公开(公告)号：US20180010177A1

公开(公告)日：2018-01-11

申请号：US15695244

申请日：2017-09-05

Applicant: Life Technologies Corporation

Inventor： Stephen HENDRICKS ,  David KING ,  Lei XI ,  Marian PERIS

IPC: C12Q1/68 ,  C12Q1/46 ,  C12P19/34

CPC classification number: C12Q1/46 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

公开(公告)号：US20190024159A1

公开(公告)日：2019-01-24

申请号：US16154529

申请日：2018-10-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen HENDRICKS ,  David KING ,  Lei XI ,  Marian PERIS

IPC: C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6874 ,  C12Q1/6844 ,  C12Q1/46 ,  C12P19/34

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.