公开(公告)号：US20170114391A1

公开(公告)日：2017-04-27

申请号：US15397938

申请日：2017-01-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan WANG ,  David DUPONT

IPC: C12Q1/68 ,  C12N9/12

CPC classification number: C12Q1/686 ,  C12N9/1252 ,  C12Q1/6846 ,  C12Y207/07007 ,  C12Q2527/125

Abstract: This disclosure relates to the use of one or more proteins (e.g., globular proteins) having a low isoelectric point and/or a limited number (e.g., zero) of modifying groups in nucleic acid polymerization and/or amplification reactions such as polymerase chain reaction (PCR).

公开(公告)号：US20210269871A1

公开(公告)日：2021-09-02

申请号：US17193232

申请日：2021-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Swati GOYAL ,  Achim KARGER ,  Peter MA ,  S. Jeffrey ROSNER ,  Ian WALTON ,  Jonathan WANG ,  Michael WENZ

IPC: C12Q1/6869 ,  B01L3/00 ,  C12Q1/6806

Abstract: According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.

公开(公告)号：US20190106730A1

公开(公告)日：2019-04-11

申请号：US16212011

申请日：2018-12-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan WANG ,  David DUPONT

IPC: C12Q1/686 ,  C12Q1/6844 ,  C12N9/12

Abstract: This disclosure relates to the use of one or more proteins (e.g., globular proteins) having a low isoelectric point and/or a limited number (e.g., zero) of modifying groups in nucleic acid polymerization and/or amplification reactions such as polymerase chain reaction (PCR).

公开(公告)号：US20170073746A1

公开(公告)日：2017-03-16

申请号：US15273824

申请日：2016-09-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul ANGRISH ,  Zhiwei YANG ,  Jonathan WANG

IPC: C12Q1/68

CPC classification number: C12Q1/6848 ,  C07C229/12 ,  C07C229/24 ,  C07D207/16 ,  C07D211/60 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6876 ,  C12Q2600/158 ,  C12Q2527/125

Abstract: This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (PCR). Methods for preparing the modified detergents are also described.

Abstract translation: 本公开涉及用于各种方法的新型洗涤剂，包括例如核酸扩增反应如聚合酶链式反应（PCR）。 还描述了制备改性洗涤剂的方法。

公开(公告)号：US20230212662A1

公开(公告)日：2023-07-06

申请号：US18087735

申请日：2022-12-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul ANGRISH ,  Zhiwei YANG ,  Jonathan WANG

IPC: C12Q1/6848 ,  C07C229/12 ,  C07C229/24 ,  C07D207/16 ,  C12Q1/6844 ,  C07D211/60 ,  C12Q1/6806 ,  C12Q1/6876

CPC classification number: C12Q1/6848 ,  C07C229/12 ,  C07C229/24 ,  C07D207/16 ,  C12Q1/6844 ,  C07D211/60 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2600/158

Abstract: This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (PCR). Methods for preparing the modified detergents are also described.

公开(公告)号：US20140308666A1

公开(公告)日：2014-10-16

申请号：US14250154

申请日：2014-04-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul ANGRISH ,  Zhiwei YANG ,  Jonathan WANG

IPC: C12Q1/68

CPC classification number: C12Q1/6848 ,  C07C229/12 ,  C07C229/24 ,  C07D207/16 ,  C07D211/60 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6876 ,  C12Q2600/158 ,  C12Q2527/125

Abstract: This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (PCR). Methods for preparing the modified detergents are also described.

Abstract translation: 本公开涉及用于各种方法的新型洗涤剂，包括例如核酸扩增反应如聚合酶链式反应（PCR）。 还描述了制备改性洗涤剂的方法。

公开(公告)号：US20200340048A1

公开(公告)日：2020-10-29

申请号：US16870398

申请日：2020-05-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul ANGRISH ,  Zhiwei YANG ,  Jonathan WANG

IPC: C12Q1/6848 ,  C07C229/12 ,  C07C229/24 ,  C07D207/16 ,  C12Q1/6844 ,  C07D211/60 ,  C12Q1/6806 ,  C12Q1/6876

Abstract: This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (PCR). Methods for preparing the modified detergents are also described.

公开(公告)号：US20190119739A1

公开(公告)日：2019-04-25

申请号：US16225261

申请日：2018-12-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul ANGRISH ,  Zhiwei YANG ,  Jonathan WANG

IPC: C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6876 ,  C12Q1/6806 ,  C07C229/12 ,  C07D211/60 ,  C07D207/16 ,  C07C229/24

CPC classification number: C12Q1/6848 ,  C07C229/12 ,  C07C229/24 ,  C07D207/16 ,  C07D211/60 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6876 ,  C12Q2600/158 ,  C12Q2527/125

Abstract: This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (PCR). Methods for preparing the modified detergents are also described.