公开(公告)号：US10365290B2

公开(公告)日：2019-07-30

申请号：US15491905

申请日：2017-04-19

Applicant: LI-COR, Inc.

Inventor： Nisha Padhye ,  Rose Skopp ,  Ying Wang

IPC: C07K7/06 ,  G01N33/58 ,  G01N33/96 ,  C07K14/795 ,  C12Q1/28

Abstract: Multifunctional molecular weight protein ladders and methods of making thereof are disclosed herein that are useful for determining the molecular weight of a test protein and/or the relative mass or amount of the test protein in a protein separation assay, such as gel electrophoresis or western blotting. Also included are compounds of Formula I (e.g., mono acetylated MP-11 NHS ester) that may be used to label purified proteins of the protein ladder. The MP-11 label protein ladder can be detected on a blotting membrane by exposing the microperoxidase to a suitable substrate, such as a chromogenic substrate or a chemiluminescent substrate.

公开(公告)号：US09663554B2

公开(公告)日：2017-05-30

申请号：US14857661

申请日：2015-09-17

Applicant: LI-COR, INC.

Inventor： Nisha Padhye ,  Rose Skopp ,  Ying Wang

IPC: G01N33/58 ,  G01N33/96 ,  C07K7/06 ,  C07K14/795 ,  C12Q1/28

CPC classification number: G01N33/96 ,  C07K7/06 ,  C07K14/795 ,  C12Q1/28 ,  G01N33/581 ,  G01N33/582 ,  G01N2333/908 ,  G01N2550/00

Abstract: Multifunctional molecular weight protein ladders and methods of making thereof are disclosed herein that are useful for determining the molecular weight of a test protein and/or the relative mass or amount of the test protein in a protein separation assay, such as gel electrophoresis or western blotting. Also included are compounds of Formula I (e.g., mono acetylated MP-11 NHS ester) that may be used to label purified proteins of the protein ladder. The MP-11 label protein ladder can be detected on a blotting membrane by exposing the microperoxidase to a suitable substrate, such as a chromogenic substrate or a chemiluminescent substrate.

公开(公告)号：US20170219613A1

公开(公告)日：2017-08-03

申请号：US15491905

申请日：2017-04-19

Applicant: LI-COR, Inc.

Inventor： Nisha Padhye ,  Rose Skopp ,  Ying Wang

IPC: G01N33/96 ,  G01N33/58 ,  C07K7/06

CPC classification number: G01N33/96 ,  C07K7/06 ,  C07K14/795 ,  C12Q1/28 ,  G01N33/581 ,  G01N33/582 ,  G01N2333/908 ,  G01N2550/00

Abstract: Multifunctional molecular weight protein ladders and methods of making thereof are disclosed herein that are useful for determining the molecular weight of a test protein and/or the relative mass or amount of the test protein in a protein separation assay, such as gel electrophoresis or western blotting. Also included are compounds of Formula I (e.g., mono acetylated MP-11 NHS ester) that may be used to label purified proteins of the protein ladder. The MP-11 label protein ladder can be detected on a blotting membrane by exposing the microperoxidase to a suitable substrate, such as a chromogenic substrate or a chemiluminescent substrate.

公开(公告)号：US09632034B2

公开(公告)日：2017-04-25

申请号：US15068373

申请日：2016-03-11

Applicant: LI-COR, Inc.

Inventor： Ying Wang ,  Xinshe Xu ,  Nisha Padhye

IPC: G01N21/78 ,  C12Q1/28 ,  G01N33/58 ,  G01N21/76

CPC classification number: G01N21/78 ,  C12Q1/28 ,  G01N21/76 ,  G01N33/582

Abstract: The present invention provides near-infrared fluorogenic and/or chromogenic formulations, methods, systems and kits. Upon contacting an azine with an oxidant and a peroxidase, the azine is converted to an azine derivative which is both visibly colored and fluorescent. This invention also provides high sensitive methods to detect biological molecules, showing better sensitivity than chemiluminescence methods. Advantageously, the formulation and reaction is free of luminol or luminol derivatives.

公开(公告)号：US20160266045A1

公开(公告)日：2016-09-15

申请号：US15068373

申请日：2016-03-11

Applicant: LI-COR, Inc.

Inventor： Ying Wang ,  Xinshe Xu ,  Nisha Padhye

IPC: G01N21/78 ,  G01N33/58 ,  G01N21/76 ,  C12Q1/28

CPC classification number: G01N21/78 ,  C12Q1/28 ,  G01N21/76 ,  G01N33/582

Abstract: The present invention provides near-infrared fluorogenic and/or chromogenic formulations, methods, systems and kits. Upon contacting an azine with an oxidant and a peroxidase, the azine is converted to an azine derivative which is both visibly colored and fluorescent. This invention also provides high sensitive methods to detect biological molecules, showing better sensitivity than chemiluminescence methods. Advantageously, the formulation and reaction is free of luminol or luminol derivatives.

Abstract translation: 本发明提供近红外荧光和/或显色制剂，方法，系统和试剂盒。 当将吖嗪与氧化剂和过氧化物酶接触时，吖嗪被转化成浑浊的吖嗪衍生物，它们都是明显的和荧光的。 本发明还提供了检测生物分子的高灵敏度方法，显示出比化学发光方法更好的灵敏度。 有利地，制剂和反应没有鲁米诺或鲁米诺衍生物。

公开(公告)号：US20160084860A1

公开(公告)日：2016-03-24

申请号：US14857661

申请日：2015-09-17

Applicant: LI-COR, INC.

Inventor： NISHA PADHYE ,  Rose Skopp ,  Ying Wang

IPC: G01N33/96 ,  G01N33/58 ,  C07K7/06

CPC classification number: G01N33/96 ,  C07K7/06 ,  C07K14/795 ,  C12Q1/28 ,  G01N33/581 ,  G01N33/582 ,  G01N2333/908 ,  G01N2550/00

Abstract: Multifunctional molecular weight protein ladders and methods of making thereof are disclosed herein that are useful for determining the molecular weight of a test protein and/or the relative mass or amount of the test protein in a protein separation assay, such as gel electrophoresis or western blotting. Also included are compounds of Formula I (e.g., mono acetylated MP-11 NHS ester) that may be used to label purified proteins of the protein ladder. The MP-11 label protein ladder can be detected on a blotting membrane by exposing the microperoxidase to a suitable substrate, such as a chromogenic substrate or a chemiluminescent substrate.

Abstract translation: 本文公开了多功能分子量蛋白质梯子及其制备方法，其可用于测定蛋白质分离测定（例如凝胶电泳或western印迹法）中测试蛋白质的分子量和/或测试蛋白质的相对质量或量 。 还包括可用于标记蛋白质梯度蛋白的式I化合物（例如，单乙酰化MP-11NNS酯）。 通过将微过氧化物酶暴露于合适的底物例如显色底​​物或化学发光底物，可以在印迹膜上检测MP-11标记蛋白梯度。