公开(公告)号：US20240084263A1

公开(公告)日：2024-03-14

申请号：US18274532

申请日：2022-03-07

Applicant: POSTECH RESEARCH AND BUSINESS DEVELOPMENT FOUNDATION

Inventor： Ji Soo KIM ,  Jin Ah JANG ,  Dong Woo CHO

IPC: C12N5/071 ,  B33Y70/00 ,  C12N5/00

CPC classification number: C12N5/0679 ,  B33Y70/00 ,  C12N5/0068 ,  C12N2509/00

Abstract: The present invention relates to a method for decellularization of porcine stomach mucosal tissue for culturing stomach tissue or stomach cancer tissue, a composition for producing a scaffold, and a method for culturing tissue, and particularly, to: a method for decellularization of porcine stomach mucosal tissue comprising chopping porcine stomach mucosal tissue and performing first treatment, second treatment, washing, and third treatment; a decellularization product composed of porcine stomach mucosal tissue decellularized by the method; a composition for producing a stomach tissue or stomach cancer tissue culture scaffold containing a pepsin digest of the decellularization product; and a method for culturing stomach tissue or stomach cancer tissue comprising adding stomach cells or stomach cancer cells to the composition, followed by shaping to produce a tissue culture scaffold containing stomach cells or stomach cancer cells, and incubating the scaffold in a medium.