公开(公告)号：US11001815B2

公开(公告)日：2021-05-11

申请号：US16442341

申请日：2019-06-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chieh-Yuan Li ,  David Ruff ,  Shiaw-Min Chen ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874

Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent.

公开(公告)号：US10858695B2

公开(公告)日：2020-12-08

申请号：US16168810

申请日：2018-10-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chieh-Yuan Li ,  David Ruff ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Shiaw-Min Chen ,  Jonathan M. Rothberg ,  Bin Li ,  Kai Qin Lao

IPC: C12Q1/68 ,  C12Q1/6844 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6853

Abstract: In some embodiments, provided are methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

公开(公告)号：US09371557B2

公开(公告)日：2016-06-21

申请号：US13923232

申请日：2013-06-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chieh-Yuan Li ,  David Ruff ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Shiaw-Min Chen ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

Abstract translation: 在一些实施方案中，本教导提供核酸扩增方法，包括形成反应混合物，并使反应混合物经受适合于核酸扩增的条件。 在一些实施方案中，用于核酸扩增的方法包括将待扩增的核酸进行部分变性的条件。 在一些实施方案中，用于核酸扩增的方法包括扩增而不完全变性扩增的核酸。 在一些实施方案中，用于核酸扩增的方法使用催化同源重组的酶和聚合酶。 在一些实施方案中，用于核酸扩增的方法可以在单个反应容器中进行。 在一些实施方案中，用于核酸扩增的方法可以在反应混合物的单个连续液相中进行，而不需要分隔反应混合物或固定反应组分。 在一些实施方案中，用于核酸扩增的方法包括在等温扩增条件下任选地在聚合物存在下在表面上扩增至少一种多核苷酸。 聚合物可以包括筛分剂和/或扩散还原剂。

公开(公告)号：US09309558B2

公开(公告)日：2016-04-12

申请号：US14085727

申请日：2013-11-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chieh-Yuan Li ,  David Ruff ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Shiaw-Min Chen ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

公开(公告)号：US09309557B2

公开(公告)日：2016-04-12

申请号：US13842296

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chieh-Yuan Li ,  David Ruff ,  Shiaw-Min Chen ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

公开(公告)号：US10329544B2

公开(公告)日：2019-06-25

申请号：US15091717

申请日：2016-04-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chieh-Yuan Li ,  David Ruff ,  Shiaw-Min Chen ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao ,  Wolfgang Hinz

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874

Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

公开(公告)号：US10113195B2

公开(公告)日：2018-10-30

申请号：US14789922

申请日：2015-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chieh-Yuan Li ,  David Ruff ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Shiaw-Min Chen ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao

IPC: C12Q1/68 ,  C12Q1/6844 ,  C12Q1/6874

Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

公开(公告)号：US20160153035A1

公开(公告)日：2016-06-02

申请号：US14955386

申请日：2015-12-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： SHIAW-MIN CHEN ,  Elana Swartzman ,  David Ruff ,  Mark Shannon ,  Julia Lu ,  Stephen Hendricks

IPC: C12Q1/68

Abstract: This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.

Abstract translation: 本申请涉及用于连接与靶核酸互补的寡核苷酸的方法，并扩增连接的寡核苷酸，其中连接和扩增发生在相同的反应混合物中。

公开(公告)号：US11725195B2

公开(公告)日：2023-08-15

申请号：US17302192

申请日：2021-04-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chieh-Yuan Li ,  David Ruff ,  Shiaw-Min Chen ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874

CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Y207/07007 ,  C12Q1/6846 ,  C12Q2531/119 ,  C12Q2565/537 ,  C12Q1/6855 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent.

公开(公告)号：US11072824B2

公开(公告)日：2021-07-27

申请号：US16656839

申请日：2019-10-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shiaw-Min Chen ,  Elana Swartzman ,  David Ruff ,  Mark Shannon ,  Julia Lu ,  Stephen Hendricks

IPC: C12Q1/68 ,  C12Q1/6855 ,  C12Q1/6804

Abstract: This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.