公开(公告)号：US20210053058A1

公开(公告)日：2021-02-25

申请号：US16994289

申请日：2020-08-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jon HOSHIZAKI ,  Patrick LEUNG ,  David MARRAN ,  Mark REED

IPC: B01L3/00

Abstract: An apparatus includes a fluidic coupler including an opening. A first port is in fluid communication with the opening and is to interface with an inlet of a flow cell of a sensor device. A second port is to interface with an outlet of the flow cell of the sensor device. A third port is in fluidic communication with the second port. The apparatus further includes a mechanical assembly moveable between a first position and a second position. The fluidic coupler is secured to the flow cell of the sensor device in the first position. The fluidic coupler is disengaged from the flow cell of the sensor device in the second position.

公开(公告)号：US20210054450A1

公开(公告)日：2021-02-25

申请号：US16994318

申请日：2020-08-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark REED ,  Chiu Tai Andrew WONG ,  David MARRAN

IPC: C12Q1/6874

Abstract: A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a run time in a range of 5 hours to 14 hours to determine variant calls for 4 extracted polynucleotide samples using a targeted assay with one DNA pool per sample and an average amplicon size in a range of 100 to 120 bases.

公开(公告)号：US20220205033A1

公开(公告)日：2022-06-30

申请号：US17553295

申请日：2021-12-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark REED ,  Joseph PODHASKY ,  Shanti SHANKAR

IPC: C12Q1/6869 ,  C12Q1/6806 ,  G16B50/20

Abstract: A method for determining a sequence of nucleic acids includes purifying the nucleic acids from a sample with a purification instrument in accordance with a purification plan of a run plan. The purified nucleic acids are disposed in a transfer plate. Disposition of the purified nucleic acids is stored in a transfer file. The method further includes transferring the transfer plate to a sequencing instrument; transferring the transfer file to the sequencing instrument; and sequencing the nucleic acids with the sequencing instrument in accordance with a sequencing plan of the run plan and based on the transfer file.

公开(公告)号：US20200376492A1

公开(公告)日：2020-12-03

申请号：US16885904

申请日：2020-05-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark REED ,  Rui ZHENG

IPC: B01L3/00

Abstract: A container includes a lower portion and a lid to connect with the lower portion. The lid includes a fluidic interface, a swing bar, and a counter structure disposed on an opposite side of the fluidic interface relative to the swing bar. The fluidic interface includes a first liquid port, a second liquid port, a gas port, and a seal ring disposed around the first liquid port, the second liquid port, and the gas port, the first liquid port disposed along a center axis of the container.

公开(公告)号：US20210055255A1

公开(公告)日：2021-02-25

申请号：US16994202

申请日：2020-08-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Creighton BUIE ,  Mark REED ,  Albert CARRILLO

IPC: G01N27/414

Abstract: A sensor device includes a substrate having a die attached to the substrate. The die includes an array of sensors and an array of wells cooperatively disposed over the array of sensors. The array of wells is exposed by the substrate. The sensor device further includes a flow cell secured to the substrate and defining a flow space disposed over the die and accessible to the array of wells. The flow cell defines a plurality of separate volumes. Each separate volume of the plurality of separate volumes has an inlet and an outlet. The plurality of separate volumes are separated by dividers. The dividers cover a set of wells of the array of wells.

公开(公告)号：US20230226548A1

公开(公告)日：2023-07-20

申请号：US18107887

申请日：2023-02-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jon HOSHIZAKI ,  Patrick LEUNG ,  David MARRAN ,  Mark REED

IPC: B01L3/00

CPC classification number: B01L3/502738 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2300/0816

Abstract: A fluidic coupler to engage a plurality of flow cells of a sensor device includes a body and a plurality of fluidics interfaces formed in the body. Each fluidic interface of the plurality of fluidics interfaces includes an opening, a first port in fluid communication with the opening, a second port, and a third port in fluidic communication with the second port.

公开(公告)号：US20220170093A1

公开(公告)日：2022-06-02

申请号：US17527997

申请日：2021-11-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Joseph PODHASKY ,  Mark REED ,  David MARRAN ,  Chiu Tai Andrew WONG ,  Angie CHENG ,  Ru CAO

IPC: C12Q1/6874 ,  B01J19/00

Abstract: A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a total instrument run time in a range of 16 hours to 22 hours to determine variant calls when sequencing 6 cfTNA extracted polynucleotide samples using a targeted assay with one nucleic acid pool per sample.

公开(公告)号：US20200055046A1

公开(公告)日：2020-02-20

申请号：US16543421

申请日：2019-08-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark REED ,  Zheng (Derek) Xing ,  David Marran ,  Earl Zergiebel

IPC: B01L3/00 ,  C12Q1/6874

Abstract: In an example, a method for preparing a nucleotide solution includes flowing an aqueous solution from an initial solution storage of a sequencing instrument continuously through a container fluidically coupled to the sequencing instrument, the container comprising a nucleotide concentrate; and collecting the aqueous solution with nucleotide in a storage container.