公开(公告)号：US11965154B2

公开(公告)日：2024-04-23

申请号：US16545097

申请日：2019-08-20

Applicant: Inscripta, Inc.

Inventor： Eileen Spindler ,  Isaac Wagner ,  Clint Davis ,  Julia Swavola ,  Phillip Belgrader

IPC: C12N1/20 ,  B01L3/00 ,  C12M1/00 ,  C12M1/42 ,  C12M3/00 ,  C12M3/04 ,  C12N9/22 ,  C12N13/00 ,  G01N35/00 ,  G01N35/10

CPC classification number: C12N1/20 ,  B01L3/502 ,  C12M27/10 ,  C12M29/04 ,  C12M35/00 ,  C12M35/02 ,  C12M45/00 ,  C12N9/22 ,  C12N13/00 ,  G01N35/00722 ,  G01N35/10 ,  B01L2200/16 ,  B01L2300/087 ,  C12M23/44 ,  G01N2035/009

Abstract: The present disclosure provides modules, instruments and methods to enrich for cells edited via nucleic acid-guided nuclease editing of live cells.

公开(公告)号：US11697826B2

公开(公告)日：2023-07-11

申请号：US16421783

申请日：2019-05-24

Applicant: Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/11 ,  C12N15/10 ,  C12N15/63 ,  C12N15/113 ,  C07K14/195

CPC classification number: C12N15/902 ,  C07K14/195 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/905 ,  C12N15/907 ,  C07K2319/85 ,  C12N2310/20 ,  C12N2800/22 ,  C12N2800/80

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20230183683A1

公开(公告)日：2023-06-15

申请号：US18166868

申请日：2023-02-09

Applicant: Inscripta, Inc.

Inventor： Tian TIAN ,  Eileen SPINDLER ,  Clint DAVIS ,  Charles JOHNSON ,  Andrew GARST

IPC: C12N15/10 ,  C12N15/11 ,  C12N9/22 ,  C12N15/63 ,  C12N15/70

CPC classification number: C12N15/1082 ,  C12N15/111 ,  C12N9/22 ,  C12N15/63 ,  C12N15/70 ,  C12N2310/20 ,  C12N2820/002

Abstract: The present disclosure provides compositions of matter, methods, modules and automated multi-module instrumentation for performing editing of live cells followed by curing of editing and engine vectors from prior rounds of editing, followed by curing of the curing vector.

公开(公告)号：US20230174961A1

公开(公告)日：2023-06-08

申请号：US18101543

申请日：2023-01-25

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Christian Siltanen

IPC: C12N9/22 ,  C12N15/90 ,  C12N15/11

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N15/902 ,  C12N2310/20

Abstract: The present disclosure relates to automated multi-module instruments, compositions and methods for performing nucleic acid-guided nuclease editing; specifically, the disclosure provides nucleic acid cassettes, plasmids, vectors, and compositions comprising the same that employ homologous recombination for genome engineering by having a CRISPR nuclease cause a specific DSB while tethered to a repair nucleic acid.

公开(公告)号：US20230136423A1

公开(公告)日：2023-05-04

申请号：US17975844

申请日：2022-10-28

Applicant: Inscripta, Inc.

Inventor： Patrick WESTFALL ,  Catherine BARANOWSKI

IPC: C12N15/10 ,  C12N9/22 ,  C12N15/11 ,  C12N15/72 ,  C12N1/20

Abstract: The present disclosure relates to methods and compositions for nuclease-mediated plasmid integration into the genome of a population of live cells, as well as automated multi-module instruments for performing these methods and using these compositions.

公开(公告)号：US11634719B2

公开(公告)日：2023-04-25

申请号：US17676210

申请日：2022-02-20

Applicant: Inscripta, Inc.

Inventor： Charles Johnson ,  Tian Tian ,  Eileen Spindler

IPC: C12N15/70 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.

公开(公告)号：US11597923B2

公开(公告)日：2023-03-07

申请号：US17690737

申请日：2022-03-09

Applicant: Inscripta, Inc.

Inventor： Daniel Held

IPC: C12N15/10 ,  C12N15/63 ,  C12N15/11

Abstract: The present disclosure relates to compositions, methods, modules and automated integrated instrumentation for multiplex delivery of “landing pad” edits into the genomes of a population of live cells. The landing pads then may be leveraged to insert very large DNA sequences into the genomes of the population of live cells.

公开(公告)号：US11597921B2

公开(公告)日：2023-03-07

申请号：US17693233

申请日：2022-03-11

Applicant: Inscripta, Inc.

Inventor： Jorge Bernate ,  Kevin Ness ,  Phillip Belgrader ,  Don Masquelier ,  Ryan Gill

IPC: C12M3/00 ,  C12M1/00 ,  C12N15/10 ,  C40B40/02 ,  C12M1/26 ,  C12M1/42 ,  C12M1/34 ,  C12Q1/6865 ,  C12M1/12 ,  C12M1/36 ,  C12N9/22 ,  C12N15/11

Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

公开(公告)号：US11591592B2

公开(公告)日：2023-02-28

申请号：US17239540

申请日：2021-04-23

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Nathan Bade ,  Christian Siltanen ,  Aamir Mir ,  Xi-Jun Chen ,  Janine Mok ,  Burak Dura ,  Bruce Chabansky ,  David Stumbo ,  Eric Smith ,  Jorge Bernate

IPC: C12N15/11 ,  C12N15/10 ,  C12M1/34 ,  C12M1/26 ,  C12N15/113 ,  C12N15/86 ,  C12M3/00 ,  C12M3/04 ,  C12M1/00 ,  C12N5/074 ,  C12N15/88 ,  C12N15/90 ,  B01L3/00 ,  B01L7/00 ,  C12M1/32 ,  C12M3/06 ,  C12N9/22

Abstract: This invention relates to compositions of matter, methods, modules and automated, end-to-end closed instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells. The disclosed compositions and method entail making “reagent bundles” comprising many (hundreds of thousands to millions) clonal copies of an editing cassette and delivering or co-localizing the reagent bundles with live mammalian cells such that the editing cassettes edit the cells and the edited cells continue to grow.

公开(公告)号：US11584928B2

公开(公告)日：2023-02-21

申请号：US16295393

申请日：2019-03-07

Applicant: The Regents of the University of Colorado ,  Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb ,  Marcelo Colika Bassalo ,  Ramsey Ibrahim Zeitoun

IPC: C12N15/10 ,  C12N15/11

Abstract: Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.