公开(公告)号：US20190106730A1

公开(公告)日：2019-04-11

申请号：US16212011

申请日：2018-12-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan WANG ,  David DUPONT

IPC: C12Q1/686 ,  C12Q1/6844 ,  C12N9/12

Abstract: This disclosure relates to the use of one or more proteins (e.g., globular proteins) having a low isoelectric point and/or a limited number (e.g., zero) of modifying groups in nucleic acid polymerization and/or amplification reactions such as polymerase chain reaction (PCR).

公开(公告)号：US20170292144A1

公开(公告)日：2017-10-12

申请号：US15481021

申请日：2017-04-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ferrier LE ,  Kalpith RAMAMOORTHI ,  Joyce WILDE ,  Nicole FANTIN ,  Jordan LANG ,  David DUPONT ,  Junko STEVENS

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  B01D19/0404 ,  B01D19/0409 ,  B01D19/0413 ,  B01D19/0418 ,  B01D19/0422 ,  C12Q1/6846 ,  C12Q2525/125

Abstract: Compositions, methods, and kits for synthesizing, detecting, and/or quantifying nucleic acids are provided herein. Embodiments comprise a nucleic acid amplification composition comprising a thermostable DNA polymerase and agents which improve nucleic acid synthesis, amplification, detection, and/or quantification of nucleic acid targets in a crude extract or crude lysate sample.

公开(公告)号：US20170114391A1

公开(公告)日：2017-04-27

申请号：US15397938

申请日：2017-01-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan WANG ,  David DUPONT

IPC: C12Q1/68 ,  C12N9/12

CPC classification number: C12Q1/686 ,  C12N9/1252 ,  C12Q1/6846 ,  C12Y207/07007 ,  C12Q2527/125

Abstract: This disclosure relates to the use of one or more proteins (e.g., globular proteins) having a low isoelectric point and/or a limited number (e.g., zero) of modifying groups in nucleic acid polymerization and/or amplification reactions such as polymerase chain reaction (PCR).

公开(公告)号：US20210277448A1

公开(公告)日：2021-09-09

申请号：US17301040

申请日：2021-03-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ferrier LE ,  Kalpith RAMAMOORTHI ,  Joyce WILDE ,  Nicole FANTIN ,  Jordan LANG ,  David DUPONT ,  Junko STEVENS

IPC: C12Q1/686 ,  C12Q1/6844

Abstract: Compositions, methods, and kits for synthesizing, detecting, and/or quantifying nucleic acids are provided herein. Embodiments comprise a nucleic acid amplification composition comprising a thermostable DNA polymerase and agents which improve nucleic acid synthesis, amplification, detection, and/or quantification of nucleic acid targets in a crude extract or crude lysate sample.