公开(公告)号：US20160186236A1

公开(公告)日：2016-06-30

申请号：US14894985

申请日：2014-05-30

Applicant: K-MAC

Inventor： Lee-Kyung KIM ,  Mun-Cheol PAEK ,  Su-Jin KU ,  Sun-Young PARK ,  Do-Bu LEE ,  Jae-Hyung PARK ,  Ki-Chang LEE ,  Nam-Joong KIM

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6818 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q2537/101 ,  C12Q2561/113 ,  C12Q2565/501

Abstract: A method of quantitatively and qualitatively analyzing a biomaterial in real-time, the method comprising preparing a device for detecting a biomaterial, feeding a complex of first and second probes, a forward primer, a reverse primer, a sample comprising deoxynucleotide triphosphate, a polymerase having exonuclease activity, and a sample comprising target genes, and a reaction solution comprising a buffer into the reaction container, performing polymerase chain reaction comprising denaturation of the target genes in the sample, hybridization of the target genes, the complex, and the forward and reverse primers in the sample, and elongation of the primers through the polymerase having exonuclease activity, allowing for elongation of the second probe on the third probe by the polymerase after hybridizing the released second probe and the third probe fixed to the biochip, detecting a first fluorescence signal by the first phosphor and a second fluorescence signal by the second phosphor.

Abstract translation: 一种实时定量分析生物材料的方法，所述方法包括制备用于检测生物材料的装置，进料第一和第二探针的复合物，正向引物，反向引物，包含脱氧核苷酸三磷酸的样品，聚合酶 具有核酸外切酶活性的试剂盒和包含靶基因的样品和包含缓冲液的反应溶液加入到反应容器中，进行聚合酶链式反应，包括样品中靶基因的变性，靶基因的杂交， 样品中的反向引物和通过具有核酸外切酶活性的聚合酶的引物的延伸，允许在将释放的第二探针和固定到生物芯片上的第三探针杂交后，通过聚合酶在第三探针上延伸第二探针，检测第一 由第一荧光体产生的荧光信号和由第二荧光体产生的第二荧光信号。