公开(公告)号：US20140296093A1

公开(公告)日：2014-10-02

申请号：US14194486

申请日：2014-02-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael BRASCH ,  Devon Byrd ,  David Cheo ,  James Hartley ,  Gary Temple

IPC: C12N15/10 ,  C12N15/66

CPC classification number: C12N15/1086 ,  C07K14/47 ,  C12N9/00 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1093 ,  C12N15/64 ,  C12N15/66 ,  C12P21/00

Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites with unique specificity. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. Such molecules and/or compounds or combinations of such molecules and/or compounds can also be bound through recombination to various structures or supports according to the invention.

Abstract translation: 本发明提供了用于重组克隆的组合物和方法。 组合物包括具有独特特异性的多个重组位点的载体。 该方法允许同时克隆两种或更多种不同的核酸分子。 在一些实施方案中，分子融合在一起，而在其它实施方案中，分子被插入载体中的不同位点。 本发明还通常提供通过重组连接或连接许多可以相同或不同的分子和/或化合物（例如，化合物，药物，蛋白质或肽，脂质，核酸，碳水化合物等）。 这样的分子和/或化合物或这些分子和/或化合物的组合也可以通过重组与根据本发明的各种结构或支持物结合。