公开(公告)号：US11072824B2

公开(公告)日：2021-07-27

申请号：US16656839

申请日：2019-10-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shiaw-Min Chen ,  Elana Swartzman ,  David Ruff ,  Mark Shannon ,  Julia Lu ,  Stephen Hendricks

IPC: C12Q1/68 ,  C12Q1/6855 ,  C12Q1/6804

Abstract: This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.

公开(公告)号：US10472671B2

公开(公告)日：2019-11-12

申请号：US14955386

申请日：2015-12-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shiaw-Min Chen ,  Elana Swartzman ,  David Ruff ,  Mark Shannon ,  Julia Lu ,  Stephen Hendricks

IPC: C12Q1/68 ,  C12Q1/6855 ,  C12Q1/6804

Abstract: This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.

公开(公告)号：US20160153035A1

公开(公告)日：2016-06-02

申请号：US14955386

申请日：2015-12-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： SHIAW-MIN CHEN ,  Elana Swartzman ,  David Ruff ,  Mark Shannon ,  Julia Lu ,  Stephen Hendricks

IPC: C12Q1/68

Abstract: This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.

Abstract translation: 本申请涉及用于连接与靶核酸互补的寡核苷酸的方法，并扩增连接的寡核苷酸，其中连接和扩增发生在相同的反应混合物中。