公开(公告)号：US20230194464A1

公开(公告)日：2023-06-22

申请号：US17937797

申请日：2022-10-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Marcin SIKORA ,  Melville DAVEY ,  Christian KOLLER ,  Simon CAWLEY ,  Alan WILLIAMS ,  David KULP

IPC: G01N27/414 ,  G16B30/00 ,  G16B30/10 ,  C12Q1/6869

CPC classification number: G01N27/4145 ,  G16B30/00 ,  G16B30/10 ,  C12Q1/6869 ,  G01N27/27

Abstract: A method for nucleic acid sequencing includes receiving a plurality of observed or measured signals indicative of a parameter observed or measured for a plurality of defined spaces; determining, for at least some of the defined spaces, whether the defined space comprises one or more sample nucleic acids; processing, for at least some of the defined spaces, the observed or measured signal to improve a quality of the observed or measured signal; generating, for at least some of the defined spaces, a set of candidate sequences of bases for the defined space using one or more metrics adapted to associate a score or penalty to the candidate sequences of bases; and selecting the candidate sequence leading to a highest score or a lowest penalty as corresponding to the correct sequence for the one or more sample nucleic acids in the defined space.

公开(公告)号：US20210116414A1

公开(公告)日：2021-04-22

申请号：US17063621

申请日：2020-10-05

Applicant: Life Technologies Corporation

Inventor： Todd REARICK ,  Melville DAVEY ,  Mark BEAUCHEMIN

IPC: G01N27/416 ,  G16B30/00 ,  G01N27/414

Abstract: A method, computer program product, and system are provided to calibrate a sensor array with a plurality of sensors. The method can include sweeping a voltage of a reference electrode from a first voltage to a second voltage, where the reference electrode is in fluid communication with the sensor array. The output voltage of each of the plurality of sensors can be monitored at one or more voltages within the first and second voltages. An overall average gain of the plurality of sensors can be calculated at each of the one or more voltages. Further, an acquisition window for the sensor array can be determined. The acquisition window can include a maximum distribution of sensors that provides a maximal overall average gain at a particular reference electrode voltage.

公开(公告)号：US20190277799A1

公开(公告)日：2019-09-12

申请号：US16271979

申请日：2019-02-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Melville DAVEY ,  Simon CAWLEY ,  Alan WILLIAMS

IPC: G01N27/414 ,  G16B30/00

Abstract: A method for nucleic acid sequencing includes receiving a plurality of signals indicative of a parameter measured for a plurality of defined spaces, at least some of the defined spaces including one or more sample nucleic acids, the signals being responsive to a plurality of nucleotide flows introducing nucleotides to the defined spaces; determining, for at least some of the defined spaces, whether the defined space includes a sample nucleic acid; processing, for at least some of the defined spaces determined to include a sample nucleic acid, the received signals to improve a quality of the received signals; and predicting a plurality of nucleotide sequences corresponding to respective sample nucleic acids for the defined spaces based on the processed signals and the nucleotide flows.

公开(公告)号：US20180313786A1

公开(公告)日：2018-11-01

申请号：US15920387

申请日：2018-03-13

Applicant: Life Technologies Corporation

Inventor： Todd REARICK ,  Melville DAVEY ,  Mark BEAUCHEMIN

IPC: G01N27/416 ,  G01N27/414 ,  G06F19/22

CPC classification number: G01N27/4165 ,  G01N27/4145 ,  G16B30/00

Abstract: A method, computer program product, and system are provided to calibrate a sensor array with a plurality of sensors. The method can include sweeping a voltage of a reference electrode from a first voltage to a second voltage, where the reference electrode is in fluid communication with the sensor array. The output voltage of each of the plurality of sensors can be monitored at one or more voltages within the first and second voltages. An overall average gain of the plurality of sensors can be calculated at each of the one or more voltages. Further, an acquisition window for the sensor array can be determined. The acquisition window can include a maximum distribution of sensors that provides a maximal overall average gain at a particular reference electrode voltage.

