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公开(公告)号：US06777190B1
公开(公告)日：2004-08-17
申请号：US08241061
申请日：1994-05-11
IPC分类号： A61K39395
CPC分类号： C07D207/416 , C12N9/16 , G01N33/5306 , G01N33/54353 , G01N33/94
摘要： Assay for detecting the amount or presence of target ligand in a sample. The assay includes a ligand analogue conjugate having a linkage site and a binding site, a ligand receptor, and a sample. The assay includes the steps of providing at least one crosstalk inhibitor. This inhibitor, under assay conditions, competes with the linkage site of the ligand analogue conjugate for binding to the ligand receptor, and does not compete with the binding site of the ligand analogue conjugate for binding to the ligand receptor. In the invention, the assay is performed for the target ligand in the presence of a sufficient amount of the crosstalk inhibitor to reduce the amount of binding of the linkage site of the ligand analogue conjugate to the ligand receptor. The invention also features a method for identifying crosstalk inhibitors, and the crosstalk inhibitors themselves.
摘要翻译： 用于检测样品中靶配体的量或存在的测定。 该测定包括具有连接位点和结合位点，配体受体和样品的配体类似物缀合物。 该测定包括提供至少一种串扰抑制剂的步骤。 在测定条件下，该抑制剂与配体类似物缀合物的连接位点竞争结合配体受体，并且不与配体类似物缀合物的结合位点竞争结合配体受体。 在本发明中，在足够量的串扰抑制剂的存在下，对靶配体进行测定以减少配体类似物缀合物与配体受体的连接位点的结合量。 本发明还具有用于识别串扰抑制剂和串扰抑制剂本身的方法。
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公开(公告)号：US5525524A
公开(公告)日：1996-06-11
申请号：US101677
申请日：1993-08-03
IPC分类号： C07D207/40 , C07D207/416 , C07K14/00 , C07K14/76 , C12N9/16 , G01N33/53 , G01N33/531 , G01N33/543 , G01N33/94 , G01N33/74
CPC分类号： C07D207/416 , C12N9/16 , G01N33/5306 , G01N33/54353 , G01N33/94 , Y10S435/962 , Y10S436/816 , Y10S436/817 , Y10S436/822
摘要： Assay for detecting the amount or presence of target ligand in a sample. The assay includes a ligand analogue conjugate having a linkage site and a binding site, a ligand receptor, and a sample. The assay includes the steps of providing at least one crosstalk inhibitor. This inhibitor, under assay conditions, competes with the linkage site of the ligand analogue conjugate for binding to the ligand receptor, and does not compete with the binding site of the ligand analogue conjugate for binding to the ligand receptor. In the invention, the assay is performed for the target ligand in the presence of a sufficient amount of the crosstalk inhibitor to reduce the amount of binding of the linkage site of the ligand analogue conjugate to the ligand receptor. The invention also features a method for identifying crosstalk inhibitors, and the crosstalk inhibitors themselves.
摘要翻译： 用于检测样品中靶配体的量或存在的测定。 该测定包括具有连接位点和结合位点，配体受体和样品的配体类似物缀合物。 该测定包括提供至少一种串扰抑制剂的步骤。 在测定条件下，该抑制剂与配体类似物缀合物的连接位点竞争结合配体受体，并且不与配体类似物缀合物的结合位点竞争结合配体受体。 在本发明中，在足够量的串扰抑制剂的存在下，对靶配体进行测定以减少配体类似物缀合物与配体受体的连接位点的结合量。 本发明还具有用于识别串扰抑制剂和串扰抑制剂本身的方法。
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公开(公告)号：US5679526A
公开(公告)日：1997-10-21
申请号：US284035
申请日：1994-08-01
IPC分类号： G01N33/53 , G01N33/543 , G01N33/58 , G01N33/74 , G01N33/94
CPC分类号： G01N33/94 , G01N33/53 , G01N33/54306 , G01N33/58 , G01N33/581 , G01N33/585 , G01N33/743 , Y10S435/805 , Y10S435/81 , Y10S435/967 , Y10S436/807 , Y10S436/81 , Y10S436/825
摘要： This invention is directed to a ligand-receptor assay for determining the presence of at least one target ligand, capable of competing with a ligand analogue conjugate for binding sites available on a ligand receptor, said ligand analogue conjugate comprising at least one ligand analogue coupled to a colloidal gold particle, in a fluid sample suspected of containing said target ligand comprising the steps of: a. contacting said fluid sample with said ligand analogue conjugate and said ligand receptor to form a homogeneous reaction mixture, the relative amounts of said ligand analogue conjugate and said ligand receptor being selected such that in the absence of said target ligand and subsequent to substantially equilibrium binding in said reaction mixture, substantially all of said ligand analogue conjugate is bound to said ligand receptor such that no unbound ligand analogue conjugate is detected as a result of the assay method; b. detecting unbound ligand analogue conjugates in said reaction mixture by visual means using at least one ligand receptor immobilized on a solid phase in at least one distinct locus; c. relating the detectable signal to the presence of said target ligand in said fluid sample.
