公开(公告)号：US10087428B2

公开(公告)日：2018-10-02

申请号：US14646895

申请日：2013-12-20

Applicant: Kao Corporation

Inventor： Tatsuro Ozaki ,  Hiroyuki Ohta ,  Koichi Hori

IPC: C12P7/64 ,  C12N1/00 ,  C12N1/13 ,  C12N1/21 ,  C12N9/16

Abstract: An acyl-ACP thioesterase consisting of an amino acid sequence of the 115th to 274th amino acids set forth in SEQ ID NO: 1; an acyl-ACP thioesterase gene encoding the protein; a transformant having the gene; and a method of producing a lipid using the transformant.

公开(公告)号：US10597646B2

公开(公告)日：2020-03-24

申请号：US16113163

申请日：2018-08-27

Applicant: Kao Corporation

Inventor： Tatsuro Ozaki ,  Hiroyuki Ohta ,  Koichi Hori

IPC: C12P7/64 ,  C12N1/00 ,  C12N1/13 ,  C12N9/16 ,  C12N1/21

Abstract: An acyl-ACP thioesterase consisting of an amino acid sequence of the 115th to 274th amino acids set forth in SEQ ID NO: 1; an acyl-ACP thioesterase gene encoding the protein; a transformant having the gene; and a method of producing a lipid using the transformant.

公开(公告)号：US10066248B2

公开(公告)日：2018-09-04

申请号：US15110635

申请日：2015-02-23

Applicant: Kao Corporation

Inventor： Shinji Sugihara ,  Tatsuro Ozaki ,  Hiroko Endo ,  Takeshi Saito ,  Takuto Tojo ,  Hiroyuki Ohta ,  Koichi Hori

IPC: C12P7/64 ,  C12N1/13 ,  C12N9/88 ,  C12N9/10 ,  C12N9/16 ,  C12N15/52 ,  C12N15/70 ,  C12N15/79

CPC classification number: C12P7/6436 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/88 ,  C12N15/52 ,  C12N15/70 ,  C12N15/79 ,  C12P7/64 ,  C12P7/6409 ,  C12Y203/01041 ,  C12Y301/02014

Abstract: The present disclosure provides a gene encoding a protein having β-ketoacyl-ACP synthase activity having at least 60% or more identity with the amino acid sequence set forth in SEQ ID NO:1, the protein encoded by the gene, a method of producing a transformant by transforming a host with the gene, a transformant that is transformed with the gene, and a method of producing a lipid, especially, a lipid containing a medium chain fatty acid or ester thereof, by culturing the transformed host that expresses the gene. In some embodiments, the transformant also has a gene encoding an acyl-ACP thioesterase.

公开(公告)号：US20180371436A1

公开(公告)日：2018-12-27

申请号：US16113163

申请日：2018-08-27

Applicant: Kao Corporation

Inventor： Tatsuro Ozaki ,  Hiroyuki Ohta ,  Koichi Hori

IPC: C12N9/16 ,  C12P7/64

CPC classification number: C12N9/16 ,  C12P7/6409 ,  C12Y301/02014

Abstract: An acyl-ACP thioesterase consisting of an amino acid sequence of the 115th to 274th amino acids set forth in SEQ ID NO: 1; an acyl-ACP thioesterase gene encoding the protein; a transformant having the gene; and a method of producing a lipid using the transformant.