公开(公告)号：US20240263229A1

公开(公告)日：2024-08-08

申请号：US18566360

申请日：2022-12-15

Applicant: Illumina, Inc.

Inventor： Xiaoyu Ma ,  Mathieu Lessard-Viger ,  Jeffrey Fisher ,  Jonathan Boutell

IPC: C12Q1/6874 ,  C12N15/10 ,  C12Q1/42 ,  C12Q1/6834 ,  C12Q1/6844

CPC classification number: C12Q1/6874 ,  C12N15/1068 ,  C12Q1/42 ,  C12Q1/6834 ,  C12Q1/6844 ,  G01N2333/916

Abstract: The present disclosure is generally directed to strategies for template capture and amplification during sequencing.

公开(公告)号：US20230416435A1

公开(公告)日：2023-12-28

申请号：US18330298

申请日：2023-06-06

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Gianluca Andrea Artioli ,  Timothy J.N. Beech ,  Mathieu Lessard-Viger ,  Rebecca Turk-MacLeod ,  Brian D. Mather ,  Weixian Xi ,  Xavier von Hatten

IPC: C08F220/56 ,  C08J3/075 ,  C12Q1/6869

CPC classification number: C08F220/56 ,  C08J3/075 ,  C12Q1/6869 ,  C08J2333/26

Abstract: A co-polymer includes a plurality of a first monomer including a terminal functional group that is to attach to at least two different primers; a plurality of a second monomer including a second functional group that is different from the terminal functional group, and that is selected from the group consisting of a phenyl group, methoxy propyl, glycosyl, vinyl pyrrolidone, and an imidazole group; and a plurality of a third monomer that is different from the first and second monomers. This co-polymer may be used in a flow cell, and may enhance the clustering efficiency and kinetics.

公开(公告)号：US20220154177A1

公开(公告)日：2022-05-19

申请号：US17525553

申请日：2021-11-12

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Gianluca Andrea Artioli ,  Mathieu Lessard-Viger ,  Brian D. Mather ,  Xavier von Hatten

IPC: C12N15/10 ,  C08F265/10 ,  C08J3/075

Abstract: An example of a functionalized plasmonic nanostructure includes a plasmonic nanostructure core; a polymeric hydrogel attached to the plasmonic nanostructure core, the polymeric hydrogel having a thickness ranging from about 10 nm to about 200 nm; and a plurality of primers attached to side chains or arms of the polymeric hydrogel, wherein at least some of the plurality of primers are attached to the polymeric hydrogel at different distances from the plasmonic nanostructure core.

公开(公告)号：US20240352510A1

公开(公告)日：2024-10-24

申请号：US18633966

申请日：2024-04-12

Applicant: Illumina, Inc.

Inventor： Mathieu Lessard-Viger ,  Rebecca Turk-MacLeod ,  Vanessa Montaño-Machado ,  Jeffrey Fisher ,  Rohit Subramanian ,  Krishnarjun Sarkar ,  Sahngki Hong ,  Weixian Xi ,  Brandon Wenning ,  Lewis Kraft ,  Wayne George ,  Brian Mather ,  Allison Meade ,  John Daly

IPC: C12Q1/6848 ,  C12Q1/6855

CPC classification number: C12Q1/6848 ,  C12Q1/6855

Abstract: In some examples, a structure is contacted with polynucleotides having a variety of lengths and each including first and second adapters. The structure includes a substrate including first and second regions spaced apart from one another by a gap of at least 100 nm, a first set of capture primers coupled to the first region of the substrate, and a second set of capture primers coupled to the second region of the substrate. The first adapter of the polynucleotide is hybridized to a capture primer of the first set of capture primers. Based on that polynucleotide being sufficiently long to bridge the gap, it is amplified using the first and second sets of capture primers. Based upon that polynucleotide being insufficiently long to bridge the gap, it is not amplified. Optionally, a wall may be disposed in the gap.

公开(公告)号：US20230391997A1

公开(公告)日：2023-12-07

申请号：US18233276

申请日：2023-08-11

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Wayne N. George ,  Ludovic Vincent ,  Andrew A. Brown ,  Mathieu Lessard-Viger

IPC: C08L33/08 ,  C12Q1/6869 ,  C12Q1/6853 ,  C08L33/26

CPC classification number: C08L33/08 ,  C08L33/26 ,  C12Q1/6853 ,  C12Q1/6869

Abstract: A flow cell includes a support and a heteropolymer attached to the support. The heteropolymer includes an acrylamide monomer including an attachment group to react with a functional group attached to a primer, and a monomer including a stimuli-responsive functional group. The monomer including the stimuli-responsive functional group may be pH-responsive, temperature-responsive, saccharide-responsive, nucleophile-responsive, and/or salt-responsive.

