公开(公告)号：US20210317444A1

公开(公告)日：2021-10-14

申请号：US16903324

申请日：2020-06-16

Applicant: Inscripta, Inc.

Inventor： Andrea HALWEG-EDWARDS ,  Joshua SHORENSTEIN ,  Andrew GARST ,  Craig STRUBLE ,  Miles GANDER ,  Juhan KIM ,  Bryan LELAND ,  Eileen SPINDLER ,  Paul HARDENBOL

IPC: C12N15/10

Abstract: The present disclosure is drawn to creating cassette designs for nucleic acid-guided nuclease editing. In designing editing cassettes, a set of edit specifications must first be obtained. These edit specifications are taken together with a set of configuration parameters to start a computational pipeline that generates a collection of cassette designs. The process of designing editing cassettes involves the following exemplary steps: 1) creation of a set of candidate cassette designs for each unique edit specification, 2) enumeration of features describing biophysical characteristics of each candidate design, 3) providing each candidate design with a score, and 4) returning a number of scored and rank-ordered candidate cassette designs for each edit specification.

公开(公告)号：US20180230460A1

公开(公告)日：2018-08-16

申请号：US15948793

申请日：2018-04-09

Applicant: The Regents of the University of Colorado, a body corporate ,  Inscripta, Inc.

Inventor： Ryan T. GILL ,  Andrew GARST ,  Tanya Elizabeth Warnecke LIPSCOMB ,  Marcelo Colika BASSALO ,  Ramsey Ibrahim ZEITOUN

IPC: C12N15/10 ,  C12N15/11

CPC classification number: C12N15/1065 ,  C12N15/102 ,  C12N15/1079 ,  C12N15/1082 ,  C12N15/11 ,  C12N2310/20 ,  C12Q2563/179

Abstract: Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US20230183683A1

公开(公告)日：2023-06-15

申请号：US18166868

申请日：2023-02-09

Applicant: Inscripta, Inc.

Inventor： Tian TIAN ,  Eileen SPINDLER ,  Clint DAVIS ,  Charles JOHNSON ,  Andrew GARST

IPC: C12N15/10 ,  C12N15/11 ,  C12N9/22 ,  C12N15/63 ,  C12N15/70

CPC classification number: C12N15/1082 ,  C12N15/111 ,  C12N9/22 ,  C12N15/63 ,  C12N15/70 ,  C12N2310/20 ,  C12N2820/002

Abstract: The present disclosure provides compositions of matter, methods, modules and automated multi-module instrumentation for performing editing of live cells followed by curing of editing and engine vectors from prior rounds of editing, followed by curing of the curing vector.

公开(公告)号：US20220246235A1

公开(公告)日：2022-08-04

申请号：US17726250

申请日：2022-04-21

Applicant: Inscripta, Inc.

Inventor： Andrea HALWEG-EDWARDS ,  Joshua SHORENSTEIN ,  Andrew GARST ,  Craig STRUBLE ,  Miles GANDER ,  Juhan KIM ,  Bryan LELAND ,  Eileen SPINDLER ,  Paul HARDENBOL ,  Aaron BROOKS ,  Eric ABBATE

IPC: G16B20/30 ,  G16B40/20 ,  C12N15/10 ,  G06N3/08

Abstract: The present disclosure is drawn to creating cassette designs for nucleic acid-guided nuclease editing. In designing editing cassettes, a set of edit specifications must first be obtained. These edit specifications are taken together with a set of configuration parameters to start a computational pipeline that generates a collection of cassette designs. The process of designing editing cassettes involves the following exemplary steps: 1) creation of a set of candidate cassette designs for each unique edit specification, 2) enumeration of features describing biophysical characteristics of each candidate design, 3) providing each candidate design with a score, and 4) returning a number of scored and rank-ordered candidate cassette designs for each edit specification.

公开(公告)号：US20180230461A1

公开(公告)日：2018-08-16

申请号：US15948798

申请日：2018-04-09

Applicant: The Regents of the University of Colorado, a body corporate ,  Inscripta, Inc.

Inventor： Ryan T. GILL ,  Andrew GARST ,  Tanya Elizabeth Warnecke LIPSCOMB ,  Marcelo Colika BASSALO ,  Ramsey Ibrahim ZEITOUN

IPC: C12N15/10 ,  C12N15/11

CPC classification number: C12N15/1065 ,  C12N15/102 ,  C12N15/1079 ,  C12N15/1082 ,  C12N15/11 ,  C12N2310/20 ,  C12Q2563/179

Abstract: Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US20190390226A1

公开(公告)日：2019-12-26

申请号：US16548631

申请日：2019-08-22

Applicant: Inscripta, Inc.

Inventor： Ryan T. GILL ,  Andrew GARST ,  Tanya Elizabeth Warnecke LIPSCOMB

IPC: C12N15/90 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N9/22 ,  C12N15/11

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20180371498A1

公开(公告)日：2018-12-27

申请号：US15896444

申请日：2018-02-14

Applicant: Inscripta, Inc.

Inventor： Ryan T. GILL ,  Andrew GARST ,  Tanya Elizabeth Warnecke LIPSCOMB

IPC: C12N15/90 ,  C12N15/11 ,  C12N15/85 ,  C12N15/70 ,  C12N9/22 ,  C12N15/81

CPC classification number: C12N15/902 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/905 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/22

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20210317445A1

公开(公告)日：2021-10-14

申请号：US16945575

申请日：2020-07-31

Applicant: Inscripta, Inc.

Inventor： Andrea HALWEG-EDWARDS ,  Joshua SHORENSTEIN ,  Andrew GARST ,  Craig STRUBLE ,  Miles GANDER ,  Juhan KIM ,  Bryan LELAND ,  Eileen SPINDLER ,  Paul HARDENBOL

IPC: C12N15/10

Abstract: The present disclosure is drawn to creating cassette designs for nucleic acid-guided nuclease editing. In designing editing cassettes, a set of edit specifications must first be obtained. These edit specifications are taken together with a set of configuration parameters to start a computational pipeline that generates a collection of cassette designs. The process of designing editing cassettes involves the following exemplary steps: 1) creation of a set of candidate cassette designs for each unique edit specification, 2) enumeration of features describing biophysical characteristics of each candidate design, 3) providing each candidate design with a score, and 4) returning a number of scored and rank-ordered candidate cassette designs for each edit specification.