公开(公告)号：US20250109428A1

公开(公告)日：2025-04-03

申请号：US18899460

申请日：2024-09-27

Applicant: Illumina, Inc.

Inventor： Olivia Ghazinejad ,  Fei Shen ,  Kyle Bentz ,  Rohit Subramanian ,  Eric Brustad ,  Lena Storms ,  Xiaoyu Ma ,  Mathieu Lessard-Viger ,  Jeffrey Brodin

IPC: C12Q1/6806 ,  B01L3/00 ,  C12Q1/6844

Abstract: In some examples, a hybrid particle for use in capturing a polynucleotide may include a scaffold molecule including a seeding primer and a plurality of first moieties, and a nanoparticle including a plurality of second moieties. The scaffold molecule is coupled to the nanoparticle via interactions between the plurality of first moieties and the plurality of second moieties.

公开(公告)号：US20240352510A1

公开(公告)日：2024-10-24

申请号：US18633966

申请日：2024-04-12

Applicant: Illumina, Inc.

Inventor： Mathieu Lessard-Viger ,  Rebecca Turk-MacLeod ,  Vanessa Montaño-Machado ,  Jeffrey Fisher ,  Rohit Subramanian ,  Krishnarjun Sarkar ,  Sahngki Hong ,  Weixian Xi ,  Brandon Wenning ,  Lewis Kraft ,  Wayne George ,  Brian Mather ,  Allison Meade ,  John Daly

IPC: C12Q1/6848 ,  C12Q1/6855

CPC classification number: C12Q1/6848 ,  C12Q1/6855

Abstract: In some examples, a structure is contacted with polynucleotides having a variety of lengths and each including first and second adapters. The structure includes a substrate including first and second regions spaced apart from one another by a gap of at least 100 nm, a first set of capture primers coupled to the first region of the substrate, and a second set of capture primers coupled to the second region of the substrate. The first adapter of the polynucleotide is hybridized to a capture primer of the first set of capture primers. Based on that polynucleotide being sufficiently long to bridge the gap, it is amplified using the first and second sets of capture primers. Based upon that polynucleotide being insufficiently long to bridge the gap, it is not amplified. Optionally, a wall may be disposed in the gap.