公开(公告)号：US20160084860A1

公开(公告)日：2016-03-24

申请号：US14857661

申请日：2015-09-17

Applicant: LI-COR, INC.

Inventor： NISHA PADHYE ,  Rose Skopp ,  Ying Wang

IPC: G01N33/96 ,  G01N33/58 ,  C07K7/06

CPC classification number: G01N33/96 ,  C07K7/06 ,  C07K14/795 ,  C12Q1/28 ,  G01N33/581 ,  G01N33/582 ,  G01N2333/908 ,  G01N2550/00

Abstract: Multifunctional molecular weight protein ladders and methods of making thereof are disclosed herein that are useful for determining the molecular weight of a test protein and/or the relative mass or amount of the test protein in a protein separation assay, such as gel electrophoresis or western blotting. Also included are compounds of Formula I (e.g., mono acetylated MP-11 NHS ester) that may be used to label purified proteins of the protein ladder. The MP-11 label protein ladder can be detected on a blotting membrane by exposing the microperoxidase to a suitable substrate, such as a chromogenic substrate or a chemiluminescent substrate.

Abstract translation: 本文公开了多功能分子量蛋白质梯子及其制备方法，其可用于测定蛋白质分离测定（例如凝胶电泳或western印迹法）中测试蛋白质的分子量和/或测试蛋白质的相对质量或量 。 还包括可用于标记蛋白质梯度蛋白的式I化合物（例如，单乙酰化MP-11NNS酯）。 通过将微过氧化物酶暴露于合适的底物例如显色底​​物或化学发光底物，可以在印迹膜上检测MP-11标记蛋白梯度。