公开(公告)号：US20160102304A1

公开(公告)日：2016-04-14

申请号：US14886682

申请日：2015-10-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Richard CONRAD

IPC: C12N15/10

CPC classification number: C12N15/1006 ,  C07H1/08 ,  C12N15/1003

Abstract: The present invention concerns the use of methods and compositions for the isolation of small RNA molecules (100 nucleotides or fewer), such as microRNA and siRNA molecules. Such molecules are routinely lost in commonly used isolation procedures and therefore the present invention allows for a much higher level of enrichment or isolation of these small RNA molecules.

公开(公告)号：US20130245245A1

公开(公告)日：2013-09-19

申请号：US13778952

申请日：2013-02-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Richard CONRAD

IPC: C07H1/08

CPC classification number: C12N15/1006 ,  C07H1/08 ,  C12N15/1003

Abstract: The present invention concerns the use of methods and compositions for the isolation of small RNA molecules (100 nucleotides or fewer), such as microRNA and siRNA molecules. Such molecules are routinely lost in commonly used isolation procedures and therefore the present invention allows for a much higher level of enrichment or isolation of these small RNA molecules.

Abstract translation: 本发明涉及用于分离小RNA分子（100个核苷酸或更少）的方法和组合物，例如微小RNA和siRNA分子。 在通常使用的分离方法中常规地损失这样的分子，因此本发明允许这些小RNA分子的富集或分离更高水平。

公开(公告)号：US20140178888A1

公开(公告)日：2014-06-26

申请号：US14191109

申请日：2014-02-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander VLASSOV ,  Mu LI ,  Emily ZERINGER ,  Richard CONRAD

IPC: C12Q1/68

CPC classification number: G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

Abstract translation: 公开了用于从生物流体和组织分离外来体的方法，组合物和试剂盒。 体积排除聚合物用于从生物样品中沉淀外来体，从而通过低速（台式）离心或过滤来进行外来体分离。 还描述了沉淀后进一步分离外来体。

公开(公告)号：US20180087048A1

公开(公告)日：2018-03-29

申请号：US15829469

申请日：2017-12-01

Applicant: Life Technologies Corporation

Inventor： Richard CONRAD ,  Marie Gonzalez ,  Emily ZERINGER

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1003 ,  C12Q1/6804 ,  C12Q2527/125 ,  C12Q2565/518

Abstract: Methods of directly isolating nucleic acid from an embedded biological sample are provided. An emulsified digest is generated in the presence of a thermostable protease, and an additive selected from an alkylene glycol, a poly(alkylene glycol), or a block copolymer having an average Mn of 76 to 2900, or a salt or derivative or combination thereof. Nucleic acid is isolated directly from the emulsified digest. The methods eliminate the use of organic solvents such as xylene in a deparaffinization step prior to isolating nucleic acids from paraffin-embedded samples, for example.

公开(公告)号：US20160320273A1

公开(公告)日：2016-11-03

申请号：US15142143

申请日：2016-04-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander VLASSOV ,  Mu LI ,  Emily ZERINGER ,  Richard CONRAD

IPC: G01N1/34 ,  G01N33/50 ,  C12Q1/68

CPC classification number: G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

公开(公告)号：US20150104801A1

公开(公告)日：2015-04-16

申请号：US14523764

申请日：2014-10-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander VLASSOV ,  Mu LI ,  Emily ZERINGER ,  Richard CONRAD

IPC: C12Q1/68 ,  G01N1/34

CPC classification number: G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

Abstract translation: 公开了用于从生物流体和组织分离外来体的方法，组合物和试剂盒。 体积排除聚合物用于从生物样品中沉淀外来体，从而通过低速（台式）离心或过滤来进行外来体分离。 还描述了沉淀后进一步分离外来体。