公开(公告)号：US20230116852A1

公开(公告)日：2023-04-13

申请号：US17813852

申请日：2022-07-20

Applicant: Illumina, Inc.

Inventor： Hayden Black ,  Mathieu Lessard-Viger ,  James Tsay

IPC: C12N15/10 ,  C12Q1/6806

Abstract: Embodiments of the present disclosure relate to method of preparing a substrate for sequencing by synthesis, including capturing library DNA to the surface using a low salt buffer solution prior to grafting primer oligonucleotides. Substrates prepared by the method described herein have increased monoclonality of clusters and sequencing by synthesis using the substrate prepared by the method are also described.

公开(公告)号：US20220195519A1

公开(公告)日：2022-06-23

申请号：US17600526

申请日：2020-12-11

Applicant: ILLUMINA, INC.

Inventor： Jeffrey S. Fisher ,  Tarun Kumar Khurana ,  Mathieu Lessard-Viger ,  Clifford Lee Wang ,  Yir-Shyuan Wu

IPC: C12Q1/6874 ,  G01N1/40

Abstract: In an example, a target material is immobilized on two opposed sequencing surfaces of a flow cell using first and second fluids. The first fluid has a density less than a target material density and the second fluid has a density greater than the target material density; or the second fluid has a density less than the target material density and the first fluid has a density greater than the target material density. The first fluid (including the target material) is introduced into the flow cell, whereby at least some of the target material becomes immobilized by capture sites on one of the sequencing surfaces. The first fluid and non-immobilized target material are removed. The second fluid (including target material) is introduced into the flow cell, whereby at least some of the target material becomes immobilized by capture sites on another of the sequencing surfaces.

公开(公告)号：US20250091042A1

公开(公告)日：2025-03-20

申请号：US18965657

申请日：2024-12-02

Applicant: ILLUMINA, INC.

Inventor： Lewis J. Kraft ,  Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Arnaud Rival ,  Justin Fullerton ,  M. Shane Bowen ,  Hui Han ,  Jeffrey S. Fisher ,  Yasaman Farshchi ,  Mathieu Lessard-Viger

IPC: B01L3/00 ,  B01J19/00 ,  C08L37/00 ,  C12N15/10 ,  C40B40/08 ,  C40B50/18 ,  G01N21/05

Abstract: An example of a flow cell includes a substrate, a plurality of chambers defined on or in the substrate, and a plurality of depressions defined in the substrate and within a perimeter of each of the plurality of chambers. The depressions are separated by interstitial regions. Primers are attached within each of the plurality of depressions, and a capture site is located within each of the plurality of chambers.

公开(公告)号：US20240279733A1

公开(公告)日：2024-08-22

申请号：US18567989

申请日：2022-12-15

Applicant: Illumina, Inc.

Inventor： Fei Shen ,  Mathieu Lessard-Viger ,  Eric Brustad ,  Allison Meade ,  Esteban Armijo ,  Michael Howard ,  Jeffrey Fisher ,  Jonathan Boutell ,  Ramon Saracho ,  Olivia Ghazinejad ,  Seth McDonald ,  Lena Storms ,  Jeffrey Brodin

IPC: C12Q1/6874

CPC classification number: C12Q1/6874

Abstract: The present disclosure is directed to decoupling library capture (template seeding) from cluster generation to optimise both processes. This is achieved by introducing orthogonality between the seeding and clustering primer.

公开(公告)号：US20220154273A1

公开(公告)日：2022-05-19

申请号：US17525530

申请日：2021-11-12

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Gianluca Andrea Artioli ,  Mathieu Lessard-Viger ,  Brian D. Mather ,  Seth M. McDonald ,  Kaitlin M. Pugliese ,  Xavier von Hatten

IPC: C12Q1/6874 ,  B01L3/00

Abstract: An example of an incorporation mix includes a liquid carrier, a complex, and a labeled nucleotide. The complex includes a polymerase and a plasmonic nanostructure linked to the polymerase. The labeled nucleotide includes a nucleotide, a 3′ OH blocking group attached to a sugar of the nucleotide, and a dye label attached to a base of the nucleotide.