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1.发明申请公开(公告)号：US20200157607A1
公开(公告)日：2020-05-21
申请号：US16782794
申请日：2020-02-05
申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY , KYORIN PHARMACEUTICAL CO., LTD.
摘要： The present invention provides a reciprocal-flow-type nucleic acid amplification device comprising: heaters capable of forming a denaturation temperature zone and an extension/annealing temperature zone; a fluorescence detector capable of detecting movement of a sample solution between the two temperature zones; a pair of liquid delivery mechanisms that allow the sample solution to move between the two temperature zones and that are configured to be open to atmospheric pressure when liquid delivery stops; a substrate on which the chip for nucleic acid amplification according to claim 2 can be placed; and a control mechanism that controls driving of each liquid delivery mechanism by receiving an electrical signal from the fluorescence detector relating to movement of the sample solution from the control mechanism; the device being capable of performing real-time PCR by measuring fluorescence intensity for each thermal cycle.
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2.发明申请公开(公告)号：US20170130261A1
公开(公告)日：2017-05-11
申请号：US15322000
申请日：2015-07-07
摘要： The present invention provides a reciprocal-flow-type nucleic acid amplification device comprising:heaters capable of forming a denaturation temperature zone and an extension/annealing temperature zone; a fluorescence detector capable of detecting movement of a sample solution between the two temperature zones; a pair of liquid delivery mechanisms that allow the sample solution to move between the two temperature zones and that are configured to be open to atmospheric pressure when liquid delivery stops; a substrate on which the chip for nucleic acid amplification according to claim 2 can be placed; and a control mechanism that controls driving of each liquid delivery mechanism by receiving an electrical signal from the fluorescence detector relating to movement of the sample solution from the control mechanism; the device being capable of performing real-time PCR by measuring fluorescence intensity for each thermal cycle.
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公开(公告)号：US20210170398A1
公开(公告)日：2021-06-10
申请号：US17269876
申请日：2019-08-29
发明人： Yusuke FUCHIWAKI
摘要： An assay device allows enhancement of the liquid control performance. The assay device of the present invention includes a microflow passage 1,31,41 which allows flow of the liquid, an absorbing porous medium 2,42 disposed at a distance from one end of the microflow passage, and a separating space 3,43 disposed between the one end of the microflow passage and the absorbing porous medium. The assay device further includes two sideways ventilation passages 6,46 which are adjacent to both sides of the microflow passage, respectively in the width direction orthogonal to the flow direction, the two sideways ventilation passages 6,46 being communicated with the microflow passage to allow air circulation.
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公开(公告)号：US20240342705A1
公开(公告)日：2024-10-17
申请号：US18259645
申请日：2021-12-27
CPC分类号： B01L3/502707 , G01N35/08 , B01L2300/048 , B01L2300/069 , B01L2300/0867
摘要： An assay device allows reducing manufacturing variation, maintaining the measurement accuracy of the assay device at a high level, and enhancing the liquid control performance. The assay device of the present invention includes a microfluidic channel 2 allowing liquid to flow, an absorbing porous medium 3 disposed at a distance from one end 2a of the microfluidic channel 2, a separating space 4 disposed between the microfluidic channel 2 and the absorbing porous medium 3, and a housing space 5 housing the absorbing porous medium 3. The assay device further includes a lower member 20 which is an integrally molded article constituting a part of the assay device. The lower member 20 defines a lower portion 2b of the microfluidic channel 2, a lower portion 4a of the separating space 4, and a lower portion 5a of the housing space 5. The lower portions 4a, 5a of the separating space 4 and the housing space 5 are inclined downward toward one side from the other side in a flow direction. The lower member 20 supports the absorbing porous medium 3 at the lower portion 5a of the housing space 5.
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公开(公告)号：US20240091771A1
公开(公告)日：2024-03-21
申请号：US18246143
申请日：2021-09-17
发明人： Yusuke FUCHIWAKI , Masato TANAKA , Shohei YAMAMURA , Naoki MORISHITA , Kumiko KAMIYA , Seiichiro MATSUZAKI
IPC分类号： B01L3/00
CPC分类号： B01L3/502723 , B01L2300/048 , B01L2300/069
摘要： An assay device and an assay method are each capable of ensuring the accuracy of a target substance detection section. The assay device includes a plurality of assay units 100, each assay unit 100 including a microfluidic channel configured to allow a liquid to flow; a porous absorbing medium disposed at a distance from one end of the microfluidic channel, the one end being located on one side in a flow direction of the liquid; and a separation space disposed between the one end of the microfluidic channel and the porous absorbing medium, in which the microfluidic channel includes, in the microfluidic channel, a detection section 14 having immobilized thereon a substance capable of specifically reacting with a target substance, and an internal standard section 54 having immobilized thereon an internal standard substance, and each assay unit includes two parallel ventilation passages that are respectively adjacent to both sides of the microfluidic channel in the width direction orthogonal to the flow direction, the two parallel ventilation passages communicating with the microfluidic channel to allow for air circulation.
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