公开(公告)号：US20050026240A1

公开(公告)日：2005-02-03

申请号：US10896338

申请日：2004-07-21

申请人： Michael Sommer ,  Pasquale Degiorgio ,  Bronislaw Czech ,  Gena Fischer ,  E. Chapman ,  David Zelmanovic ,  Jolanta Kunicka

发明人： Michael Sommer ,  Pasquale Degiorgio ,  Bronislaw Czech ,  Gena Fischer ,  E. Chapman ,  David Zelmanovic ,  Jolanta Kunicka

IPC分类号： G01N33/49 ,  G01N1/30 ,  G01N15/14 ,  G01N33/48 ,  G01N33/50

CPC分类号： G01N33/5002 ,  G01N2015/1486 ,  G01N2015/1488 ,  Y10T436/101666 ,  Y10T436/107497

摘要： The enumeration and analysis of residual white blood cells in a sample of leukocyte-reduced blood products is conducted by forming a suspension of the leukocyte-reduced blood products with a sufficient amount of a lysing reagent. The lysing reagent comprises a buffer with a low molar concentration, and a non-ionic surfactant. The suspension of leukocyte-reduced blood products and the lysing reagent is incubated for a sufficient time at a temperature sufficient to selectively lyse the platelets and red blood cells without damaging the white blood cells. The white blood cells of the lysed blood products are then contacted with a suitable dye to stain the white blood cells and the number of stained white blood cells is measured. The lysing reagent is free of harsh organic solvents which can damage the plastic components of automated clinical analyzers.

摘要翻译： 通过用足够量的裂解试剂形成白细胞减少的血液制品的悬浮液来进行白细胞减少的血液制品样品中的残余白细胞的计数和分析。 裂解试剂包含具有低摩尔浓度的缓冲液和非离子表面活性剂。 将白细胞减少的血液制品和裂解试剂的悬浮液在足以在不损伤白细胞的情况下足以选择性地裂解血小板和红细胞的温度下孵育足够的时间。 然后将裂解的血液制品的白细胞与合适的染料接触以染色白细胞，并测量染色的白细胞数。 裂解试剂不含苛刻的有机溶剂，可能会损坏自动化临床分析仪的塑料部件。