公开(公告)号：US08497126B2

公开(公告)日：2013-07-30

申请号：US11824289

申请日：2007-06-29

申请人： Marie D. Bryhan ,  Paul E. Gagnon, Jr. ,  Zhong-he Shen ,  Oliva V. LaChance ,  Hongming Wang

发明人： Marie D. Bryhan ,  Paul E. Gagnon, Jr. ,  Zhong-he Shen ,  Oliva V. LaChance ,  Hongming Wang

IPC分类号： C12N5/02

CPC分类号： B29C59/14 ,  B29C59/142 ,  C12N5/0068 ,  C12N2533/30

摘要： A method of producing an improved cell growth surface and cell attachment surface. According to the present invention, a polymer article is molded at temperature in excess of 550° F. at the injection tip. After allowing the part to cool, a stream of plasma comprised of activated gaseous species generated by a microwave source is imparted on the article. This stream is directed at the surface of a polymer substrate in a controlled fashion such that the surface is imparted with attributes for cell adhesion superior to those of untreated polymer or polymer treated by other methods.

摘要翻译： 一种生产改良的细胞生长表面和细胞附着表面的方法。 根据本发明，聚合物制品在注射尖端处的模制温度超过550°F。 在使部件冷却之后，将由微波源产生的活化气态物质组成的等离子体流送到制品上。 该流以受控的方式指向聚合物基材的表面，使得表面具有优于未经处理的聚合物或通过其它方法处理的聚合物的细胞粘附的特性。

公开(公告)号：US07923241B2

公开(公告)日：2011-04-12

申请号：US11973832

申请日：2007-10-10

申请人： Ye Fang ,  Paul E. Gagnon, Jr. ,  Terry T. Goodrich ,  Joydeep Lahiri ,  John S. Peanasky ,  Hongming Wang ,  Jun Xi

发明人： Ye Fang ,  Paul E. Gagnon, Jr. ,  Terry T. Goodrich ,  Joydeep Lahiri ,  John S. Peanasky ,  Hongming Wang ,  Jun Xi

IPC分类号： C12M3/04 ,  G01N33/53

CPC分类号： C12M23/20 ,  C12M25/06

摘要： Disclosed is a cell culture article including: a substrate; a tie-layer attached to at least the substrate; and a bio-compatible layer attached to at least the tie layer, the bio-compatible layer having been obtained from surface oxidation of a polymer layer. Also disclosed are methods for making the cell culture article and methods for performing an assay of a ligand with the article.

摘要翻译： 公开了一种细胞培养物品，其特征在于，包括： 连接至少至少衬底的连接层; 以及连接到至少所述粘结层的生物相容层，所述生物相容层已经从聚合物层的表面氧化获得。 还公开了制备细胞培养物品的方法和用该物品进行配体测定的方法。