公开(公告)号：US20050026240A1

公开(公告)日：2005-02-03

申请号：US10896338

申请日：2004-07-21

申请人： Michael Sommer ,  Pasquale Degiorgio ,  Bronislaw Czech ,  Gena Fischer ,  E. Chapman ,  David Zelmanovic ,  Jolanta Kunicka

发明人： Michael Sommer ,  Pasquale Degiorgio ,  Bronislaw Czech ,  Gena Fischer ,  E. Chapman ,  David Zelmanovic ,  Jolanta Kunicka

IPC分类号： G01N33/49 ,  G01N1/30 ,  G01N15/14 ,  G01N33/48 ,  G01N33/50

CPC分类号： G01N33/5002 ,  G01N2015/1486 ,  G01N2015/1488 ,  Y10T436/101666 ,  Y10T436/107497

摘要： The enumeration and analysis of residual white blood cells in a sample of leukocyte-reduced blood products is conducted by forming a suspension of the leukocyte-reduced blood products with a sufficient amount of a lysing reagent. The lysing reagent comprises a buffer with a low molar concentration, and a non-ionic surfactant. The suspension of leukocyte-reduced blood products and the lysing reagent is incubated for a sufficient time at a temperature sufficient to selectively lyse the platelets and red blood cells without damaging the white blood cells. The white blood cells of the lysed blood products are then contacted with a suitable dye to stain the white blood cells and the number of stained white blood cells is measured. The lysing reagent is free of harsh organic solvents which can damage the plastic components of automated clinical analyzers.

摘要翻译： 通过用足够量的裂解试剂形成白细胞减少的血液制品的悬浮液来进行白细胞减少的血液制品样品中的残余白细胞的计数和分析。 裂解试剂包含具有低摩尔浓度的缓冲液和非离子表面活性剂。 将白细胞减少的血液制品和裂解试剂的悬浮液在足以在不损伤白细胞的情况下足以选择性地裂解血小板和红细胞的温度下孵育足够的时间。 然后将裂解的血液制品的白细胞与合适的染料接触以染色白细胞，并测量染色的白细胞数。 裂解试剂不含苛刻的有机溶剂，可能会损坏自动化临床分析仪的塑料部件。

公开(公告)号：US07745219B2

公开(公告)日：2010-06-29

申请号：US10896338

申请日：2004-07-21

申请人： Michael J. Sommer ,  Pasquale Degiorgio ,  Bronislaw P. Czech ,  Gena Fischer ,  E. Sabrinah Chapman ,  David Zelmanovic ,  Jolanta Kunicka

发明人： Michael J. Sommer ,  Pasquale Degiorgio ,  Bronislaw P. Czech ,  Gena Fischer ,  E. Sabrinah Chapman ,  David Zelmanovic ,  Jolanta Kunicka

IPC分类号： G01N33/48 ,  G01N33/49

CPC分类号： G01N33/5002 ,  G01N2015/1486 ,  G01N2015/1488 ,  Y10T436/101666 ,  Y10T436/107497

摘要： The enumeration and analysis of residual white blood cells in a sample of leukocyte-reduced blood products is conducted by forming a suspension of the leukocyte-reduced blood products with a sufficient amount of a lysing reagent. The lysing reagent comprises a buffer with a low molar concentration, and a non-ionic surfactant. The suspension of leukocyte-reduced blood products and the lysing reagent is incubated for a sufficient time at a temperature sufficient to selectively lyse the platelets and red blood cells without damaging the white blood cells. The white blood cells of the lysed blood products are then contacted with a suitable dye to stain the white blood cells and the number of stained white blood cells is measured. The lysing reagent is free of harsh organic solvents which can damage the plastic components of automated clinical analyzers.

摘要翻译： 通过用足够量的裂解试剂形成白细胞减少的血液制品的悬浮液来进行白细胞减少的血液制品样品中的残余白细胞的计数和分析。 裂解试剂包含具有低摩尔浓度的缓冲液和非离子表面活性剂。 将白细胞减少的血液制品和裂解试剂的悬浮液在足以在不损伤白细胞的情况下足以选择性地裂解血小板和红细胞的温度下孵育足够的时间。 然后将裂解的血液制品的白细胞与合适的染料接触以染色白细胞，并测量染色的白细胞数。 裂解试剂不含苛刻的有机溶剂，可能会损坏自动化临床分析仪的塑料部件。

公开(公告)号：US07670798B2

公开(公告)日：2010-03-02

申请号：US10442706

申请日：2003-05-20

申请人： Leonard Ornstein ,  Gena Fischer ,  David Zelmanovic ,  Pamela Elsins ,  Jolanta Kunicka ,  Michael J. Malin

发明人： Leonard Ornstein ,  Gena Fischer ,  David Zelmanovic ,  Pamela Elsins ,  Jolanta Kunicka ,  Michael J. Malin

IPC分类号： G01N1/00 ,  G01N7/00 ,  G01N31/00 ,  G01N33/48 ,  G01N33/546

CPC分类号： G01N33/96 ,  A01N1/00 ,  G01N15/1459 ,  G01N2015/0073 ,  G01N2015/1486 ,  Y10T436/10 ,  Y10T436/11 ,  Y10T436/2525

摘要： The present invention describes semi- and fully-automated methods and reagents therefor for the assay and analysis of body fluid samples, particularly non-blood samples. The methods and reagents are especially useful for the assay and analysis of cerebrospinal fluid (CSF) samples. The reagent compositions sphere and fix all cells in the sample in suspension. Reported results can include red blood cell (RBC) and white blood cell (WBC) counts, WBC differential values, cell-by-cell volumes and dry-mass concentrations.

摘要翻译： 本发明描述了用于体液样品，特别是非血液样品的测定和分析的半自动和全自动化方法和试剂。 方法和试剂对于脑脊液（CSF）样品的测定和分析特别有用。 试剂组合物球体并将悬浮液中的样品中的所有细胞固定。 报告的结果可以包括红细胞（RBC）和白血球（WBC）计数，WBC差异值，逐个细胞体积和干质量浓度。

公开(公告)号：US06285377B1

公开(公告)日：2001-09-04

申请号：US08882974

申请日：1997-06-26

申请人： Albert Greenbaum ,  Martin Sorette ,  Jolanta Kunicka ,  Gena Fischer

发明人： Albert Greenbaum ,  Martin Sorette ,  Jolanta Kunicka ,  Gena Fischer

IPC分类号： G06T1120

CPC分类号： G06T11/206

摘要： A system and method for displaying parameters in a smoothed normalized star diagram where parameters are received from a medical laboratory instrument and processed. The processing includes calculating a normalized parameter value for each parameter using a predetermined mean and standard deviation and scaling the resulting normalized parameter value using a range restricting function. Each scaled parameter is assigned to a spoke and rays are drawn between adjacent spokes at a small angular interval. The ends of the rays are then joined by line segments, thereby constructing a smooth overall diagram.

摘要翻译： 一种用于在平滑的归一化星图中显示参数的系统和方法，其中从医学实验室仪器接收参数并进行处理。 该处理包括使用预定的平均值和标准偏差计算每个参数的归一化参数值，并使用范围限制函数对所得到的归一化参数值进行缩放。 每个缩放的参数被分配给辐条，并且在相邻辐条之间以小的角度间隔绘制光线。 然后射线的端部通过线段连接，从而构成平滑的总体图。