公开(公告)号：US20240361272A1

公开(公告)日：2024-10-31

申请号：US18656792

申请日：2024-05-07

Applicant: Life Technologies Corporation

Inventor： Melville DAVEY ,  Simon CAWLEY ,  Alan WILLIAMS

IPC: G01N27/414 ,  G01N27/27 ,  G16B30/00 ,  G16B30/10 ,  G16B30/20

CPC classification number: G01N27/4145 ,  G16B30/00 ,  G16B30/10 ,  G16B30/20 ,  G01N27/27

Abstract: A method for nucleic acid sequencing includes receiving a plurality of signals indicative of a parameter measured for a plurality of defined spaces, at least some of the defined spaces including one or more sample nucleic acids, the signals being responsive to a plurality of nucleotide flows introducing nucleotides to the defined spaces; determining, for at least some of the defined spaces, whether the defined space includes a sample nucleic acid; processing, for at least some of the defined spaces determined to include a sample nucleic acid, the received signals to improve a quality of the received signals; and predicting a plurality of nucleotide sequences corresponding to respective sample nucleic acids for the defined spaces based on the processed signals and the nucleotide flows.

公开(公告)号：US20140324376A1

公开(公告)日：2014-10-30

申请号：US14330756

申请日：2014-07-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd REARICK ,  Melville DAVEY ,  Mark BEAUCHEMIN

IPC: G01N27/416

CPC classification number: G01N27/4165 ,  G01N27/4145 ,  G06F19/22

Abstract: A method, computer program product, and system are provided to calibrate a sensor array with a plurality of sensors. The method can include sweeping a voltage of a reference electrode from a first voltage to a second voltage, where the reference electrode is in fluid communication with the sensor array. The output voltage of each of the plurality of sensors can be monitored at one or more voltages within the first and second voltages. An overall average gain of the plurality of sensors can be calculated at each of the one or more voltages. Further, an acquisition window for the sensor may can be determined. The acquisition window can include a maximum distribution of sensors that provides a maximal overall average gain at a particular reference electrode voltage.

Abstract translation: 提供了一种方法，计算机程序产品和系统来校准具有多个传感器的传感器阵列。 该方法可以包括将参考电极的电压从第一电压扫描到第二电压，其中参考电极与传感器阵列流体连通。 可以在第一和第二电压内的一个或多个电压监视多个传感器中的每一个的输出电压。 可以在一个或多个电压中的每一个处计算多个传感器的总体平均增益。 此外，可以确定传感器的采集窗口。 采集窗口可以包括在特定参考电极电压下提供最大总平均增益的传感器的最大分布。

公开(公告)号：US20140051584A1

公开(公告)日：2014-02-20

申请号：US13967665

申请日：2013-08-15

Applicant: Life Technologies Corporation

Inventor： Melville DAVEY ,  Michael MEYER ,  Marcin SIKORA ,  Simon CAWLEY ,  Kirk PASTORIAN

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  G06F19/10 ,  C12Q2537/165

Abstract: A method of estimating a parameter related to sequencing of a sample nucleic acid template includes: receiving signal data relating to nucleotide incorporation events resulting from a series of flows of nucleotides onto an array of wells including (i) a first well containing the sample nucleic acid template and (ii) a plurality of other sample-containing wells; determining sequence information for the sample nucleic acid template using signal data from the first well; and constructing a phase-state model for a set of nucleotide flows that contributed at least in part to the sequence information, wherein the model includes a signal correction parameter that is determined using signal data from the plurality of other sample-containing wells.

Abstract translation: 估计与样品核酸模板的测序有关的参数的方法包括：接收与一系列核苷酸产生的核苷酸掺入事件相关的信号数据，其包含（i）包含样品核酸的第一阱 模板和（ii）多个其他含样品的孔; 使用来自第一孔的信号数据确定样品核酸模板的序列信息; 以及构建至少部分地对所述序列信息贡献的一组核苷酸流的相态模型，其中所述模型包括使用来自所述多个其它含样品孔的信号数据确定的信号校正参数。