公开(公告)号：US20140170710A1

公开(公告)日：2014-06-19

申请号：US14167932

申请日：2014-01-29

Applicant: Life Technologies Corporation

Inventor： Jonathan CHESNUT ,  Miroslav Dudas ,  Adam Harris ,  Louis Leong ,  Knut Madden

IPC: C12N15/66

CPC classification number: C12P19/34 ,  C07H21/04 ,  C12N9/90 ,  C12N15/10 ,  C12N15/111 ,  C12N15/64 ,  C12N15/66 ,  C12N15/86 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2330/30 ,  C12N2740/16043 ,  C12N2800/30 ,  C12N2800/70 ,  C12Y599/01

Abstract: The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention.

Abstract translation: 本发明涉及生物技术和分子生物学领域。 更具体地说，本发明涉及克隆或亚克隆一个或多个包含一个或多个II型限制酶识别位点的核酸分子。 本发明还使用重组克隆方法（例如使用重组位点和重组蛋白的那些）来体现克隆这种核酸分子。 本发明还涉及由使用本发明的方法生产的宿主细胞表达的核酸分子（包括RNA和iRNA）以及蛋白质。

公开(公告)号：US20130157263A1

公开(公告)日：2013-06-20

申请号：US13666923

申请日：2012-11-01

Applicant: Life Technologies Corporation

Inventor： Jonathan CHESNUT ,  Knut Madden ,  Miroslav Dudas ,  Louis Leong ,  Adam Harris

IPC: C07H21/04

CPC classification number: C12P19/34 ,  C07H21/04 ,  C12N9/90 ,  C12N15/10 ,  C12N15/111 ,  C12N15/64 ,  C12N15/66 ,  C12N15/86 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2330/30 ,  C12N2740/16043 ,  C12N2800/30 ,  C12N2800/70 ,  C12Y599/01

Abstract: The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention.

公开(公告)号：US20190203196A1

公开(公告)日：2019-07-04

申请号：US16206193

申请日：2018-11-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Knut MADDEN ,  Adam Harris ,  Karl Hecker ,  Byung-In Lee

IPC: C12N15/10 ,  C12Q1/6806 ,  C07H21/02 ,  C12N15/11

CPC classification number: C12N15/101 ,  B01D15/3804 ,  C07H21/02 ,  C12N15/111 ,  C12N2330/31 ,  C12Q1/6806 ,  C12Q2525/204

Abstract: The invention provides methods of preparing nucleic acids, such as RNA molecules, of a defined size or range of sizes. The invention provides compositions, methods and kits for use in the production and preparation of small RNA molecules (including without limitation micro-RNA, siRNA, d-siRNA and e-siRNA) and other nucleic acids of various sizes.

公开(公告)号：US20140142290A1

公开(公告)日：2014-05-22

申请号：US14028247

申请日：2013-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Knut MADDEN ,  Adam Harris ,  Karl Hecker ,  Byung-In Lee

IPC: C12N15/10

CPC classification number: C12N15/101 ,  B01D15/3804 ,  C07H21/02 ,  C12N15/111 ,  C12N2330/31 ,  C12Q1/6806 ,  C12Q2525/204

Abstract: The invention provides methods of preparing nucleic acids, such as RNA molecules, of a defined size or range of sizes. The invention provides compositions, methods and kits for use in the production and preparation of small RNA molecules (including without limitation micro-RNA, siRNA, d-siRNA and e-siRNA) and other nucleic acids of various sizes.

Abstract translation: 本发明提供了制备具有规定尺寸或尺寸范围的核酸如RNA分子的方法。 本发明提供了用于生产和制备小RNA分子（包括但不限于微RNA，siRNA，d-siRNA和e-siRNA）和各种尺寸的其它核酸的组合物，方法和试剂盒。