公开(公告)号：US20190024077A1

公开(公告)日：2019-01-24

申请号：US16137448

申请日：2018-09-20

Applicant: LIFE TECHNOLOGIES AS

Inventor： Darren ELLIS ,  Hannah LINDSTROEM

IPC: C12N15/10

Abstract: The disclosure generally relates to compositions and methods for the extraction of nucleic acids from biological samples. In particular embodiments the extraction involves the use of an organic solvent with sufficient density so that centrifugation of the sample is not needed to achieve phase separation.

公开(公告)号：US20180163255A1

公开(公告)日：2018-06-14

申请号：US15891535

申请日：2018-02-08

Applicant: LIFE TECHNOLOGIES AS

Inventor： Darren ELLIS ,  Geir FONNUM ,  Nini KJUS

IPC: C12Q1/6806 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12N15/1013

Abstract: A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support. The polyol may for example be a C2-C10 alkanediol.

公开(公告)号：US20170204398A1

公开(公告)日：2017-07-20

申请号：US15431678

申请日：2017-02-13

Applicant: LIFE TECHNOLOGIES AS

Inventor： Darren ELLIS ,  Geir FONNUM ,  Nini KJUS

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12N15/1013

Abstract: A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support. The polyol may for example be a C2-C10 alkanediol.