公开(公告)号：US08759013B2

公开(公告)日：2014-06-24

申请号：US13648708

申请日：2012-10-10

Applicant: Ye Fang ,  Anthony Glenn Frutos ,  David Harry Randle ,  Elizabeth Tran

Inventor： Ye Fang ,  Anthony Glenn Frutos ,  David Harry Randle ,  Elizabeth Tran

IPC: G01N33/53

CPC classification number: G01N33/542 ,  G01N21/7743 ,  G01N33/566 ,  G01N2500/10

Abstract: A method and apparatus, as defined herein, for use in compound screening, compound profiling, or both assays, for example, against two different cellular targets in, for example, a single cell-type.

Abstract translation: 如本文所定义的用于化合物筛选，化合物分析或两种测定的方法和装置，例如针对例如单细胞型的两种不同的细胞靶标。

公开(公告)号：US20120289432A1

公开(公告)日：2012-11-15

申请号：US13519271

申请日：2010-12-16

Applicant: Huayun Deng ,  Ye Fang ,  Ann MeeJin Ferrie ,  Haiyan Sun ,  Elizabeth Tran

Inventor： Huayun Deng ,  Ye Fang ,  Ann MeeJin Ferrie ,  Haiyan Sun ,  Elizabeth Tran

IPC: C12Q1/48 ,  C40B30/06 ,  G01N21/41

CPC classification number: G01N33/5041 ,  G01N33/54373 ,  G01N2333/91215

Abstract: Disclosed are methods to characterize PI3K inhibitors and Rho kinase inhibitors using label-free cellular assays. Disclosed are also methods to characterize a cell whether it has a deregulated PI3K pathway or not.

Abstract translation: 公开了使用无标记细胞测定来表征PI3K抑制剂和Rho激酶抑制剂的方法。 还公开了表征细胞是否具有失去PI3K通路的方法。

公开(公告)号：US08293484B2

公开(公告)日：2012-10-23

申请号：US12893389

申请日：2010-09-29

Applicant: Ye Fang ,  Fang Lai ,  Laurent A. G. Picard ,  Brian L. Webb

Inventor： Ye Fang ,  Fang Lai ,  Laurent A. G. Picard ,  Brian L. Webb

IPC: G01N33/53

CPC classification number: C12N5/0068 ,  C12N2503/00 ,  C12N2535/10

Abstract: Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.

公开(公告)号：US20120083428A1

公开(公告)日：2012-04-05

申请号：US12893389

申请日：2010-09-29

Applicant: Ye Fang ,  Fang Lai ,  Laurent A.G. Picard ,  Brian L. Webb

Inventor： Ye Fang ,  Fang Lai ,  Laurent A.G. Picard ,  Brian L. Webb

IPC: C40B40/02 ,  C40B50/06

CPC classification number: C12N5/0068 ,  C12N2503/00 ,  C12N2535/10

Abstract: Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.

公开(公告)号：US20120064519A1

公开(公告)日：2012-03-15

申请号：US13300029

申请日：2011-11-18

Applicant: Ye Fang ,  Ann M. Ferrie ,  Joydeep Lahiri ,  Deepti J. Mudaliar ,  Qi Wu

Inventor： Ye Fang ,  Ann M. Ferrie ,  Joydeep Lahiri ,  Deepti J. Mudaliar ,  Qi Wu

IPC: C12Q1/68 ,  G01N33/566 ,  C12Q1/02

CPC classification number: G01N21/648 ,  G01N21/7743

Abstract: A system and method as defined herein for dual-detection of evanescent-wave label-free light and evanescent-wave excited-fluorescent label-emitted light in an optical biosensor.

Abstract translation: 本文所定义的系统和方法，用于在光学生物传感器中双重检测ev逝波无标记光和ev逝波激发荧光标记发射光。

公开(公告)号：US08129198B2

公开(公告)日：2012-03-06

申请号：US12854588

申请日：2010-08-11

Applicant: Ye Fang ,  Ann M. Ferrie ,  Yulong Hong ,  Brian L. Webb

Inventor： Ye Fang ,  Ann M. Ferrie ,  Yulong Hong ,  Brian L. Webb

IPC: G01N33/543

CPC classification number: B01L3/5085 ,  B01L2200/12 ,  B01L2300/069 ,  B01L2300/0829 ,  Y10S435/81 ,  Y10S436/809 ,  Y10T29/49885

Abstract: A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three-dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.

Abstract translation: 公开了适用于生物或化学测定的底物板或装置。 该装置可以采用具有三维多孔层的多孔板的形式，作为每个孔内的支撑表面的一部分，用于固定探针物质。 多孔层的特征在于具有由连续的固体材料的基体限定的多个相互连接的空隙。 还描述了一种方法及其变体。

公开(公告)号：US20120015846A1

公开(公告)日：2012-01-19

申请号：US13181051

申请日：2011-07-12

Applicant: Ye Fang

Inventor： Ye Fang

IPC: C40B30/06 ,  C12Q1/02

CPC classification number: G01N33/5014 ,  G01N33/54373 ,  G01N2333/70571

Abstract: Described are methods relating to assessing antitargets of molecules. Also described are methods of screening molecules. In some aspects of the methods, the molecules are analyzed using a label free biosensor.

