公开(公告)号：US06534312B1

公开(公告)日：2003-03-18

申请号：US09340798

申请日：1999-06-28

申请人： John W. Shiver ,  Mary Ellen Davies ,  Daniel C. Freed ,  Margaret A. Liu ,  Helen C. Perry

发明人： John W. Shiver ,  Mary Ellen Davies ,  Daniel C. Freed ,  Margaret A. Liu ,  Helen C. Perry

IPC分类号： C12N506

CPC分类号： C07K14/005 ,  A61K2039/51 ,  C07K2319/00 ,  C12N15/67 ,  C12N2740/16122

摘要： Synthetic polynucleotides comprising a DNA sequence encoding a peptide or protein are provided. The DNA sequence of the synthetic polynucleotides comprise codons optimized for expression in a nonhomologous host. The invention is exemplified by synthetic DNA molecules encoding HIV env as well as modifications of HIV env. The codons of the synthetic molecules include the projected host cell's preferred codons. The synthetic molecules provide preferred forms of foreign genetic material. The synthetic molecules may be used as a polynucleotide vaccine which provides immunoprophylaxis against HIV infection through neutralizing antibody and cell-mediated immunity. This invention provides polynucleotides which, when directly introduced into a vertebrate in vivo, including mammals such as primates and humans, induces the expression of encoded proteins within the animal.

公开(公告)号：US06696291B2

公开(公告)日：2004-02-24

申请号：US09974702

申请日：2001-10-09

申请人： John W. Shiver ,  Daniel C. Freed ,  Mary Ellen Davies ,  Margaret A. Liu ,  Helen C. Perry

发明人： John W. Shiver ,  Daniel C. Freed ,  Mary Ellen Davies ,  Margaret A. Liu ,  Helen C. Perry

IPC分类号： C12N506

CPC分类号： C07K14/005 ,  A61K39/00 ,  C07K2319/02 ,  C12N2740/16222 ,  Y02A50/412

摘要： Synthetic DNA molecules encoding HIV gag and modifications of HIV gag are provided. The codons of the synthetic molecules are codons preferred by the projected host cell. The synthetic molecules may be used as a polynucleotide vaccine which provides effective immunoprophylaxis against HIV infection through stimulation of neutralizing antibody and cell-mediated immunity.

公开(公告)号：US20110008369A1

公开(公告)日：2011-01-13

申请号：US12922066

申请日：2009-03-03

申请人： Adam C. Finnefrock ,  Tong-Ming Fu ,  Daniel C. Freed ,  Danilo R. Casimiro ,  Fengsheng Li ,  Aimin Tang

发明人： Adam C. Finnefrock ,  Tong-Ming Fu ,  Daniel C. Freed ,  Danilo R. Casimiro ,  Fengsheng Li ,  Aimin Tang

IPC分类号： A61K39/395 ,  C07K16/18 ,  A61P37/04

CPC分类号： C07K16/2896 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  C07K2319/30

摘要： The present invention features PD-1 binding proteins, a subset of which inhibits binding of PD-L1 to the PD-1 receptor. These binding proteins can be employed to modulate the immune system through the manipulation of the PD-1 signaling pathway, enhancing host immunity to treat infections and cancer.

摘要翻译： 本发明的特征在于PD-1结合蛋白，其一部分抑制PD-L1与PD-1受体的结合。 这些结合蛋白可用于通过操纵PD-1信号传导途径来调节免疫系统，增强宿主免疫治疗感染和癌症。

公开(公告)号：US08168757B2

公开(公告)日：2012-05-01

申请号：US12922066

申请日：2009-03-03

申请人： Adam C. Finnefrock ,  Tong-Ming Fu ,  Daniel C. Freed ,  Danilo R. Casimiro ,  Fengsheng Li ,  Aimin Tang

发明人： Adam C. Finnefrock ,  Tong-Ming Fu ,  Daniel C. Freed ,  Danilo R. Casimiro ,  Fengsheng Li ,  Aimin Tang

IPC分类号： C07K16/00

CPC分类号： C07K16/2896 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  C07K2319/30

摘要： The present invention features PD-1 binding proteins, a subset of which inhibits binding of PD-L1 to the PD-1 receptor. These binding proteins can be employed to modulate the immune system through the manipulation of the PD-1 signaling pathway, enhancing host immunity to treat infections and cancer.

摘要翻译： 本发明的特征在于PD-1结合蛋白，其一部分抑制PD-L1与PD-1受体的结合。 这些结合蛋白可用于通过操纵PD-1信号传导途径来调节免疫系统，增强宿主免疫治疗感染和癌症。

公开(公告)号：US20110136896A1

公开(公告)日：2011-06-09

申请号：US13056899

申请日：2009-07-28

申请人： Tong-Ming Fu ,  Danilo R. Casimiro ,  Daniel C. Freed ,  Aimin Tang

发明人： Tong-Ming Fu ,  Danilo R. Casimiro ,  Daniel C. Freed ,  Aimin Tang

IPC分类号： A61K31/7088 ,  C07H21/04 ,  C07K14/005 ,  C12N15/63 ,  C12P21/06

CPC分类号： C07K14/005 ,  C12N2710/16122

摘要： The present invention relates to compositions and methods to elicit or enhance cell-mediated immunity against HCMV infection by providing polynucleotides encoding variant HCMV pp65, IE1, and IE2 proteins, and fusion proteins thereof. The present invention also provides recombinant vectors including, but not limited to, adenovirus and plasmid vectors comprising said polynucleotides and host cells comprising said recombinant vectors. Also provided herein are purified forms of the variant HCMV pp65, IE1, and IE2 proteins described herein, and fusion proteins. The variant HCMV proteins, and fusion proteins thereof, are useful as vaccines for the protection from and/or treatment of HCMV infection. Said vaccines are useful as a monotherapy or a part of a therapeutic regime, said regime comprising administration of a second vaccine such as a polynucleotide, cell-based, protein or peptide-based vaccine.

摘要翻译： 本发明涉及通过提供编码变体HCMV pp65，IE1和IE2蛋白的多核苷酸及其融合蛋白来引发或增强细胞介导的HCMV感染免疫的组合物和方法。 本发明还提供重组载体，包括但不限于腺病毒和包含所述多核苷酸的质粒载体和包含所述重组载体的宿主细胞。 本文还提供了本文所述的变体HCMV pp65，IE1和IE2蛋白的纯化形式，以及融合蛋白。 变体HCMV蛋白及其融合蛋白可用作用于保护和/或治疗HCMV感染的疫苗。 所述疫苗可用作单一疗法或治疗方案的一部分，所述方案包括施用第二种疫苗，例如多核苷酸，基于细胞的，蛋白质或基于肽的疫苗。