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公开(公告)号：US20140206045A1
公开(公告)日：2014-07-24
申请号：US14231062
申请日：2014-03-31
Applicant: Novozymes A/S
Inventor： Lan Tang , Wenping Wu , Junxin Duan , Pia Francke Johannesen
CPC classification number: C12N9/242 , A21D8/042 , C12C11/003 , C12N9/2414 , C12P7/02 , C12P19/12 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
Abstract: The present invention relates to an isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO: 4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO: 3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.
Abstract translation: 本发明涉及一种分离的多核苷酸，其包含编码具有α-淀粉酶活性的多肽的开放阅读框，所述多肽选自：a）包含与氨基酸具有至少70％同一性的氨基酸序列的多肽 SEQ ID NO：4的22至450; b）一种多肽，其包含氨基酸序列，该氨基酸序列与插入存在于根据布达佩斯条约与DSMZ保藏的大肠杆菌宿主的质粒存在的质粒的多核苷酸编码的多肽编码的多肽相同， 15334; c）由多核苷酸编码的多肽，其包含与SEQ ID NO：3中的位置68至1417所示的序列具有至少70％同一性的核苷酸序列; 和d）具有α-淀粉酶活性的（a），（b）或（c）的片段。
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公开(公告)号：US20160208230A1
公开(公告)日：2016-07-21
申请号：US15092044
申请日：2016-04-06
Applicant: NOVOZYMES A/S
Inventor： Lan Tang , Wenping Wu , Junxin Duan , Pia Francke Johannesen
CPC classification number: C12N9/242 , A21D8/042 , C12C11/003 , C12N9/2414 , C12P7/02 , C12P19/12 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
Abstract: The present invention relates to an isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO: 4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO: 3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.
Abstract translation: 本发明涉及一种分离的多核苷酸，其包含编码具有α-淀粉酶活性的多肽的开放阅读框，所述多肽选自：a）包含与氨基酸具有至少70％同一性的氨基酸序列的多肽 SEQ ID NO：4的22至450; b）一种多肽，其包含氨基酸序列，该氨基酸序列与插入存在于根据布达佩斯条约与DSMZ保藏的大肠杆菌宿主的质粒存在的质粒的多核苷酸编码的多肽编码的多肽相同， 15334; c）由多核苷酸编码的多肽，其包含与SEQ ID NO：3中的位置68至1417所示的序列具有至少70％同一性的核苷酸序列; 和d）具有α-淀粉酶活性的（a），（b）或（c）的片段。
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公开(公告)号：US09394533B2
公开(公告)日：2016-07-19
申请号：US14231062
申请日：2014-03-31
Applicant: NOVOZYMES A/S
Inventor： Lan Tang , Wenping Wu , Junxin Duan , Pia Francke Johannesen
IPC: C12N9/30 , C12N9/34 , C12N15/00 , C12P21/06 , C12P19/34 , C12N1/20 , C12P7/06 , C12P19/14 , C07H21/04 , C07K1/00 , C12N9/26
CPC classification number: C12N9/242 , A21D8/042 , C12C11/003 , C12N9/2414 , C12P7/02 , C12P19/12 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
Abstract: The present invention relates to an isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO: 4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO: 3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.
Abstract translation: 本发明涉及一种分离的多核苷酸，其包含编码具有α-淀粉酶活性的多肽的开放阅读框，所述多肽选自：a）包含与氨基酸具有至少70％同一性的氨基酸序列的多肽 SEQ ID NO：4的22至450; b）一种多肽，其包含氨基酸序列，该氨基酸序列与插入存在于根据布达佩斯条约与DSMZ保藏的大肠杆菌宿主的质粒存在的质粒的多核苷酸编码的多肽编码的多肽相同， 15334; c）由多核苷酸编码的多肽，其包含与SEQ ID NO：3中的位置68至1417所示的序列具有至少70％同一性的核苷酸序列; 和d）具有α-淀粉酶活性的（a），（b）或（c）的片段。
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公开(公告)号：US09938512B2
公开(公告)日：2018-04-10
申请号：US15092044
申请日：2016-04-06
Applicant: NOVOZYMES A/S
Inventor： Lan Tang , Wenping Wu , Junxin Duan , Pia Francke Johannesen
IPC: C12N9/30 , C12N9/24 , C12N15/00 , C12P21/06 , C12P19/34 , C12N1/20 , C12N1/00 , C12P7/06 , C12N9/22 , C07H21/04 , C07K1/00 , C12N9/26 , C12P19/14 , A21D8/04 , C12C11/00 , C12P7/02 , C12P19/12 , D06M16/00
CPC classification number: C12N9/242 , A21D8/042 , C12C11/003 , C12N9/2414 , C12P7/02 , C12P19/12 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
Abstract: The present invention relates to an isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO: 4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO: 3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.
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