Abstract translation: 描述了评估分子抗靶物的方法。 还描述了筛选分子的方法。 在该方法的某些方面，使用无标记的生物传感器分析分子。

公开(公告)号：US20100184626A1

公开(公告)日：2010-07-22

申请号：US12696622

申请日：2010-01-29

Applicant: Ye Fang ,  Anthony G. Frutos ,  Steven J. Jonas ,  Peter J. Kalal ,  Joydeep Lahiri

Inventor： Ye Fang ,  Anthony G. Frutos ,  Steven J. Jonas ,  Peter J. Kalal ,  Joydeep Lahiri

IPC: C40B40/10

CPC classification number: B01J19/0046 ,  B01J2219/00385 ,  B01J2219/00387 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00707 ,  B01J2219/00725 ,  B01J2219/0074 ,  B82Y15/00 ,  B82Y30/00 ,  C07K1/047 ,  C40B40/10 ,  C40B60/14 ,  G01N33/5438 ,  G01N33/566 ,  G01N33/6845 ,  G01N33/6872 ,  G01N33/74 ,  G01N2333/726 ,  G01N2500/02

Abstract: The present invention overcomes the problems and disadvantages associated with prior art arrays by providing an array comprising a plurality of biological membrane microspots associated with a surface of a substrate that can be produced, used and stored, not in an aqueous environment, but in an environment exposed to air under ambient or controlled humidities. Preferably, the biological membrane microspots comprise a membrane bound protein. Most preferably, the membrane bound protein is a G-protein coupled receptor, an ion channel, a receptor serine/threonine kinase or a receptor tyrosine kinase.

Abstract translation: 本发明克服了与现有技术阵列相关的问题和缺点，提供了一种阵列，其包括与基底表面相关联的多个生物膜微孔，该生物膜微孔可以在水溶液环境中生产，使用和储存，而不是在环境中 在环境或受控的湿度下暴露于空气。 优选地，生物膜微点包含膜结合蛋白。 最优选地，膜结合蛋白是G蛋白偶联受体，离子通道，受体丝氨酸/苏氨酸激酶或受体酪氨酸激酶。

公开(公告)号：US20090298116A1

公开(公告)日：2009-12-03

申请号：US12471835

申请日：2009-05-26

Applicant: Ye Fang ,  Jianguo Wang ,  Ying Zhang

Inventor： Ye Fang ,  Jianguo Wang ,  Ying Zhang

IPC: C12Q1/02 ,  C12M1/00 ,  G03F7/20

CPC classification number: C12M23/16 ,  B01L3/5085 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/163 ,  B81B2201/06 ,  B81B2203/0346 ,  B81B2203/0376 ,  B81C99/0085 ,  B81C99/009 ,  C12M23/12

Abstract: A cell culture apparatus includes a substrate having formed therein a micro-well array, the micro-well array comprising a plurality of micro-wells. Each micro-well is defined by a curved surface which is concave.

Abstract translation: 细胞培养装置包括其中形成有微孔阵列的基底，微孔阵列包括多个微孔。 每个微孔由凹入的曲面限定。

公开(公告)号：US20090275480A1

公开(公告)日：2009-11-05

申请号：US12492732

申请日：2009-06-26

Applicant: Ye Fang

Inventor： Ye Fang

IPC: C40B30/02 ,  C40B30/04

CPC classification number: C40B30/04 ,  B01J2219/00315 ,  B01J2219/00387 ,  B01J2219/00497 ,  B01J2219/00511 ,  B01J2219/00536 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00639 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00693 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B01J2219/0074 ,  B82Y30/00 ,  C12Q1/6837 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B60/14 ,  G01N2035/00158 ,  Y10S436/807 ,  C12Q2537/161

Abstract: A method and apparatus for implementing the method is provided. The method involves performing an indirect competitive binding assay on a microarray to identify biological or chemical targets and screen for compounds of interest. The microarray comprises a common ligand located among membrane-, lipid- or protein-associated active binding sites. The method takes advantage of known or well-characterized binding kinetics, and steric interference between biological or chemicals targets of interest and universal readout units for different binding sites within the limited confines of a microspot. The biological targets, chemicals or organisms can specifically bind to target-binding sites, while the universal readout unit binds to the ligands in the microspot.

Abstract translation: 提供了一种用于实现该方法的方法和装置。 该方法包括在微阵列上进行间接竞争性结合测定以鉴定生物或化学靶标并筛选感兴趣的化合物。 微阵列包含位于膜 - ，脂质 - 或蛋白质相关的活性结合位点之间的共同配体。 该方法利用已知或良好表征的结合动力学，以及感兴趣的生物学或化学物质目标之间的空间干扰以及在微量有限范围内的不同结合位点的通用读出单元。 生物靶标，化学物质或生物体可以特异性地结合靶结合位点，而通用读出单元与微孔中的配体结合。