公开(公告)号：US6124429A

公开(公告)日：2000-09-26

申请号：US752526

申请日：1996-11-20

申请人： Kin-ichiro Miura ,  Shuichi Kojima ,  Yukino Kuriki ,  Takao Yoshida ,  Susumu Nakae ,  Yoko Doi

发明人： Kin-ichiro Miura ,  Shuichi Kojima ,  Yukino Kuriki ,  Takao Yoshida ,  Susumu Nakae ,  Yoko Doi

IPC分类号： C12N15/09 ,  A61L27/00 ,  C07H21/04 ,  C07K7/06 ,  C07K14/00 ,  C12P21/02 ,  C12R1/19 ,  G03C5/00

CPC分类号： C07K14/00

摘要： A polypeptide having .alpha.-helix structure, which has a hydrophilic surface and a hydrophobic surface, and which is produced by genetic engineering techniques is disclosed. According to the present invention, stable polypetide molecules having .alpha.-helix structure, which have a constant molecular weight and which can be used in various applications represented by materials for medical applications, can be provided.

摘要翻译： 公开了具有亲水表面和疏水性表面的α-螺旋结构的多肽，其通过遗传工程技术生产。 根据本发明，可以提供具有恒定分子量并可用于医疗用材料的各种应用中的具有α-螺旋结构的稳定的多肽分子。

公开(公告)号：US4855237A

公开(公告)日：1989-08-08

申请号：US643036

申请日：1984-08-21

申请人： Tsuto Morinaga ,  Kin-ichiro Miura ,  Kunitada Shimotohno ,  Masato Ikegami ,  Yataro Ichikawa

发明人： Tsuto Morinaga ,  Kin-ichiro Miura ,  Kunitada Shimotohno ,  Masato Ikegami ,  Yataro Ichikawa

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N15/75 ,  C12N15/81 ,  C12N15/82

CPC分类号： C12N15/81 ,  C12N15/75 ,  C12N15/8203 ,  Y10S435/948

摘要： Double-stranded DNA characterized by having sequences complementary to a single-stranded DNA which has a molecular size of about 2.67 Kb and is derived from mungbean yellow mosaic virus, and giving the restriction endonuclease cleavage map shown in FIG. 1 of the accompanying drawings; and double-stranded DNA characterized by having sequences complementary to a single-stranded DNA which has a molecular size of about 2.70 Kb and is derived from mungbean yellow mosaic virus, and giving the restriction endonuclease cleavage map shown in FIG. 2 of the accompanying drawings; and hybrid DNAs having the double-stranded DNAs inserted thereinto.

摘要翻译： 其特征在于具有与分子大小约2.67Kb并衍生自绿豆黄花叶病毒的单链DNA互补的序列，并给出图1所示的限制性内切酶切割图。 附图1; 特征在于具有与分子大小约2.70Kb并衍生自绿豆黄花叶病毒的单链DNA互补的序列的双链DNA，并给出图1所示的限制性内切酶切割图。 附图2; 以及插入双链DNA的杂交DNA。

公开(公告)号：US06495675B1

公开(公告)日：2002-12-17

申请号：US09603378

申请日：2000-06-26

申请人： Hiroshi Takaku ,  Kin-ichiro Miura ,  Toshifumi Hatta ,  Kazuyuki Takai ,  Masahide Ishikawa

发明人： Hiroshi Takaku ,  Kin-ichiro Miura ,  Toshifumi Hatta ,  Kazuyuki Takai ,  Masahide Ishikawa

IPC分类号： C07H2104

CPC分类号： A61K31/7105 ,  A61K38/00 ,  C07H21/00 ,  C12N15/1131 ,  C12N2310/317 ,  C12N2310/321 ,  C12N2310/3521

摘要： A pharmaceutical composition comprising a capped oligonucleotide capable of binding to a PB2 protein of an influenza virus RNA polymerase, and a pharmaceutically acceptable carrier or dilute, said capped oligonucleotide having a structure of the formula (I): m7GpppXpY  (I) wherein ppp is a triphosphate bridge, p is a monophosphate bridge, m7G is 7-methylguanosine group binding at 5′ end thereof to said ppp, X is a 2′-O-methyl guanosine group or guanosine group, said 2′-O-methyl guanosine group or guanosine group binds at a 5′ end thereof to said ppp, and at a 3′ end thereof to said p, and Y is an oligoribonucleotide moiety binding at 5′ end thereof to said p, and having 5 to 11 bases is disclosed. The pharmaceutical composition can inhibit a protein expression of an influenza virus.

摘要翻译： 一种药物组合物，其包含能够结合流感病毒RNA聚合酶的PB2蛋白的封端寡核苷酸和药学上可接受的载体或稀释的具有式（I）结构的所述封端寡核苷酸：其中ppp是三磷酸桥，p 是单磷酸桥，m7G是在5'末端与所述ppp结合的7-甲基鸟苷类，X是2'-O-甲基鸟苷或鸟苷酸，所述2'-O-甲基鸟苷或鸟苷基结合于 其5'端至所述ppp，并且在其3'末端与所述p结合，Y是在其5'末端与所述p结合并且具有5至11个碱基的寡核糖核苷酸部分。 药物组合物可以抑制流感病毒的蛋白质表达。

公开(公告)号：US4789636A

公开(公告)日：1988-12-06

申请号：US647829

申请日：1984-09-05

申请人： Tsuto Morinaga ,  Kin-ichiro Miura ,  Kunitada Shimotohno ,  Masato Ikegami ,  Yataro Ichikawa

发明人： Tsuto Morinaga ,  Kin-ichiro Miura ,  Kunitada Shimotohno ,  Masato Ikegami ,  Yataro Ichikawa

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N15/75 ,  C12N15/81 ,  C12N15/82 ,  C12N15/00 ,  C12P19/34 ,  C12P21/00

CPC分类号： C12N15/75 ,  C12N15/81 ,  C12N15/8203

摘要： Double-stranded DNA characterized by having sequences complementary to a single-stranded DNA which has a molecular size of about 2.67 Kb and is derived from mungbean yellow mosaic virus, and giving the restriction endonuclease cleavage map shown in FIG. 1 of the accompanying drawings; anddouble-stranded DNA characterized by having sequences complementary to a single-straned DNA which has a molecular size of about 2.70 Kb and is derived from mungbean yellow mosaic virus, and giving the restriction endonuclease cleavage map shown in FIG. 2 of the accompanying drawings; and hybrid DNAs having the double-stranded DNAs inserted thereinto.

摘要翻译： 其特征在于具有与分子大小约2.67Kb并衍生自绿豆黄花叶病毒的单链DNA互补的序列，并给出图1所示的限制性内切核酸酶切割图。 附图1; 特征在于具有与分子大小约2.70Kb并衍生自绿豆黄色花叶病毒的单链DNA的序列互补的双链DNA，并给出图1所示的限制性内切核酸酶切割图。 附图2; 以及插入双链DNA的杂交DNA。