公开(公告)号：US20140178888A1

公开(公告)日：2014-06-26

申请号：US14191109

申请日：2014-02-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander VLASSOV ,  Mu LI ,  Emily ZERINGER ,  Richard CONRAD

IPC: C12Q1/68

CPC classification number: G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

Abstract translation: 公开了用于从生物流体和组织分离外来体的方法，组合物和试剂盒。 体积排除聚合物用于从生物样品中沉淀外来体，从而通过低速（台式）离心或过滤来进行外来体分离。 还描述了沉淀后进一步分离外来体。

公开(公告)号：US20180195057A1

公开(公告)日：2018-07-12

申请号：US15909789

申请日：2018-03-01

Applicant: Life Technologies Corporation

Inventor： Alexander VLASSOV ,  Sarah LAROCCA ,  Mu LI

IPC: C12N15/10 ,  C12Q1/6806 ,  G01N33/574 ,  G01N33/569

Abstract: Compositions and methods for nucleic acid isolation from an environmental or biological sample comprising nucleic acid-analysis interferents, particularly from microbiome-containing samples, are provided.

公开(公告)号：US20160320273A1

公开(公告)日：2016-11-03

申请号：US15142143

申请日：2016-04-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander VLASSOV ,  Mu LI ,  Emily ZERINGER ,  Richard CONRAD

IPC: G01N1/34 ,  G01N33/50 ,  C12Q1/68

CPC classification number: G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

公开(公告)号：US20150104801A1

公开(公告)日：2015-04-16

申请号：US14523764

申请日：2014-10-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander VLASSOV ,  Mu LI ,  Emily ZERINGER ,  Richard CONRAD

IPC: C12Q1/68 ,  G01N1/34

CPC classification number: G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

Abstract translation: 公开了用于从生物流体和组织分离外来体的方法，组合物和试剂盒。 体积排除聚合物用于从生物样品中沉淀外来体，从而通过低速（台式）离心或过滤来进行外来体分离。 还描述了沉淀后进一步分离外来体。