公开(公告)号：US10669309B2

公开(公告)日：2020-06-02

申请号：US15677023

申请日：2017-08-15

Applicant: Jiangnan University

Inventor： Xusheng Chen ,  Zhonggui Mao ,  Jianhua Zhang ,  Hongjian Zhang ,  Ke Wang ,  Honggang He

IPC: C07K1/36 ,  B01D61/58 ,  C08G69/10 ,  B01J41/05 ,  B01J39/07 ,  B01D15/36 ,  B01J41/07 ,  B01J39/05 ,  B01D21/26 ,  B01D61/02 ,  B01D61/14 ,  C07K1/18 ,  C07K1/34 ,  C07K14/36 ,  B01D63/06

Abstract: The present invention provides a method for extracting ε-polylysine (ε-PL) and its hydrochloride salt from fermentation broth, which belongs to the field of bio-separation engineering. ε-PL and its hydrochloride salt are produced from fermentation broth through sequential solid-liquid separation, ultrafiltration, two-stage ion exchange, nanofiltration, evaporation concentration and drying techniques. Technologies of membrane filtration and two-stage ion exchange are applied to the preparation of ε-PL and its hydrochloride salt in the present invention, and the invention are characterized by reduced cost, improved automation, and increased product yield and purity, and the method of the present invention would be more suitable for industrial production.

公开(公告)号：US20180127462A1

公开(公告)日：2018-05-10

申请号：US15677023

申请日：2017-08-15

Applicant: Jiangnan University

Inventor： Xusheng Chen ,  Zhonggui Mao ,  Jianhua Zhang ,  Hongjian Zhang ,  Ke Wang ,  Honggang He

IPC: C07K1/36 ,  C07K1/34 ,  C07K1/18 ,  C07K14/36 ,  B01D61/14 ,  B01D61/02 ,  B01D15/36 ,  B01D21/26

CPC classification number: C07K1/36 ,  B01D15/362 ,  B01D15/363 ,  B01D21/262 ,  B01D61/027 ,  B01D61/145 ,  B01D61/58 ,  B01D63/06 ,  B01D2311/04 ,  B01D2311/06 ,  B01D2311/2623 ,  B01J39/05 ,  B01J39/07 ,  B01J41/05 ,  B01J41/07 ,  C07K1/18 ,  C07K1/34 ,  C07K14/36 ,  C08G69/10

Abstract: The present invention provides a method for extracting ε-polylysine (ε-PL) and its hydrochloride salt from fermentation broth, which belongs to the field of bio-separation engineering. ε-PL and its hydrochloride salt are produced from fermentation broth through sequential solid-liquid separation, ultrafiltration, two-stage ion exchange, nanofiltration, evaporation concentration and drying techniques. Technologies of membrane filtration and two-stage ion exchange are applied to the preparation of ε-PL and its hydrochloride salt in the present invention, and the invention are characterized by reduced cost, improved automation, and increased product yield and purity, and the method of the present invention would be more suitable for industrial production.