公开(公告)号：US20240002930A1

公开(公告)日：2024-01-04

申请号：US18303828

申请日：2023-04-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen HENDRICKS ,  David J. KING

IPC: C12Q1/6874 ,  A61J1/06 ,  A61J1/14 ,  B65B3/00 ,  B65B31/04 ,  B65D8/00 ,  C12N9/00 ,  C12Q1/6876

CPC classification number: C12Q1/6874 ,  A61J1/062 ,  A61J1/14 ,  B65B3/003 ,  B65B3/006 ,  B65B31/046 ,  B65D15/02 ,  C12N9/93 ,  C12Q1/6876 ,  A61J2200/76 ,  A61J2205/30 ,  A61M5/008

Abstract: Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.

公开(公告)号：US20160304844A1

公开(公告)日：2016-10-20

申请号：US15200670

申请日：2016-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Marian PERIS ,  Michael PHELAN ,  Barnett ROSENBLUM ,  Stephen HENDRICKS

IPC: C12N9/12 ,  C12Q1/68

CPC classification number: C12N9/1252 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Y207/07007 ,  G01N2500/00 ,  C12Q2565/632 ,  C12Q2563/137 ,  C12Q2563/107 ,  C12Q2565/30 ,  C12Q2537/157

Abstract: Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.

Abstract translation: 本文提供了衍生自野生型RB69 DNA聚合酶或与野生型RB69 DNA聚合酶相关的突变型DNA依赖性聚合酶。 这些突变型聚合酶能够选择性地结合标记的核苷酸。 这些突变型聚合酶还能够掺入多种天然存在和修饰的核苷酸，包括例如终止子核苷酸。

公开(公告)号：US20170175092A1

公开(公告)日：2017-06-22

申请号：US15422224

申请日：2017-02-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Marian PERIS ,  Michael PHELAN ,  Barnett ROSENBLUM ,  Stephen HENDRICKS

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Y207/07007 ,  G01N2500/00 ,  C12Q2565/632 ,  C12Q2563/137 ,  C12Q2563/107 ,  C12Q2565/30 ,  C12Q2537/157

Abstract: Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.

公开(公告)号：US20160230156A1

公开(公告)日：2016-08-11

申请号：US14776856

申请日：2014-03-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen HENDRICKS

IPC: C12N9/22 ,  C12N9/16

CPC classification number: C12N9/22 ,  C07K2319/00 ,  C12N9/16 ,  C12Y301/03001 ,  C12Y301/11001

Abstract: Disclosed herein is a fusion protein possessing both nuclease and phosphatase activities. The described fusion protein simplifies the processing of amplified DNA to degrade residual primers and nucleotide triphosphates and thereby facilitates subsequent DNA analysis.

Abstract translation: 本文公开了具有核酸酶和磷酸酶活性的融合蛋白。 所述的融合蛋白简化了扩增DNA的加工以降解残留引物和核苷酸三磷酸，从而有助于随后的DNA分析。

公开(公告)号：US20140234940A1

公开(公告)日：2014-08-21

申请号：US14191997

申请日：2014-02-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Marian PERIS ,  Michael PHELAN ,  Barnett ROSENBLUM ,  Stephen HENDRICKS

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Y207/07007 ,  G01N2500/00 ,  C12Q2565/632 ,  C12Q2563/137 ,  C12Q2563/107 ,  C12Q2565/30 ,  C12Q2537/157

Abstract: Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.

Abstract translation: 本文提供了衍生自野生型RB69 DNA聚合酶或与野生型RB69 DNA聚合酶相关的突变型DNA依赖性聚合酶。 这些突变型聚合酶能够选择性地结合标记的核苷酸。 这些突变型聚合酶还能够掺入多种天然存在和修饰的核苷酸，包括例如终止子核苷酸。

公开(公告)号：US20180010177A1

公开(公告)日：2018-01-11

申请号：US15695244

申请日：2017-09-05

Applicant: Life Technologies Corporation

Inventor： Stephen HENDRICKS ,  David KING ,  Lei XI ,  Marian PERIS

IPC: C12Q1/68 ,  C12Q1/46 ,  C12P19/34

CPC classification number: C12Q1/46 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

公开(公告)号：US20170081706A1

公开(公告)日：2017-03-23

申请号：US15282597

申请日：2016-09-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Lei XI ,  Paul KENNEY ,  Zhaochun MA ,  Dennis PROSEN ,  Stephen HENDRICKS ,  Roland NAGEL

IPC: C12Q1/68 ,  C12N9/12 ,  C07H21/02

CPC classification number: C12Q1/686 ,  C07H21/00 ,  C07H21/02 ,  C12N9/1252 ,  C12N15/10 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or μ-monothiopyrophosphate), and related compounds.

公开(公告)号：US20210324460A1

公开(公告)日：2021-10-21

申请号：US17305239

申请日：2021-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shiaw-Min CHEN ,  Elana SWARTZMAN ,  David RUFF ,  Mark SHANNON ,  Julia LU ,  Stephen HENDRICKS

IPC: C12Q1/6855 ,  C12Q1/6804

Abstract: This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.

公开(公告)号：US20200048708A1

公开(公告)日：2020-02-13

申请号：US16666188

申请日：2019-10-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen HENDRICKS ,  David J. KING

IPC: C12Q1/6874 ,  A61J1/14 ,  B65D8/00 ,  B65B3/00 ,  B65B31/04 ,  A61J1/06 ,  C12N9/00 ,  C12Q1/6876

Abstract: Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.

公开(公告)号：US20200048695A1

公开(公告)日：2020-02-13

申请号：US16656839

申请日：2019-10-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shiaw-Min CHEN ,  Elana SWARTZMAN ,  David RUFF ,  Mark SHANNON ,  Julia LU ,  Stephen HENDRICKS

IPC: C12Q1/6855 ,  C12Q1/6804

Abstract: This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.