公开(公告)号：US20230001412A1

公开(公告)日：2023-01-05

申请号：US17782598

申请日：2020-01-21

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Alexander GOVYADINOV ,  Hilary Ely ,  Brett E DAHLGREN ,  Si-lam J. CHOY ,  Erik TORNIAINEN

IPC: B01L3/00 ,  C12Q1/6844 ,  B01L7/00 ,  G01N21/64

Abstract: A microfluidic reaction chamber with a reaction chamber circuit includes a microfluidic reaction chamber to contain a reaction fluid for amplification of nucleic acids, and a reaction chamber circuit disposed within the microfluidic reaction chamber. The microfluidic reaction chamber includes a base wall, a top wall parallel to the base wall and defined in part by a transparent lid, a first side wall, and a second side wall. The reaction chamber circuit is disposed within the microfluidic reaction chamber, and includes a top surface, a bottom surface, a first side wall, and a second side wall. The reaction chamber circuit is in fluidic contact with the reaction fluid and includes a photodetector to detect a fluorescence signal from a labeled fluorescent tag in the reaction fluid.

公开(公告)号：US20230285964A1

公开(公告)日：2023-09-14

申请号：US17691214

申请日：2022-03-10

Applicant: Hewlett-Packard Development Company L.P.

Inventor： David OLSEN ,  Si-lam J. CHOY ,  Paul Mark HAINES ,  Justin M. ROMAN

IPC: B01L3/00

CPC classification number: B01L3/502746 ,  B01L2300/048 ,  B01L2400/0605 ,  B01L2400/0622 ,  B01L2400/082 ,  B01L2400/0487

Abstract: A metered volume microfluidic device can include fluid flow microfluidics. The fluid flow microfluidics can include an inflow channel, a metered volume chamber positioned to receive working fluid from the inflow channel, a metered volume outflow channel positioned to receive and direct a metered volume of the working fluid when discharged from the metered volume chamber, and a capillary check valve. The capillary check valve can allow excess working fluid to exit the metered volume chamber when filling the metered volume chamber via the inflow channel. The capillary check valve can also prevent excess working fluid that has passed there through from being reintroduced into the metered volume chamber when the working fluid is discharged into the metered volume outflow channel.

公开(公告)号：US20220314215A1

公开(公告)日：2022-10-06

申请号：US17642265

申请日：2020-05-08

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Hilary ELY ,  Si-lam J. CHOY

IPC: B01L3/00 ,  G01N35/00 ,  C12N15/10

Abstract: A vertically layered fluid column can include a plurality of fluids positioned in fluid layers, a density-differential interface along which two fluids from the plurality of fluids are positioned, and a capillary force-supported interface along which two fluids from the plurality of the fluids are positioned.