摘要翻译： 本发明涉及用于测定能够与配体受体上可获得的结合位点与配体类似物缀合物竞争的至少一种靶配体的存在的配体 - 受体测定，所述配体类似物缀合物包含至少一种与 在怀疑含有所述靶配体的流体样品中的胶态金颗粒包括以下步骤：a。 使所述流体样品与所述配体类似物缀合物和所述配体受体接触以形成均匀的反应混合物，所述配体类似物缀合物和所述配体受体的相对量被选择为使得在不存在所述靶配体的情况下并且随后基本上平衡地结合 所述反应混合物，基本上所有的所述配体类似物缀合物都与所述配体受体结合，使得作为测定方法的结果没有检测到未结合的配体类似物缀合物; b。 通过视觉方式在所述反应混合物中检测未结合的配体类似物缀合物，使用固定在至少一个不同基因座的固相上的至少一种配体受体; C。 将可检测信号与所述流体样品中所述目标配体的存在相关联。
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公开(公告)号：US5939272A
公开(公告)日：1999-08-17
申请号：US871900
申请日：1997-06-11
IPC分类号： G01N33/53 , G01N33/543 , G01N33/566 , G01N33/58 , G01N33/74 , G01N33/94 , G01N33/558
CPC分类号： G01N33/54306 , G01N33/53 , G01N33/566 , G01N33/58 , G01N33/581 , G01N33/585 , G01N33/743 , G01N2333/805 , G01N33/94 , Y10S435/967 , Y10S436/807
摘要： Assay methods for performing non-competitive ligand-receptor assays, where said assays provide a sensible result when the target ligand identified by the assay is present at a concentration greater than a defined threshold concentration. The threshold concentration can be selected to be the upper limit of a normal range of values for a target ligand in a sample, so that an assay in accordance with the invention provides a sensible result only when the target ligand is present at elevated levels.
摘要翻译： 用于进行非竞争性配体受体测定的测定方法，其中当通过测定法鉴定的靶配体以大于所定义的阈值浓度的浓度存在时，所述测定提供了明智的结果。 阈值浓度可以选择为样品中靶配体的正常范围值的上限，使得根据本发明的测定仅在靶配体存在于升高的水平时提供显着的结果。
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5.发明授权Assay devices comprising a porous capture membrane in fluid-withdrawing contact with a nonabsorbent capillary network 失效测定装置包括与非吸收性毛细管网流体吸收接触的多孔捕获膜
公开(公告)号：US5922615A
公开(公告)日：1999-07-13
申请号：US458276
申请日：1995-06-02
IPC分类号： G01N33/53 , G01N33/543 , G01N33/558 , G01N37/00
CPC分类号： G01N33/558 , G01N33/5302 , G01N33/54366 , Y10S435/805 , Y10S435/81 , Y10S435/97 , Y10S435/975 , Y10S436/805 , Y10S436/81
摘要： Devices for use in heterogeneous ligand-receptor assays, having a porous member in contact with a nonabsorbent textured surface, where the surface texturing is such that a capillary network is formed when in fluid communication with the porous member. More particularly, these devices comprise:(a) a porous member having (i) at least one binding agent capable of immobilizing at least one target ligand on the porous member from a fluid sample in at least one zone and (ii) a means for detecting the presence or amount of said target ligand as a result of the assay process; and(b) a nonabsorbent member in fluid communication with the porous member, the nonabsorbent member forming at least one capillary with the porous member so that when sample, alone or in combination with other fluids, is added to the porous member, fluid is drawn through the porous member.
