公开(公告)号：US20230313286A1

公开(公告)日：2023-10-05

申请号：US18173386

申请日：2023-02-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ioanna PAGANI ,  Emily ZERINGER ,  Kelly LI ,  Boli HUANG

IPC: C12Q1/686 ,  C12Q1/689 ,  C12Q1/6851 ,  C12Q1/70 ,  B01L3/00 ,  C12Q1/6806 ,  C12Q1/6848

CPC classification number: C12Q1/686 ,  C12Q1/689 ,  C12Q1/6851 ,  C12Q1/706 ,  B01L3/5085 ,  C12Q1/6806 ,  C12Q1/6848 ,  B01L2300/0819

Abstract: Nucleic acid reagents and corresponding methods of using the same for monitoring and evaluating nucleic acid extraction and amplification reactions.

公开(公告)号：US20140178888A1

公开(公告)日：2014-06-26

申请号：US14191109

申请日：2014-02-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander VLASSOV ,  Mu LI ,  Emily ZERINGER ,  Richard CONRAD

IPC: C12Q1/68

CPC classification number: G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

Abstract translation: 公开了用于从生物流体和组织分离外来体的方法，组合物和试剂盒。 体积排除聚合物用于从生物样品中沉淀外来体，从而通过低速（台式）离心或过滤来进行外来体分离。 还描述了沉淀后进一步分离外来体。

公开(公告)号：US20180087048A1

公开(公告)日：2018-03-29

申请号：US15829469

申请日：2017-12-01

Applicant: Life Technologies Corporation

Inventor： Richard CONRAD ,  Marie Gonzalez ,  Emily ZERINGER

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1003 ,  C12Q1/6804 ,  C12Q2527/125 ,  C12Q2565/518

Abstract: Methods of directly isolating nucleic acid from an embedded biological sample are provided. An emulsified digest is generated in the presence of a thermostable protease, and an additive selected from an alkylene glycol, a poly(alkylene glycol), or a block copolymer having an average Mn of 76 to 2900, or a salt or derivative or combination thereof. Nucleic acid is isolated directly from the emulsified digest. The methods eliminate the use of organic solvents such as xylene in a deparaffinization step prior to isolating nucleic acids from paraffin-embedded samples, for example.

公开(公告)号：US20180073055A1

公开(公告)日：2018-03-15

申请号：US15687046

申请日：2017-08-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ioanna PAGANI ,  Emily ZERINGER ,  Kelly LI ,  Boli HUANG

IPC: C12Q1/68 ,  B01L3/00

CPC classification number: C12Q1/686 ,  B01L3/5085 ,  B01L2300/0819 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/689 ,  C12Q1/706 ,  C12Q2537/143 ,  C12Q2545/101

Abstract: Nucleic acid reagents and corresponding methods of using the same for monitoring and evaluating nucleic acid extraction and amplification reactions.

公开(公告)号：US20160320273A1

公开(公告)日：2016-11-03

申请号：US15142143

申请日：2016-04-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander VLASSOV ,  Mu LI ,  Emily ZERINGER ,  Richard CONRAD

IPC: G01N1/34 ,  G01N33/50 ,  C12Q1/68

CPC classification number: G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

公开(公告)号：US20150104801A1

公开(公告)日：2015-04-16

申请号：US14523764

申请日：2014-10-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander VLASSOV ,  Mu LI ,  Emily ZERINGER ,  Richard CONRAD

IPC: C12Q1/68 ,  G01N1/34

CPC classification number: G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

Abstract translation: 公开了用于从生物流体和组织分离外来体的方法，组合物和试剂盒。 体积排除聚合物用于从生物样品中沉淀外来体，从而通过低速（台式）离心或过滤来进行外来体分离。 还描述了沉淀后进一步分离外来体。