagner
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6851 , G16B40/00 , C12Q2545/101 , C12Q2545/113
摘要: An analysis method for quantitative PCR experiments including multiple genes of interest, multiple samples and multiple conditions is provided. For calculating the relative expression status of multiple target genes from multiple samples under multiple different conditions, a calculation method based on up to three different parameters is performed. In addition to normalization against one or multiple reference gene(s) and normalization against a reference sample (calibrator), a third normalization step against a base value is performed in a target-, sample- and condition-specific manner.
摘要翻译: 提供了包括感兴趣的多个基因,多个样品和多个条件的定量PCR实验的分析方法。 为了计算多个不同条件下多个样本的多个目标基因的相对表达状态,采用基于三个不同参数的计算方法。 除了针对一个或多个参考基因的标准化和针对参考样品(校准物)的归一化之外,针对基础值的第三归一化步骤以目标,样品和条件特异性方式进行。
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