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    METHODS, COMPOSITIONS, AND KITS FOR DETECTING PROTEIN AGGREGATES
    2.
    发明申请
    METHODS, COMPOSITIONS, AND KITS FOR DETECTING PROTEIN AGGREGATES 有权
    用于检测蛋白质聚集体的方法，组合物和试剂盒

    公开(公告)号：US20140134624A1

    公开(公告)日：2014-05-15

    申请号：US13975056

    申请日：2013-08-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： David RUFF ,  Mark Shannon ,  Kenneth Livak ,  Karl Guegler ,  Kevin Hennessy

    IPC: C12Q1/68

    CPC classification number: C12Q1/6876 ,  C07K14/47 ,  C07K14/4711 ,  C12Q1/6816 ,  C12Q2533/107

    Abstract: The present teachings provide methods, compositions, and kits for detecting the presence of protein aggregates. In some embodiments, the protein aggregate is treated with a labeled precursor, and the labeled precursor is incorporated into the protein aggregate to form a labeled protein aggregate. The labeled protein aggregate is then measured, thus detecting the presence of the protein aggregate. In some embodiments, the labeled protein aggregate is detected by interaction of labeled precursors, for example by a proximity ligation assay.

    Abstract translation: 本教导提供了用于检测蛋白质聚集体的存在的方法，组合物和试剂盒。 在一些实施方案中，用标记的前体处理蛋白质聚集体，并将标记的前体掺入蛋白质聚集体中以形成标记的蛋白质聚集体。 然后测量标记的蛋白质聚集体，从而检测蛋白质聚集体的存在。 在一些实施方案中，标记的蛋白质聚集体通过标记的前体的相互作用来检测，例如通过邻近连接测定。

    Biological sample preparation for testing
    3.
    发明授权
    Biological sample preparation for testing 有权

    公开(公告)号：US10371604B2

    公开(公告)日：2019-08-06

    申请号：US15233801

    申请日：2016-08-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mousumi Rath ,  Mark Shannon ,  Jason La

    IPC: G01N1/04 ,  C12Q1/6806 ,  G01N1/28 ,  G01N1/34 ,  G01N1/02 ,  B01L3/00

    Abstract: In one embodiment, a method for processing a sample includes selecting a selected sample from a biological specimen, the selected sample being in contact with a first surface of a first substrate. The method also includes transferring the selected sample directly from the first surface to a container comprising an internal volume. The method also includes forming or providing a sample solution within the internal volume of the container by contacting the selected sample with a lysis mixture using a protocol. The method further includes performing an assay, experiment, or test on the sample solution while the sample solution disposed is within the internal volume of the container.In another embodiment, a method for processing a sample includes providing a selected sample comprising one or more cells. The method also includes transferring the selected sample into an internal volume of a container. The method also includes contacting the selected sample with a lysis mixture using a protocol to provide a sample solution, wherein the protocol comprises heating the sample solution to a first temperature that is greater than 37 degrees Celsius and less than or equal to 75 degrees Celsius.

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