公开(公告)号：US20220275377A1

公开(公告)日：2022-09-01

申请号：US17671571

申请日：2022-02-14

Applicant: Inscripta, Inc.

Inventor： Brian Chaikind ,  Aamir Mir

IPC: C12N15/62 ,  C12N5/071 ,  C12N9/22 ,  C12N15/11 ,  C12N15/86 ,  C12N15/90

Abstract: The present disclosure provides compositions of matter, methods and instruments for nucleic acid-guided nickase/reverse transcriptase fusion enzyme editing of nucleic acids in live mammalian cells.

公开(公告)号：US20250084392A1

公开(公告)日：2025-03-13

申请号：US18725912

申请日：2023-01-04

Applicant: INSCRIPTA, INC.

Inventor： Brian Chaikind ,  Christopher Lim

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: This invention relates to compositions of matter, methods and instruments for directly recruiting repair templates to CRISPR nucleases to stimulate homology-directed repair. Molecular “tethers” are described which result in an increase in the local concentration of repair templates at the site of the double-strand break made by a nuclease, thereby enhancing the rate of homology directed repair and suppressing undesired edits.

公开(公告)号：US20220298502A1

公开(公告)日：2022-09-22

申请号：US17696131

申请日：2022-03-16

Applicant: Inscripta, Inc.

Inventor： Brian Chaikind

IPC: C12N15/10 ,  C12N9/22

Abstract: The present disclosure provides compositions and methods to increase the percentage of edited cells in a cell population when employing nucleic-acid guided editing, as well as automated instruments for performing these methods.

公开(公告)号：US20250034595A1

公开(公告)日：2025-01-30

申请号：US18714685

申请日：2022-12-01

Applicant: Inscripta, Inc.

Inventor： Brian Chaikind ,  Alex Hutagalung ,  Janine Mok

IPC: C12N15/90 ,  C12N9/12 ,  C12N9/22 ,  C12N15/11

Abstract: The present disclosure provides compositions of matter, methods and instruments for nucleic acid-guided nickase/reverse transcriptase fusion enzyme editing of nucleic acids in live mammalian cells, and for tracking of editing events.

公开(公告)号：US11884924B2

公开(公告)日：2024-01-30

申请号：US17671571

申请日：2022-02-14

Applicant: Inscripta, Inc.

Inventor： Brian Chaikind ,  Aamir Mir

IPC: C12N15/62 ,  C12N5/071 ,  C12N9/22 ,  C12N15/11 ,  C12N15/90 ,  C12N15/86

CPC classification number: C12N15/62 ,  C12N5/0687 ,  C12N9/22 ,  C12N15/11 ,  C12N15/86 ,  C12N15/907 ,  C12Y301/21004 ,  C12N2310/20 ,  C12N2740/15043 ,  C12N2800/40 ,  C12N2800/80

Abstract: The present disclosure provides compositions of matter, methods and instruments for nucleic acid-guided nickase/reverse transcriptase fusion enzyme editing of nucleic acids in live mammalian cells.

公开(公告)号：US20240043852A1

公开(公告)日：2024-02-08

申请号：US18491049

申请日：2023-10-20

Applicant: Inscripta, Inc.

Inventor： Brian Chaikind ,  Aamir Mir

IPC: C12N15/62 ,  C12N5/071 ,  C12N9/22 ,  C12N15/11 ,  C12N15/90 ,  C12N15/86

CPC classification number: C12N15/62 ,  C12N5/0687 ,  C12Y301/21004 ,  C12N9/22 ,  C12N15/11 ,  C12N15/907 ,  C12N15/86 ,  C12N2740/15043 ,  C12N2310/20 ,  C12N2800/80 ,  C12N2800/40

Abstract: The present disclosure provides compositions of matter, methods and instruments for nucleic acid-guided nickase/reverse transcriptase fusion enzyme editing of nucleic acids in live mammalian cells.