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1.发明申请公开(公告)号：US20200157607A1
公开(公告)日：2020-05-21
申请号：US16782794
申请日：2020-02-05
申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY , KYORIN PHARMACEUTICAL CO., LTD.
摘要： The present invention provides a reciprocal-flow-type nucleic acid amplification device comprising: heaters capable of forming a denaturation temperature zone and an extension/annealing temperature zone; a fluorescence detector capable of detecting movement of a sample solution between the two temperature zones; a pair of liquid delivery mechanisms that allow the sample solution to move between the two temperature zones and that are configured to be open to atmospheric pressure when liquid delivery stops; a substrate on which the chip for nucleic acid amplification according to claim 2 can be placed; and a control mechanism that controls driving of each liquid delivery mechanism by receiving an electrical signal from the fluorescence detector relating to movement of the sample solution from the control mechanism; the device being capable of performing real-time PCR by measuring fluorescence intensity for each thermal cycle.
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公开(公告)号：US20210187510A1
公开(公告)日：2021-06-24
申请号：US17270848
申请日：2019-08-29
申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY , KYORIN PHARMACEUTICAL CO., LTD.
发明人： Hidenori NAGAI , Shunsuke FURUTANI , Hideyasu KUBO
摘要： A PCR vessel having: a substrate, a flow channel formed in the substrate, a pair of filters provided at both ends of the flow channel, a pair of air communication ports communicating with the flow channel through the filters, a thermal cycle region formed between the pair of filters in the flow channel, and a sample injection port through which a sample can be injected into the flow channel from above; wherein the sample injection port in the surface of the substrate has an area of 0.7 to 1.8 mm2.
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3.发明申请公开(公告)号：US20170130261A1
公开(公告)日：2017-05-11
申请号：US15322000
申请日：2015-07-07
摘要： The present invention provides a reciprocal-flow-type nucleic acid amplification device comprising:heaters capable of forming a denaturation temperature zone and an extension/annealing temperature zone; a fluorescence detector capable of detecting movement of a sample solution between the two temperature zones; a pair of liquid delivery mechanisms that allow the sample solution to move between the two temperature zones and that are configured to be open to atmospheric pressure when liquid delivery stops; a substrate on which the chip for nucleic acid amplification according to claim 2 can be placed; and a control mechanism that controls driving of each liquid delivery mechanism by receiving an electrical signal from the fluorescence detector relating to movement of the sample solution from the control mechanism; the device being capable of performing real-time PCR by measuring fluorescence intensity for each thermal cycle.
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