摘要翻译： 用于异构配体 - 受体测定的装置，其具有与非吸收性织构化表面接触的多孔构件，其中表面纹理使得当与多孔构件流体连通时形成毛细管网。 更具体地说，这些装置包括：（a）多孔构件，其具有（i）能够在至少一个区域中从流体样品将至少一种目标配体固定在多孔构件上的至少一种结合剂，和（ii） 检测作为测定过程的结果的所述目标配体的存在或量; 和（b）与所述多孔构件流体连通的非吸收构件，所述非吸收构件与所述多孔构件形成至少一个毛细管，使得当将单独或与其它流体组合的样品加入到所述多孔构件中时，绘制流体 通过多孔构件。
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公开(公告)号：US5089391A
公开(公告)日：1992-02-18
申请号：US463150
申请日：1990-01-10
IPC分类号： G01N33/53 , G01N33/543 , G01N33/58 , G01N33/74 , G01N33/94
CPC分类号： G01N33/94 , G01N33/53 , G01N33/54306 , G01N33/58 , G01N33/581 , G01N33/585 , G01N33/743 , Y10S435/805 , Y10S435/81 , Y10S435/967 , Y10S436/807 , Y10S436/81 , Y10S436/825
摘要： This invention is directed to a ligand-receptor assay for determining the presence or amount of at least one target ligand, capable of competing with a ligand analogue conjugate for binding sites available on a ligand receptor, said ligand analogue conjugate comprising at least one ligand analogue coupled to a signal development element capable of emitting a detectable signal, in a fluid sample suspected of containing said target ligand, comprising the steps of:a. contacting said fluid sample with ligand analogue conjugate and ligand receptor to form a reaction mixture, the relative amounts of ligand analogue conjugate and ligand receptor being such that in the absence of target ligand, and subsequent to substantially equilibrium binding, substantially all of the ligand analogue conjugate is bound to ligand receptor;b. detecting the unbound ligand analogue conjugate;c. relating the detectable signal to the presence or amount of target ligand in the fluid sample. In one embodiment an optional means also is employed for removing receptor from the reaction mixture. In related claimed assay formats the analyte of interest may be either ligand receptor or ligand.
摘要翻译： 本发明涉及用于确定能够与配体受体上可用的结合位点与配体类似物缀合物竞争的至少一种靶配体的存在或量的配体 - 受体测定，所述配体类似物缀合物包含至少一种配体类似物 耦合到在怀疑含有所述靶配体的流体样品中能够发射可检测信号的信号发育元件，其包括以下步骤：a。 使所述流体样品与配体类似物缀合物和配体受体接触以形成反应混合物，配体类似物缀合物和配体受体的相对量使得在不存在靶配体的情况下，并且在基本平衡结合之后，基本上所有的配体类似物 缀合物与配体受体结合; b。 检测未结合的配体类似物缀合物; C。 将可检测信号与流体样品中靶配体的存在或量相关联。 在一个实施方案中，也可以使用任选的方法从反应混合物中除去受体。 在相关要求的测定形式中，感兴趣的分析物可以是配体受体或配体。
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公开(公告)号：US5028535A
公开(公告)日：1991-07-02
申请号：US295568
申请日：1989-01-10
IPC分类号： G01N33/53 , G01N33/543 , G01N33/58 , G01N33/74 , G01N33/94
CPC分类号： G01N33/94 , G01N33/53 , G01N33/54306 , G01N33/58 , G01N33/581 , G01N33/585 , G01N33/743 , Y10S435/805 , Y10S435/81 , Y10S435/967 , Y10S436/807 , Y10S436/81 , Y10S436/825
摘要： This invention is directed to a ligand-receptor assay for determining the presence or amount of at least one target ligand, capable of competing with a ligand analogue conjugate for binding sites available on a ligand receptor, said ligand analogue conjugate comprising at least one ligand analogue coupled to a signal development element capable of emitting a detectable signal, in a fluid sample suspected of containing said target ligand, comprising the steps of:a. contacting said fluid sample with ligand analogue conjugate and ligand receptor to form a reaction mixture, the relative amounts of ligand analogue conjugate and ligand receptor being such that in the absence of target ligand, and subsequent to substantially equilibrium binding, substantially all of the ligand analogue conjugate is bound to ligand receptor;b. detecting the unbound ligand analogue conjugate;c. relating the detectable signal to the presence or amount of target ligand in the fluid sample. In one embodiment an optional means also is employed for removing receptor from the reaction mixture. In related claimed assay formats the analyte of interest may be either ligand receptor or ligand.
