-
公开(公告)号：US20210187510A1
公开(公告)日：2021-06-24
申请号：US17270848
申请日：2019-08-29
Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY , KYORIN PHARMACEUTICAL CO., LTD.
Inventor： Hidenori NAGAI , Shunsuke FURUTANI , Hideyasu KUBO
Abstract: A PCR vessel having: a substrate, a flow channel formed in the substrate, a pair of filters provided at both ends of the flow channel, a pair of air communication ports communicating with the flow channel through the filters, a thermal cycle region formed between the pair of filters in the flow channel, and a sample injection port through which a sample can be injected into the flow channel from above; wherein the sample injection port in the surface of the substrate has an area of 0.7 to 1.8 mm2.
-
2.发明申请公开(公告)号：US20170130261A1
公开(公告)日：2017-05-11
申请号：US15322000
申请日：2015-07-07
Inventor： Hidenori NAGAI , Shunsuke FURUTANI , Yoshihisa HAGIHARA , Yusuke FUCHIWAKI
Abstract: The present invention provides a reciprocal-flow-type nucleic acid amplification device comprising:heaters capable of forming a denaturation temperature zone and an extension/annealing temperature zone; a fluorescence detector capable of detecting movement of a sample solution between the two temperature zones; a pair of liquid delivery mechanisms that allow the sample solution to move between the two temperature zones and that are configured to be open to atmospheric pressure when liquid delivery stops; a substrate on which the chip for nucleic acid amplification according to claim 2 can be placed; and a control mechanism that controls driving of each liquid delivery mechanism by receiving an electrical signal from the fluorescence detector relating to movement of the sample solution from the control mechanism; the device being capable of performing real-time PCR by measuring fluorescence intensity for each thermal cycle.
-
公开(公告)号：US20220145360A1
公开(公告)日：2022-05-12
申请号：US17438012
申请日：2020-03-13
Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY , KYORIN PHARMACEUTICAL CO., LTD.
Inventor： Hidenori NAGAI , Satoru IWANAMI
IPC: C12Q1/686
Abstract: The invention provides a reciprocal-flow-type nucleic acid amplification method performing thermal cycling by reciprocating a sample liquid between a denaturation temperature zone and an elongation-annealing temperature zone with a connected microchannel including at least a curved channel corresponding to the denaturation temperature zone, a curved channel corresponding to the elongation-annealing temperature zone, a linear or curved intermediate channel that connects the aforementioned curved channels, and a connector to connect to a liquid delivery mechanism for enabling movement of the sample liquid. The method includes moving the sample liquid in the channel by the liquid delivery mechanism that is open to atmospheric pressure when liquid delivery is stopped, and measuring a fluorescence intensity for each thermal cycle at a predetermined point on the channel corresponding to the denaturation temperature zone and at a predetermined point on the channel corresponding to the elongation-annealing temperature zone to perform real-time PCR.
-
公开(公告)号：US20210207205A1
公开(公告)日：2021-07-08
申请号：US17209602
申请日：2021-03-23
Applicant: Nippon Sheet Glass Company, Limited , NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY , Go!Foton, Inc.
Inventor： Takashi FUKUZAWA , Hidenori NAGAI , Naofumi NISHIZAWA
Abstract: A PCR reaction vessel includes: a substrate; a channel formed on the substrate; a pair of filters, a first filter and a second filter, provided at respective ends of the channel; a pair of air communication ports, a first air communication port and a second air communication port, that communicate with the channel through the first filter and the second filter; a thermal cycle region formed between the first filter and the second filter in the channel; a branch point formed between the first filter and the second filter in the channel; a branched channel whose one end is connected to the branch point; and a sample introduction port formed at the other end of the branched channel.
-
Patent Agency Ranking
5.发明申请公开(公告)号：US20240408595A1
公开(公告)日：2024-12-12
申请号：US18702256
申请日：2022-10-18
Applicant: ASAHI KASEI KABUSHIKI KAISHA , NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
Inventor： Yuzo KOTANI , Ryo IINO , Kanako MIZUMURA , Hidenori NAGAI , Syunsuke FURUTANI
IPC: B01L3/00 , B32B3/30 , B32B17/10 , B32B27/08 , B32B27/30 , B32B37/24 , B32B38/00 , B32B38/10 , G03F7/16 , G03F7/20 , G03F7/40
Abstract: A structure 10 comprising microchannels 14, wherein: the structure 10 includes a base material 11, a partition material 12 and a cover material 13, at least portions of the base material 11 and partition material 12 have a resin region obtained from an alkali-soluble resin 21, in the infrared absorption spectrum by infrared spectroscopy of the resin region 21, the ratio (Aa/Ac) between: the maximum peak intensity (Aa) in a first range of 1555 to 1575 cm−1 and the maximum peak intensity (Ac) in a second range of 1715 to 1735 cm−1 is greater than 0 and 0.200 or lower, and the water contact angle on the flow channel-forming surface 11a of the base material 11 is 40 to 150 degrees.
