公开(公告)号：US20160017383A1

公开(公告)日：2016-01-21

申请号：US14874385

申请日：2015-10-03

Applicant: Korea Advanced Institute of Science and Technology

Inventor： Sang Yup Lee ,  Sung Won Lim ,  Hyohak Song

IPC: C12P7/46 ,  C12N1/20

CPC classification number: C12P7/46 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1217 ,  C12N15/902 ,  C12Y203/01054 ,  Y02P20/52

Abstract: The present invention relates to a mutant microorganism, which is selected from the group consisting of genus Mannheimia, genus Actinobacillus and genus Anaerobiospirillum, producing homo-succinic acid and a method for producing homo-succinic acid using the same, and more particularly to a mutant microorganism producing succinic acid at a high concentration while producing little or no other organic acids in anaerobic conditions, which is obtained by disrupting a gene encoding lactate dehydrogenase (ldhA), a gene encoding phosphotransacetylase (pta), and a gene encoding acetate kinase (ackA), without disrupting a gene encoding pyruvate formate lyase (pfl), as well as a method for producing succinic acid using the same. The inventive mutant microorganism has the property of having a high growth rate and succinic acid productivity while producing little or no organic acids, as compared to the prior strains producing succinic acid. Thus, the inventive mutant microorganism is useful to produce succinic acid for industrial use.

公开(公告)号：US09428774B2

公开(公告)日：2016-08-30

申请号：US14874385

申请日：2015-10-03

Applicant: Korea Advanced Institute of Science and Technology

Inventor： Sang Yup Lee ,  Sung Won Lim ,  Hyohak Song

IPC: C12P7/46 ,  C12N1/20 ,  C12N15/90 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12

CPC classification number: C12P7/46 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1217 ,  C12N15/902 ,  C12Y203/01054 ,  Y02P20/52

Abstract: The present invention relates to a mutant microorganism, which is selected from the group consisting of genus Mannheimia, genus Actinobacillus and genus Anaerobiospirillum, producing homo-succinic acid and a method for producing homo-succinic acid using the same, and more particularly to a mutant microorganism producing succinic acid at a high concentration while producing little or no other organic acids in anaerobic conditions, which is obtained by disrupting a gene encoding lactate dehydrogenase (ldhA), a gene encoding phosphotransacetylase (pta), and a gene encoding acetate kinase (ackA), without disrupting a gene encoding pyruvate formate lyase (pfl), as well as a method for producing succinic acid using the same. The inventive mutant microorganism has the property of having a high growth rate and succinic acid productivity while producing little or no organic acids, as compared to the prior strains producing succinic acid. Thus, the inventive mutant microorganism is useful to produce succinic acid for industrial use.