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8.发明授权Assay devices comprising a porous capture membrane in fluid-withdrawing contact with a nonabsorbent capillary network 有权测定装置包括与非吸收性毛细管网流体吸收接触的多孔捕获膜公开(公告)号：US06297060B1
公开(公告)日：2001-10-02
申请号：US09249321
申请日：1999-02-11
IPC分类号： G01N33543
CPC分类号： G01N33/558 , G01N33/5302 , G01N33/54366 , Y10S435/805 , Y10S435/81 , Y10S435/97 , Y10S435/975 , Y10S436/805 , Y10S436/81
摘要： Devices for use in heterogeneous ligand-receptor assays, having a porous member in contact with a nonabsorbent textured surface, where the surface texturing is such that a capillary network is formed when in fluid communication with the porous member. More particularly, these devices comprise: (a) a porous member having (i) at least one binding agent capable of immobilizing at least one target ligand on the porous member from a fluid sample in at least one zone and (ii) a means for detecting the presence or amount of said target ligand as a result of the assay process; and (b) a nonabsorbent member in fluid communication with the porous member, the nonabsorbent member forming at least one capillary with the porous member so that when sample, alone or in combination with other fluids, is added to the porous member, fluid is drawn through the porous member.
摘要翻译： 用于异构配体 - 受体测定的装置，其具有与非吸收性织构化表面接触的多孔构件，其中表面纹理使得当与多孔构件流体连通时形成毛细管网。 更具体地说，这些装置包括：（a）多孔构件，其具有（i）能够在至少一个区域中从流体样品将至少一种目标配体固定在多孔构件上的至少一种结合剂，和（ii） 检测作为测定过程的结果的所述目标配体的存在或量; 和（b）与所述多孔构件流体连通的非吸收构件，所述非吸收构件与所述多孔构件形成至少一个毛细管，使得当将单独或与其它流体组合的样品加入到所述多孔构件中时，绘制流体 通过多孔构件。
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9.发明授权
公开(公告)号：US5480792A
公开(公告)日：1996-01-02
申请号：US71203
申请日：1993-06-01
IPC分类号： G01N33/53 , G01N33/538 , G01N33/541 , G01N33/543 , G01N33/564 , C12Q1/68 , G01N33/531 , G01N33/577
CPC分类号： G01N33/54306 , G01N33/5306 , G01N33/541 , G01N33/54393 , Y10S435/97 , Y10S435/971 , Y10S435/975 , Y10S436/807
摘要： Methods and test devices for detecting the presence or amount of target ligand in non-competitive sandwich ligand-receptor assay processes. Antibodies which bind to the complex of ligand receptor and target ligand but do not bind significantly to the ligand receptor and which bind the target ligand with substantially less affinity than the complex are taught and their uses described. These assays can be used to eliminate the "hook" effect in non-competitive sandwich assays. Furthermore, the antibodies are selected and assay methods described so that, as a result of the assay process, no detectable response is observed due to the binding of antibody and ligand receptor in the absence of target ligand.
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10.发明授权公开(公告)号：US07608406B2
公开(公告)日：2009-10-27
申请号：US10875800
申请日：2004-06-23
IPC分类号： G01N33/53
CPC分类号： G01N33/6896 , G01N2800/2871
摘要： The present invention relates to methods for the diagnosis and evaluation of stroke and transient ischemic attacks. A variety of markers are disclosed for assembling a panel for such diagnosis and evaluation. In various aspects, the invention provides methods for early detection and differentiation of stroke types and transient ischemic attacks, for determining the prognosis of a patient presenting with stroke symptoms, and identifying a patient at risk for cerebral vasospasm. Invention methods provide rapid, sensitive and specific assays to greatly increase the number of patients that can receive beneficial stroke treatment and therapy, and reduce the costs associated with incorrect stroke diagnosis.
摘要翻译： 本发明涉及中风和短暂性脑缺血发作的诊断和评估方法。 公开了用于组装用于这种诊断和评估的面板的各种标记。 在各个方面，本发明提供了用于早期检测和分化卒中类型和短暂性脑缺血发作的方法，用于确定患有中风症状的患者的预后，以及鉴定患有脑血管痉挛风险的患者。 本发明方法提供快速，灵敏和特异性的测定以大大增加可以接受有益的中风治疗和治疗的患者的数量，并且降低与不正确的中风诊断相关的成本。
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