-
公开(公告)号：US20180311673A1
公开(公告)日：2018-11-01
申请号：US16025398
申请日：2018-07-02
Applicant: Nippon Sheet Glass Company, Limited , National Institute of Advanced Industrial Science and Technology
Inventor： Takashi FUKUZAWA , Hidenori NAGAI
IPC: B01L7/00 , B01L3/00 , B01L3/02 , C12Q1/6806
Abstract: A reaction processor includes: a reaction processing vessel placing portion for placing a reaction processing vessel provided with a channel into which a sample is introduced; a temperature control system, which controls the temperature of the channel in order to heat the sample inside the channel; and a liquid feeding system, which controls the pressure inside the channel of the reaction processing vessel in order to move the sample inside the channel. The liquid feeding system maintains the pressure inside the channel to be higher than the atmospheric pressure in the surrounding of the reaction processing vessel, more preferably 1 atm or higher, during a reaction process of the sample.
-
7.发明申请公开(公告)号：US20200157607A1
公开(公告)日：2020-05-21
申请号：US16782794
申请日：2020-02-05
Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY , KYORIN PHARMACEUTICAL CO., LTD.
Inventor： Hidenori NAGAI , Shunsuke FURUTANI , Yoshihisa HAGIHARA , Yusuke FUCHIWAKI
Abstract: The present invention provides a reciprocal-flow-type nucleic acid amplification device comprising: heaters capable of forming a denaturation temperature zone and an extension/annealing temperature zone; a fluorescence detector capable of detecting movement of a sample solution between the two temperature zones; a pair of liquid delivery mechanisms that allow the sample solution to move between the two temperature zones and that are configured to be open to atmospheric pressure when liquid delivery stops; a substrate on which the chip for nucleic acid amplification according to claim 2 can be placed; and a control mechanism that controls driving of each liquid delivery mechanism by receiving an electrical signal from the fluorescence detector relating to movement of the sample solution from the control mechanism; the device being capable of performing real-time PCR by measuring fluorescence intensity for each thermal cycle.
-
公开(公告)号：US20180274019A1
公开(公告)日：2018-09-27
申请号：US15993814
申请日：2018-05-31
Applicant: Nippon Sheet Glass Company, Limited , National Institute of Advanced Industrial Science and Technology , Go!Foton, Inc.
Inventor： Takashi FUKUZAWA , Hidenori NAGAI , Naofumi NISHIZAWA
IPC: C12Q1/6848 , C12Q1/686 , B01L7/00 , G01N21/64 , B01L3/00
CPC classification number: C12Q1/6848 , B01L3/502 , B01L7/52 , B01L2200/0689 , B01L2300/0681 , B01L2300/0883 , B01L2300/14 , B01L2400/0487 , C12M1/00 , C12Q1/686 , G01N21/64 , G01N35/08 , G01N37/00
Abstract: A PCR reaction vessel includes: a substrate; a channel formed on the substrate; a pair of filters, a first filter and a second filter, provided at respective ends of the channel; a pair of air communication ports, a first air communication port and a second air communication port, that communicate with the channel through the first filter and the second filter; a thermal cycle region formed between the first filter and the second filter in the channel; a branch point formed between the first filter and the second filter in the channel; a branched channel whose one end is connected to the branch point; and a sample introduction port formed at the other end of the branched channel.
-
公开(公告)号：US20170370924A1
公开(公告)日：2017-12-28
申请号：US15688280
申请日：2017-08-28
Inventor： Makoto FUJIMAKI , Nobuko FUKUDA , Kenichi NOMURA , Hidenori NAGAI , Toshihiko OOIE
IPC: G01N33/543 , G01N21/552 , G01N21/64
CPC classification number: G01N33/54373 , G01N21/553 , G01N21/6428 , G01N21/648 , G01N2021/6439
Abstract: [Problem] To provide a target substance detection chip, a target substance detection device, and a target substance detection method, that can be manufactured easily in a small size at low costs with reduction of the number of parts involved in the detection chip constituted by an optical prism and a detection plate used for a SPR sensor and an optical waveguide mode sensor, that can detect a target substance quickly with high sensitivity, and in which an analyte liquid is easily delivered.[Solution] A target substance detection chip of the present invention includes: a plate-like transparent base portion which allows light to pass therethrough; and a flow path which is formed in one surface of the transparent base portion as a groove and through which an analyte liquid verifying a presence of a target substance is delivered in a length direction of the groove, wherein the flow path is formed such that at least an electric field enhancement layer is disposed on an inner surface of a groove portion formed to at least partly have inclined surfaces appearing in cross section to be inclined at a gradient to the surface of the transparent base portion, and wherein a part or entirety of an uppermost surface of the groove which contacts the analyte liquid serves as a detection surface for the target substance.
-
-
-
-
-
-
-
-
Search Results
9
records
(took 0.02 seconds)
