公开(公告)号：US20230076299A1

公开(公告)日：2023-03-09

申请号：US17779210

申请日：2020-11-16

申请人： Kiweon CHA ,  Korea Institute of Ocean Science & Technology

发明人： Jung-Hyun LEE ,  Hyung-Soon YIM ,  Young Jun AN ,  Kyeon Won LEE ,  Ye Eun JEONG ,  Kiweon CHA ,  Won Kyu LEE ,  Jurang WOO

IPC分类号： C07K14/50 ,  C12N5/0735

摘要： Provided is a polypeptide having FGF2 activity and improved temperature stability and protease resistance. The polypeptide includes at least one substitution selected from a substitution of aspartic acid (D) with glutamic acid (E) at position 28, a substitution of cysteine (C) with isoleucine (I) or leucine (L) at position 78, or a substitution of cysteine (C) with isoleucine (I) or tryptophan (VV) at position 96 in SEQ ID NO: 1.

公开(公告)号：US20220213463A1

公开(公告)日：2022-07-07

申请号：US17292967

申请日：2018-11-28

申请人： KOREA INSTITUTE OF OCEAN SCIENCE & TECHNOLOGY

发明人： Jung-Hyun LEE ,  Jae Kyu LIM ,  Hyun Sook LEE ,  Sung Gyun KANG ,  Kae Kyoung KWON ,  Yun Jae KIM ,  Ji-in YANG

IPC分类号： C12N9/96 ,  C12N9/02 ,  C12N15/52 ,  C12P7/40

摘要： The present invention relates to an Fe—S fusion protein acting as an electron transport chain, a novel carbon monoxide:formate oxidoreductase (CFOR) including the Fe—S fusion protein, novel Thermococcus strain BCF12 transformed with CFOR, and the use thereof. According to the present invention, two different enzymes may be physically linked directly to each other through the Fe—S fusion protein of the present invention, and thus electrons generated from any one enzyme may be transported directly to another enzyme through the Fe—S cluster of the Fe—S fusion protein. Accordingly, a reaction that produces a target substance with high efficiency by directly supplying electrons necessary for the production of the target substance is possible without leakage of electrons generated in any one enzyme. In addition, the present invention has an advantage in that the overall enzyme reaction rate and yield can be dramatically improved using a new electron transport reaction. Furthermore, it is possible to ensure the stability of each enzyme by allowing the enzymes to exist in a physically fixed state in cells.

公开(公告)号：US20230287073A1

公开(公告)日：2023-09-14

申请号：US18147796

申请日：2022-12-29

申请人： Korea Institute of Ocean Science & Technology

发明人： Jung-Hyun LEE ,  Hyung-Soon YIM ,  Young Jun AN ,  Kyeong Won LEE ,  Ye Eun JEONG ,  Kiweon CHA ,  Won Kyu LEE

IPC分类号： C07K14/50

CPC分类号： C07K14/50

摘要： A thermally stable polypeptide having FGF7 activity is provided. The polypeptide is a thermally stable polypeptide having FGF7 activity, wherein, in SEQ ID NO: 1, a 120th alanine (A) is substituted with cysteine (C), one including at least one substitution selected from substitution of a 126th lysine (K) with aspartic acid (D) and a 178th lysine (K) with glutamic acid (E) or aspartic acid (D) forms a salt bridge with a 175th arginine (R), and a 133rd cysteine (C) and a 137th cysteine (C) are disulfide bonded.